Screening and characterization of PHA producing bacteria from sewage water identifying Bacillus paranthracis RSKS-3 for bioplastic production
BMC Microbiology,
Journal Year:
2025,
Volume and Issue:
25(1)
Published: March 14, 2025
Abstract
Polyhydroxyalkanoate
(PHA)
as
bioplastic
is
considered
a
replacement
for
conventional
plastic
due
to
its
more
beneficial
properties.
The
ability
of
PHA
biodegrade
in
shorter
period
major
advantage.
Different
sewage
water
samples
were
collected
from
the
Budha
Nala
near
Maheru
regions
Punjab.
PHA-producing
bacteria
isolated
using
minimal
salt
media
supplemented
with
Nile
blue.
Further
screening
was
carried
out
Sudan
Black
B
stain
and
red
stain.
positive
isolates
characterized
gram
reaction,
motility,
biochemical
tests.
individual
later
screened
maximum
accumulation
glucose.
extracted
FTIR,
XRD,
SEM,
UV
spectroscopy,
NMR,
TGA.
Twenty-six
different
on
Upon
stain,
nineteen
showed
black
granules
orange
fluorescence
bodies
under
100X
magnification
that
confirmed
polyhydroxyalkanoates.
tests
partially
belonging
Bacillus
genus.
All
produced
granular
form,
however,
isolate
P-3
production
0.068
g/L.
FTIR
XRD
chemical
crystallinity
studies
spectroscopy
by
analyzing
absorption
spectra
at
235
nm
standard
crotonic
acid
sulfuric
conversion
acid.
P-3,
paranthracis
RSKS-3
first
reported
bacterium
produce
necessary
optimize
efficiency
yield.
Language: Английский
Synthetic biology for medical biomaterials
Deleted Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 29, 2025
Abstract
After
more
than
20
years
of
development,
synthetic
biology
has
emerged
as
an
interdisciplinary
field
that
integrates
biology,
medicine,
mathematics,
and
engineering.
By
constructing
regulating
genetic
elements,
networks,
pathways,
artificially
engineered
bacteria,
cells,
or
viruses
can
directly
interact
with
the
human
body
to
enable
disease
treatment
via
biology.
Additionally,
platforms
have
been
employed
in
production
medical
biomaterials
(MBMs),
indirectly
contributing
maintenance
health.
In
this
review,
we
present
a
range
typical
MBMs
derived
from
platforms,
including
polylactic
acid,
polyhydroxyalkanoates,
hyaluronic
collagen,
poly(β‐hydroxybutyrate),
poly(β‐malic
acid),
poly‐γ‐glutamic
alginate,
chitosan,
bacterial
cellulose,
antimicrobial
peptides.
We
also
introduce
key
techniques
tools
involved,
such
chassis
cell
design,
gene
expression
regulation
editing
represented
by
CRISPRi,
metabolic
engineering,
morphology
cell‐free
systems.
Furthermore,
summarize
recent
advancements
strategies
enhancing
cost‐reduction,
biosynthesis
novel
materials,
material
characteristics
diversity,
minimizing
toxicity
biosynthetic
systems,
designing
living
materials
research
applications
clinical
translation
for
MBMs.
Finally,
discuss
emerging
trends
may
shape
future
biomedical
Language: Английский