Enzyme and Microbial Technology, Journal Year: 2024, Volume and Issue: 180, P. 110485 - 110485

Published: July 16, 2024

Gram-positive Bacillus subtilis is a model organism for the biotechnology industry and has recently been characterized as weakly electroactive in both planktonic cultures biofilms. Increasing extracellular electron transfer (EET) rate B. biofilms will help to develop an efficient microbial electrochemical technology (MET) improve bioproduction of high-value metabolites under electrofermentative conditions. In our previous work, we have shown that addition compatible solute precursors such choline chloride (ChCl) growth medium formulation increases current output biofilm formation subtilis. this utilized low-carbon tryptone yeast extract with added salts further expose salt stress observe osmoregulatory and/or nutritional effects D-sorbitol/choline (1:1 mol mol−1) deep eutectic solvents (DESs) on electroactivity formed biofilm. The results show ChCl D-sorbitol alleviate osmotic induced by NaH2PO4 KH2PO4 boost production. This probably due osmoprotective effect ChCl, precursor osmoprotectant glycine betaine, induction exopolymeric substances within Since high ionic strength media are commonly used biotechnology, combination ChCl-containing DESs could enhance biofilm-based electrofermentation processes bring significant benefits biotechnological applications.

Language: Английский