Persistent organic pollutants dysregulate energy homeostasis in human ovaries in vitro

Environment International, Journal Year: 2024, Volume and Issue: 187, P. 108710 - 108710

Published: April 27, 2024

Exposure to persistent organic pollutants (POPs), such as dichlorodiphenyltrichloroethane (DDT) and polychlorinated biphenyls (PCBs), has historically been linked population collapses in wildlife. Despite international regulations, these legacy chemicals are still currently detected women of reproductive age, their levels correlate with reduced ovarian reserve, longer time-to-pregnancy, higher risk infertility. However, the specific modes action underlying associations remain unclear. Here, we examined effects five commonly occurring POPs - hexachlorobenzene (HCB), p,p'-dichlorodiphenyldichloroethylene (DDE), 2,3,3',4,4',5-hexachlorobiphenyl (PCB156), 2,2',3,4,4',5,5'-heptachlorobiphenyl (PCB180), perfluorooctane sulfonate (PFOS) mixture on human ovaries vitro. We exposed cancer cell lines COV434, KGN, PA1 well primary cells for 24 h, tissue containing unilaminar follicles 6 days. RNA-sequencing samples concentrations covering epidemiologically relevant revealed significant gene expression changes related central energy metabolism cells, indicating glycolysis, oxidative phosphorylation, fatty acid metabolism, reactive oxygen species potential shared targets POP exposures cells. Alpha-enolase (ENO1), lactate dehydrogenase A (LDHA), cytochrome C oxidase subunit 4I1 (COX4I1), ATP synthase F1 alpha (ATP5A), glutathione peroxidase 4 (GPX4) were validated through qPCR additional culture experiments KGN. In cultures, observed exposure follicle growth atresia protein expression. All exposures, except PCB180, decreased proportion increased atresia. Immunostaining confirmed altered LDHA, ATP5A, GPX4 tissues. Moreover, modified production KGN culture. conclusion, our results demonstrate disruption cellular a novel mode POP-mediated interference ovaries.

Language: Английский

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Elucidating the degradation mechanisms of perfluorooctanoic acid and perfluorooctane sulfonate in various environmental matrices: a review of green degradation pathways

Environmental Geochemistry and Health, Journal Year: 2024, Volume and Issue: 46(9)

Published: July 29, 2024

Language: Английский

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Unveiling the Research Void: Exploring the Reproductive Effects of PFAS Compounds on Male Health

Advances in experimental medicine and biology, Journal Year: 2025, Volume and Issue: unknown, P. 127 - 162

Published: Jan. 1, 2025

Language: Английский

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The characterization and performance of a core–shell structured nanoplatform for fluorescence turn-on sensing and selective removal of perfluorooctane substance

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: 340, P. 126356 - 126356

Published: May 9, 2025

Language: Английский

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Integrative network and computational toxicology reveal the molecular mechanisms in PFOA-induced spermatogenic disorder

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 386, P. 125754 - 125754

Published: May 13, 2025

Language: Английский

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Nicotinamide mononucleotide enhances the developmental potential of mouse early embryos exposed to perfluorooctanoic acid

Reproductive Toxicology, Journal Year: 2024, Volume and Issue: 132, P. 108762 - 108762

Published: Nov. 28, 2024

Language: Английский

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How Per- and Poly-Fluoroalkyl Substances Affect Gamete Viability and Fertilization Capability: Insights from the Literature

Journal of Xenobiotics, Journal Year: 2024, Volume and Issue: 14(2), P. 651 - 678

Published: May 17, 2024

There has been emerging research linking per- and poly-fluoroalkyl substances (PFAS) to gamete viability fertility. PFAS, prevalent in the environment water supplies, undergo slow degradation due their C-F bond a long half-life (2.3–8.5 years). In females, PFAS inhibit hypothalamic–pituitary–gonadal (HPG) axis, reducing follicle-stimulating hormone (FSH) luteinizing (LH) levels, leading inhibition of androgen estradiol production. have found cause detrimental effects on egg quality through impairing folliculogenesis. males, can impair sperm motility morphology: two fundamental qualities successful fertilization. exposure proven testosterone production, capacitation, acrosomal reaction. After fertilization, results embryos also investigated, showing reduced development blastocyst stage. The aim this review is report main findings literature impact competency fertilization capability by highlighting key studies both male female We that there significant evidence demonstrating negative impacts fertility after exposure. At high doses, these environmentally abundant widespread compounds significantly affect human

Language: Английский

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Adolescent F-53B exposure induces ovarian toxicity in rats: Autophagy-apoptosis interplay

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 951, P. 175609 - 175609

Published: Aug. 18, 2024

Language: Английский

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Possible mechanisms for adverse effects on zebrafish sperm and testes associated with low-level chronic PFOA exposure

Aquatic Toxicology, Journal Year: 2024, Volume and Issue: 276, P. 107108 - 107108

Published: Sept. 26, 2024

Language: Английский

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Cordycepin improves hyperactivation and acrosome reaction through adenosine receptors during human sperm capacitation in vitro

Reproductive Biology and Endocrinology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 12, 2024

Sperm capacitation is a prerequisite for natural or in vitro fertilization. After capacitation, sperm become hyperactivated and undergo an acrosome reaction, which helps them penetrate the oocyte. Cordycepin, bioactive compound first isolated from Cordyceps militaris, adenosine analog with numerous physiological activities. However, its effects on remain unclear. This study aims to elucidate mechanisms of cordycepin human capacitation.

Language: Английский

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