Identifying in vitro toxicity testing approaches for (novel) proteins in the context of food and feed risk assessment DOI Open Access

Louis‐Marie Cobigo,

Denis Habauzit, Kahina Mehennaoui

et al.

EFSA Supporting Publications, Journal Year: 2024, Volume and Issue: 21(11)

Published: Nov. 1, 2024

Abstract This report documents the outcomes of EFSA procurement (OC/EFSA/NIF/2022/01) aimed at identifying in vitro toxicity testing approaches for (novel) proteins context food and feed safety assessment. In present report, we an integrated strategy evaluation novel/toxic proteins. A text‐mining approach was used to create a literature database toxic associated with retrieved from UniProt KB using search term “Toxin activity”. It shown that are produced by relatively limited phylogenetic subset, including, among others, bacteria, insects, serpents, molluscs, fungi. Toxicological effects these generally conserved within groups. Analysis performed GO analysis as well based approach. Relevant tests address quantify were identified evaluated their applicability strategy. stepwise developed. As first step, initial silico prediction is carried out (Step 1). followed battery assays primary mechanisms 2). If concern arises Step 2 tests, use relevant model systems explore potential target organ required 3). Knowledge gaps have been recommendations provided strategies, particular Some involve selection integration standardized, digestion reflective passage through digestive tract, strategy, thorough assessment suitability new methodologies regulatory To accelerate incorporation NAMs protein safety, case studies proof concept projects needed demonstrate utility effectiveness strategies

Language: Английский

Identifying in vitro toxicity testing approaches for (novel) proteins in the context of food and feed risk assessment DOI Open Access

Louis‐Marie Cobigo,

Denis Habauzit, Kahina Mehennaoui

et al.

EFSA Supporting Publications, Journal Year: 2024, Volume and Issue: 21(11)

Published: Nov. 1, 2024

Abstract This report documents the outcomes of EFSA procurement (OC/EFSA/NIF/2022/01) aimed at identifying in vitro toxicity testing approaches for (novel) proteins context food and feed safety assessment. In present report, we an integrated strategy evaluation novel/toxic proteins. A text‐mining approach was used to create a literature database toxic associated with retrieved from UniProt KB using search term “Toxin activity”. It shown that are produced by relatively limited phylogenetic subset, including, among others, bacteria, insects, serpents, molluscs, fungi. Toxicological effects these generally conserved within groups. Analysis performed GO analysis as well based approach. Relevant tests address quantify were identified evaluated their applicability strategy. stepwise developed. As first step, initial silico prediction is carried out (Step 1). followed battery assays primary mechanisms 2). If concern arises Step 2 tests, use relevant model systems explore potential target organ required 3). Knowledge gaps have been recommendations provided strategies, particular Some involve selection integration standardized, digestion reflective passage through digestive tract, strategy, thorough assessment suitability new methodologies regulatory To accelerate incorporation NAMs protein safety, case studies proof concept projects needed demonstrate utility effectiveness strategies

Language: Английский

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