Comparison of Trichoderma longibrachiatum Xyloglucanase Production Using Tamarind (Tamarindus indica) and Jatoba (Hymenaea courbaril) Seeds: Factorial Design and Immobilization on Ionic Supports DOI Creative Commons
Alex Graça Contato, Ana Cláudia Vici, Vanessa Elisa Pinheiro

et al.

Fermentation, Journal Year: 2022, Volume and Issue: 8(10), P. 510 - 510

Published: Oct. 2, 2022

Xyloglucan (XG) is the predominant hemicellulose in primary cell wall of superior plants. It has a fundamental role controlling stretching and expansion plant wall. There are five types enzymes known to cleave linear chain xyloglucan, most well-known xyloglucanase (XEG). The immobilization process can be used solve problems related stability, besides economic benefits brought by possibility its repeated use recovery. Therefore, this study aims at optimization production Trichoderma longibrachiatum using central composite rotatable design (CCRD) with tamarind jatoba seeds as carbon sources, well XEG on ionic supports, such MANAE (monoamine-N-aminoethyl), DEAE (diethylaminoethyl)-cellulose, CM (carboxymethyl)-cellulose, PEI (polyethyleneimine). High concentrations sources (1.705%), temperature 30 °C under agitation for 72 h, were favorable conditions activity from T. respect both sources. However, showed 23.5% higher compared seeds. source was chosen continue experiments. scaling up Erlenmeyer flasks bioreactor increased 1.27-fold (1.040 ± 0.088 U/mL). Regarding biochemical characterization crude extract, optimal range 50–55 °C, pH 5.0. stabilities temperature, not stable prolonged periods, which crucial immobilizing it resins. best efficiency CM-cellulose DEAE-cellulose, activities 1.16 0.89 U/g derivative (enzyme plus support), respectively. This describes, first time literature, fungal these supports.

Language: Английский

Current perspective in research and industrial applications of microbial cellulases DOI
Priya Sutaoney, Sachchida Nand, Sakshi Sinha

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130639 - 130639

Published: March 5, 2024

Language: Английский

Citations

30

Recent advances on lignocellulosic bioresources and their valorization in biofuels production: Challenges and viability assessment DOI Creative Commons
Neha Srivastava, Rajeev Singh, Pardeep Singh

et al.

Environmental Technology & Innovation, Journal Year: 2023, Volume and Issue: 29, P. 103037 - 103037

Published: Jan. 27, 2023

Lignocellulosic biomass (LCB) waste materials are abundant in nature, and because of their high cellulose content, they rank among the most widely accessible preferred feedstocks for development cost-effective biorefineries. The main obstacle to long-term viability this valorization at pilot size, however, is complexity structural composition these wastes lack a suitable bioprocess economical efficient biotransformation. current review investigates potential economically viable environmentally friendly biotransformation LCB into cellulolytic enzymes biofuels generation technologies. focuses on synthesis energy from through Based update progress, information constraint that currently exists structure successful limitation surmounted have also been evaluated. To improve overall sustainable scale, other possible recommendations proposed. Such valorizations can contribute circular economy future applications.

Language: Английский

Citations

32

Enhanced production of mycelium biomass and exopolysaccharides of Pleurotus ostreatus by integrating response surface methodology and artificial neural network DOI

Arman Hamza,

Abdul Khalad,

Devarai Santhosh Kumar

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 399, P. 130577 - 130577

Published: March 11, 2024

Language: Английский

Citations

11

Enzymes Produced by the Genus Aspergillus Integrated into the Biofuels Industry Using Sustainable Raw Materials DOI Creative Commons

Fernando Enrique Rosas-Vega,

Roberta Pozzan,

Walter José Martínez-Burgos

et al.

Fermentation, Journal Year: 2025, Volume and Issue: 11(2), P. 62 - 62

Published: Feb. 1, 2025

Renewable energy sources, such as biofuels, represent promising alternatives to reduce dependence on fossil fuels and mitigate climate change. Their production through enzymatic hydrolysis has gained relevance by converting agro-industrial waste into fermentable sugars residual oils, which are essential for the generation of bioethanol biodiesel. The fungus Aspergillus stands out a key source enzymes, including cellulases, xylanases, amylases, lipases, crucial breakdown biomass oils produce fatty acid methyl esters (FAME). This review examines current state these technologies, highlighting significance in conversion energy-rich materials. While process holds significant potential, it faces challenges high costs associated with final processing stages. Agro-industrial is proposed an resource support circular economy, thereby eliminating reliance non-renewable resources processes. Furthermore, advanced pretreatment technologies—including biological, physical, physicochemical methods, well use ionic liquids—are explored enhance efficiency. Innovative genetic engineering strains enzyme encapsulation, promise optimize sustainable biofuel addressing advancing this technology towards large-scale implementation.

Language: Английский

Citations

1

Machine Learning: A Suitable Method for Biocatalysis DOI Open Access
Pedro Sampaio, Pedro Fernandes

Catalysts, Journal Year: 2023, Volume and Issue: 13(6), P. 961 - 961

Published: June 1, 2023

Biocatalysis is currently a workhorse used to produce wide array of compounds, from bulk fine chemicals, in green and sustainable manner. The success biocatalysis largely thanks an enlargement the feasible chemical reaction toolbox. This materialized due major advances enzyme screening tools methods, together with high-throughput laboratory techniques for biocatalyst optimization through engineering. Therefore, enzyme-related knowledge has significantly increased. To handle large number data now available, computational approaches have been gaining relevance biocatalysis, among them machine learning methods (MLMs). MLMs use algorithms learn improve experience automatically. review intends briefly highlight contribution within biochemical engineering bioprocesses present key aspects scope related fields, mostly readers non-skilled mind. Accordingly, brief overview basic concepts underlying are presented. complemented steps build model followed by insights into types intelligently analyse data, identify patterns develop realistic applications bioprocesses. Notwithstanding, given this review, some recent illustrative examples protein engineering, production, formulation provided, future developments suggested. Overall, it envisaged that will provide how these assets more efficient biocatalysis.

Language: Английский

Citations

17

Designing microbial cellulases using genetic engineering approach: A promising strategy towards zero-waste cellulosic biorefinery DOI
Komal Agrawal,

Lakshana G. Nair,

Venkatesh Chaturvedi

et al.

Biocatalysis and Agricultural Biotechnology, Journal Year: 2023, Volume and Issue: 52, P. 102830 - 102830

Published: Aug. 28, 2023

Language: Английский

Citations

14

Statistically assisted optimisation for the simultaneous production of Trichoderma harzianum and Aspergillus fumigatus cellulolytic enzymes DOI
Hwee Li Teo, Roswanira Abdul Wahab,

Mohd Hamdi Zainal-Abidin

et al.

Biomass Conversion and Biorefinery, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 2, 2024

Language: Английский

Citations

6

Microbial enzyme production: Unlocking the potential of agricultural and food waste through solid-state fermentation DOI
Ashutosh Khaswal, Santosh Kumar Mishra,

Neha Chaturvedi

et al.

Bioresource Technology Reports, Journal Year: 2024, Volume and Issue: 27, P. 101880 - 101880

Published: June 15, 2024

Language: Английский

Citations

5

Synergistic effect of cellulo-xylanolytic and laccase enzyme consortia for improved deinking of waste papers DOI

Guddu Kumar Gupta,

Rajeev Kumar Kapoor, Deepak Chhabra

et al.

Bioresource Technology, Journal Year: 2024, Volume and Issue: 408, P. 131173 - 131173

Published: July 29, 2024

Language: Английский

Citations

5

Statistical Evaluation for the Formulation of Low-Cost Single Cell Protein Production in Food Waste Medium by Pichia occidentalis: A Sustainable Valorization Approach DOI
P. Sagar,

Gopalkumar G. Raol,

Darshankumar Prajapati

et al.

Waste and Biomass Valorization, Journal Year: 2024, Volume and Issue: 15(6), P. 3457 - 3475

Published: Jan. 16, 2024

Language: Английский

Citations

4