Fucose is an essential feature in cryoprotective polysaccharides DOI Creative Commons
Bruno M. Guerreiro, Patrícia Concórdio‐Reis,

Helena Pericão

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 14, 2023

Abstract Biological cryopreservation often involves using a cryoprotective agent (CPA) to mitigate lethal physical stressors cells endure during freezing and thawing, but effective CPA concentrations are cytotoxic. Hence, natural polysaccharides have been studied as biocompatible alternatives. Our current investigation 26 potential CPA, probing correlations between post-thaw metabolic viability (PTV) of cryopreserved Vero monomeric composition. The best performing contained significant fucose amounts, resulting in average PTV 2.8-fold (up 3.1-fold) compared 0.8-fold 2.2-fold for all non-cryoprotective polysaccharides, respectively, outperforming the optimized commercial CryoStor™ CS5 formulation (2.6-fold). Stoichiometrically, balance (18–35.7 mol%), uronic acids (UA) (13.5–26 mol%) high molecular weight (MW > 1 MDa) generated optimal PTV. To deconvolute multiple variable effects, principal component analysis (PCA) coupled K -means clustering was performed. Two major mechanisms action explained variability: charge-dependent effect contrasting charged acid neutral monomer compositions, MW-scaled charge-independent mechanism exclusively attributed fucose. Ultimately, our research showed critical role plays enhancing cellular outcomes, disputing previous assumptions polyanionicity being sole governing predictor cryoprotection, highlighting fucose-rich polyanionic polysaccharides.

Language: Английский

Exopolysaccharide production by salt-tolerant bacteria: Recent advances, current challenges, and future prospects DOI
Longzhan Gan, Xin Huang, Zhicheng He

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 264, P. 130731 - 130731

Published: March 11, 2024

Language: Английский

Citations

10
Challenges and opportunities in bioprocessing of gellan gum: A review DOI
Nageswar Sahu, Biswanath Mahanty, Dibyajyoti Haldar

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 276, P. 133912 - 133912

Published: July 16, 2024

Language: Английский

Citations

7
Production and characterization of novel marine black yeast’s exopolysaccharide with potential antiradical and anticancer prospects DOI Creative Commons
Eman H. Zaghloul, Hala H. Abd El Latif, Asmaa Elsayis

et al.

Microbial Cell Factories, Journal Year: 2024, Volume and Issue: 23(1)

Published: Feb. 22, 2024

Abstract The marine black yeasts are characterized by the production of many novel protective substances. These compounds increase their physiological adaptation to multi-extreme environmental stress. Hence, exopolysaccharide (EPS) producing yeast SAHE was isolated in this study. It molecularly identified as Hortaea werneckii (identity 98.5%) through ITS1 and ITS4 gene sequencing analysis. physicochemical properties SAHE-EPS were investigated FTIR, GC-MS, TGA, ESM, EDX analysis, revealing its heteropolysaccharide nature. found be thermostable mainly consists sucrose, maltose, cellobiose, lactose, galactose. Furthermore, it exhibited an amorphous texture irregular porous surface structure. showed significant antiradical activity, demonstrated DPPH radical scavenging assay, IC50 recorded 984.9 μg/mL. In addition, outstanding anticancer activity toward A549 human lung cancer cell line (IC50 = 22.9 μg/mL). Conversely, demonstrates minimal cytotoxicity WI-38 normal 203 μg/mL), which implies safety. This study represents initial attempt isolate characterize chemical EPS produced H. a promising agent.

Language: Английский

Citations

4
The structure, characterization and immunomodulatory potential of exopolysaccharide produced by Planococcus rifietoensis AP-5 from deep-sea sediments of the Northwest Pacific DOI
Xin Hu, Shanshan Zhao,

Fengshu Li

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 245, P. 125452 - 125452

Published: June 16, 2023

Language: Английский

Citations

11
A genome-scale metabolic model for the denitrifying bacterium Thauera sp. MZ1T accurately predicts degradation of pollutants and production of polymers DOI Creative Commons
Diego Tec-Campos, Juan D. Tibocha‐Bonilla, Chunguo Jiang

et al.

PLoS Computational Biology, Journal Year: 2025, Volume and Issue: 21(1), P. e1012736 - e1012736

Published: Jan. 7, 2025

The denitrifying bacterium Thauera sp. MZ1T, a common member of microbial communities in wastewater treatment facilities, can produce different compounds from range carbon (C) and nitrogen (N) sources under aerobic anaerobic conditions. In these conditions, modifies its metabolism to that influence the community. particular, MZ1T produces exopolysaccharides with floc-forming properties, impacting physical disposition consortia efficiency nutrient assimilation by Under N-limiting decreases growth rate accelerates accumulation polyhydroxyalkanoate-related (PHA) including polyhydroxybutyrate (PHB), which plays fundamental role as C energy storage this β-proteobacterium. However, metabolic mechanisms employed assimilate catabolize many N conditions remain unknown. Systems biology approaches such genome-scale modeling have been successfully used unveil complex for various microorganisms. Here, we developed comprehensive model (M-model) (iThauera861), consisting 1,744 metabolites, 2,384 reactions, 861 genes. We validated experimentally using over 70 both iThauera861 achieved prediction accuracy 95% on close 85% aromatic M-model was subsequently deployed determine effects substrates, oxygen presence, C:N ratio production PHB (EPS), showing highest polymer yields are nucleotides amino acids This will help reveal processes ubiquitous species influences systems natural environments.

Language: Английский

Citations

0
Exploring simultaneous production of poly(3-hydroxybutyrate) and exopolysaccharides in cyanobacteria-rich microbiomes DOI Creative Commons
Beatriz Altamira-Algarra, Joan García Román, Cristiana A.V. Torres

et al.

New Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

The aim of this study was to explore the viability dual production poly(3-hydroxybutyrate) (PHB) and exopolysaccharides (EPS) by seven microbiomes rich in cyanobacteria. Our initial experiments involved screen for EPS-producing candidates examine impact salinity acetate on EPS synthesis. Salinity's known influence acetate's role enhancing PHB guided our parameter selection. Surprisingly, neither introduction an external carbon source (acetate) nor exposure abiotic stressor (salt) significantly altered synthesis rates, which ranged from 25 150mg·L-1, or its composition, with glucose being dominant sugar component. Scaling up a 3L photobioreactor, we achieved simultaneous biopolymer production, reaching 205mg·L-1 87mg·L-1 PHB. Additionally, presence uronic acid facilitated biomass flocculation, streamlining separation process, potentially reducing associated time costs.

Language: Английский

Citations

0
New insights on microbial extracellular polysaccharides: production, biological activity, and applications DOI

Suparna Sen,

Onkar Nath Tiwari,

Raj Kumar Arya

et al.

Biomass Conversion and Biorefinery, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Language: Английский

Citations

0
Bacterial Exopolysaccharides: A Comprehensive review on sustainable production from spoiled fruit wastes DOI
Mohamed Hemida Abd‐Alla, Elhagag Ahmed Hassan,

Esraa A. Mohammed

et al.

Biocatalysis and Agricultural Biotechnology, Journal Year: 2025, Volume and Issue: 66, P. 103607 - 103607

Published: May 6, 2025

Language: Английский

Citations

0
Elevated fucose content enhances the cryoprotective performance of anionic polysaccharides DOI
Bruno M. Guerreiro, Patrícia Concórdio‐Reis,

Helena Pericão

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 261, P. 129577 - 129577

Published: Jan. 21, 2024

Language: Английский

Citations

3
Bacterial cellulose production through the valorization of waste apple pulp and stale bread DOI Creative Commons
Asiyah Esmail, Maria Morais,

Ugur D. Yilmazer

et al.

Biomass Conversion and Biorefinery, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Abstract In this work, stale bread and waste apple pulp were used as feedstocks for the production of bacterial cellulose (BC). A glucose-rich solution was prepared from by dilute acid hydrolysis, while an extract comprising fructose glucose obtained pulp, which cultivating Komagataeibacter xylinus DSM 2004, either sole or supplemented with Hestrin-Schramm medium. Supplementation significantly improved BC production: 3.38 ± 0.09 g/L 2.07 0.22 hydrolysate. There no significant impact on chemical structure fiber diameter, but biopolymer produced had slightly higher crystallinity (CI = 59–69%) lower thermal degradation temperature ( T deg 341–350 ℃) than that hydrolysate 55%; 316–320 ℃). Moreover, supplementation led to preparation thicker membranes, Young’s modulus, tension, deformation at break water uptake capacity permeability O 2 CO . These results show are suitable cultivation conditions can be adjusted tailoring biopolymer’s mechanical barrier properties suit different applications.

Language: Английский

Citations

2