Multi‐Omics Analysis Reveals That AhNHL Contributes to Melatonin‐Mediated Cadmium Tolerance in Peanut Plants DOI Creative Commons
Rui Ren, Zenghui Cao, Xingli Ma

et al.

Journal of Pineal Research, Journal Year: 2025, Volume and Issue: 77(2)

Published: Feb. 12, 2025

Cadmium (Cd) pollution significantly hampers cleaner production of peanut (Arachis hypogaea L.). Therefore, exploring tolerance mechanisms to Cd stress and breeding low-Cd cultivars are urgently needed require intense efforts. Herein, multi-omics physiological studies reveal that multiple biological processes, including melatonin (MT) biosynthesis, involved in the plants. Exogenous MT was applied plants under stress, which decreased accumulation roots, shoots seeds for 40%-60%, promoted antioxidant capacity. Integrated investigation reveals MT-mediated is mainly attributed enhanced metabolism linolenic acid, glutathione (GSH), phenylpropanoid (lignin), development casparian strip root cell wall. Defense genes, such as non-race-specific disease resistance gene 1/harpininduced 1 (NDR1/HIN1)-like (AhNHL), were also upregulated by stress. Overexpression AhNHL tobacco reduced 37%-46%, alleviated photosynthesis-inhibition induced Transcriptomic analysis suggested confers through promoting biosynthesis GSH metabolism. Additionally, exogenous effectively improving sequestration capacity plants, while apply inhibitor (buthionine sulfoximine) exacerbated phytotoxicity. improves affecting expression genes transcription regulation, metal ion binding transport. Our findings provide novel insights into molecular underlying would facilitate cultivars.

Language: Английский

Multi‐Omics Analysis Reveals That AhNHL Contributes to Melatonin‐Mediated Cadmium Tolerance in Peanut Plants DOI Creative Commons
Rui Ren, Zenghui Cao, Xingli Ma

et al.

Journal of Pineal Research, Journal Year: 2025, Volume and Issue: 77(2)

Published: Feb. 12, 2025

Cadmium (Cd) pollution significantly hampers cleaner production of peanut (Arachis hypogaea L.). Therefore, exploring tolerance mechanisms to Cd stress and breeding low-Cd cultivars are urgently needed require intense efforts. Herein, multi-omics physiological studies reveal that multiple biological processes, including melatonin (MT) biosynthesis, involved in the plants. Exogenous MT was applied plants under stress, which decreased accumulation roots, shoots seeds for 40%-60%, promoted antioxidant capacity. Integrated investigation reveals MT-mediated is mainly attributed enhanced metabolism linolenic acid, glutathione (GSH), phenylpropanoid (lignin), development casparian strip root cell wall. Defense genes, such as non-race-specific disease resistance gene 1/harpininduced 1 (NDR1/HIN1)-like (AhNHL), were also upregulated by stress. Overexpression AhNHL tobacco reduced 37%-46%, alleviated photosynthesis-inhibition induced Transcriptomic analysis suggested confers through promoting biosynthesis GSH metabolism. Additionally, exogenous effectively improving sequestration capacity plants, while apply inhibitor (buthionine sulfoximine) exacerbated phytotoxicity. improves affecting expression genes transcription regulation, metal ion binding transport. Our findings provide novel insights into molecular underlying would facilitate cultivars.

Language: Английский

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