New Insight Into the Mechanism of Nitrite Enhancement on Heterotrophic Nitrification and Aerobic Denitrification Bacterium in Gene Expression DOI Open Access
Chunxia Zheng,

Xiongfeng Zhang,

Tengxia He

et al.

Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(3)

Published: March 1, 2025

ABSTRACT The growth and nitrogen metabolism of heterotrophic nitrification‐aerobic denitrification (HN‐AD) bacteria are affected by nitrite, but the mechanisms underlying this for strain Acinetobacter johnsonii EN‐J1 unclear. In study, addition 10 mg/L nitrite increased reduction rate ammonium 1.0 mg/L/h, 20 nitrate 3.9 mg/L/h. Compared with control, reductase activity, electron transfer adenosine triphosphate content were enhanced 142.0%, 278.0% 279.0%, respectively, in removal process after nitrite. whole genome was annotated genes such as narGHI , narK nsrR nirBD nasA glnA gltB gdhA amt . Transcriptome analysis showed that triggered significant upregulation several key pathways, including metabolism, tricarboxylic acid cycle, amino enhancing denitrification. expression ( narG hmp ) detected real‐time quantitative polymerase chain reaction. These results suggested enhances increasing genes, levels, which is important elucidating mechanism promotion biological efficiency.

Language: Английский

New Insight Into the Mechanism of Nitrite Enhancement on Heterotrophic Nitrification and Aerobic Denitrification Bacterium in Gene Expression DOI Open Access
Chunxia Zheng,

Xiongfeng Zhang,

Tengxia He

et al.

Environmental Microbiology, Journal Year: 2025, Volume and Issue: 27(3)

Published: March 1, 2025

ABSTRACT The growth and nitrogen metabolism of heterotrophic nitrification‐aerobic denitrification (HN‐AD) bacteria are affected by nitrite, but the mechanisms underlying this for strain Acinetobacter johnsonii EN‐J1 unclear. In study, addition 10 mg/L nitrite increased reduction rate ammonium 1.0 mg/L/h, 20 nitrate 3.9 mg/L/h. Compared with control, reductase activity, electron transfer adenosine triphosphate content were enhanced 142.0%, 278.0% 279.0%, respectively, in removal process after nitrite. whole genome was annotated genes such as narGHI , narK nsrR nirBD nasA glnA gltB gdhA amt . Transcriptome analysis showed that triggered significant upregulation several key pathways, including metabolism, tricarboxylic acid cycle, amino enhancing denitrification. expression ( narG hmp ) detected real‐time quantitative polymerase chain reaction. These results suggested enhances increasing genes, levels, which is important elucidating mechanism promotion biological efficiency.

Language: Английский

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