Evaluation of the Performance of an Ocular Surface Modulator Containing ST-Lysyal Versus Hyaluronic Acid Eyedrops in Patients with Dry Eye Disease: A Pilot Study DOI Creative Commons
Stefano Barabino, Andreia Rosa,

Sara Marini

et al.

Ophthalmology and Therapy, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Dry eye disease (DED) is a multifactorial condition of the ocular surface, primarily treated with tear substitutes, which do not fully restore natural functions. In this pilot study, we tested hypothesis that T-Lysyal (T-Lys) improves symptoms and signs in patients DED. Additionally, provide literature overview on effects T-Lys ophthalmology non-ophthalmology conditions to elucidate its mechanisms action. A double-masked, randomized study was conducted DED or hyaluronic acid (HA) 0.2% combined tamarind seeds polysaccharide (control group) for 2 months. Inclusion criteria were diagnosis lasting ≥ 6 months, Symptom Assessment Eye (SANDE) score 30, at least one following: fluorescein staining cornea (score 3, NEI scale), conjunctival (Lissamine Green, 3), breakup time (T-BUT) ≤ 10 s. Data from right used statistical analysis. PubMed search studies also performed without publication year restrictions. Twelve group 15 control completed (mean age 67 ± 11 years). treatment resulted significant improvements baseline (V0) months (V2) assessed by visual analogue scale (T-Lys: 4.58 2.57 mm V0, 2.92 1.38 V2, p < 0.05; control: 5.13 2.29 5.3 2.4 V2), T-BUT 2.58 1.31 s 3.58 1.37 3.07 1.43 3 1.13 corneal 2.16 4.17 1.41 3.70 1.4 2.19 V2). No adverse events reported. Literature findings supported T-Lys's efficacy managing both conditions. This provides first clinical evidence DED, supporting preclinical data highlighting potential as promising surface modulator. The registered ISRCTN registry Clinical Studies no. 13587929.

Language: Английский

ARL13B controls male reproductive tract physiology through primary and Motile Cilia DOI Creative Commons
Céline Augière, Gabriel Henrique Campolina-Silva, Aaran Vijayakumaran

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Oct. 14, 2024

ARL13B is a small regulatory GTPase that controls ciliary membrane composition in both motile cilia and non-motile primary cilia. In this study, we investigated the role of efferent ductules, tubules male reproductive tract essential to fertility which co-exist. We used genetically engineered mouse model delete Arl13b ductule epithelial cells, resulting compromised architecture functions. This deletion led disturbances reabsorptive/secretory processes triggered an inflammatory response. The observed phenotype showed significant variability linked partial infertility, highlighting importance maintaining proper physiological balance these ducts. These results emphasize dual functions regulating duct homeostasis, offering deeper insights into how related diseases affect system. disrupts structure leading impaired inflammation, subfertility. highlights ARL13B's critical physiology.

Language: Английский

Citations

2
The Role of Aquaporin 4 in Lacrimal Gland Ductal Fluid Secretion in Mice DOI Creative Commons

Gréta Elekes,

Virág Csapó,

Dóra Szarka

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(5), P. 30 - 30

Published: May 21, 2024

Purpose: Earlier reports highlighted the predominant presence of aquaporin 4 (AQP4) in duct cells rabbit lacrimal glands (LGs). Whereas significant alterations AQP4 mRNA levels have been observed experimental dry eye and during pregnancy, impact LG ductal fluid production remains unclear. In our recent work, role secretion was investigated utilizing wild type (WT) knock out (KO) mice. Methods: Tear assessed both WT KO animals. Immunostaining used to identify protein. Duct segments were harvested from LGs Fluid filtration permeability (Pf) quantified using video-microscopy. Ductal tear production, elicited by a cell-permeable cAMP analogue (8-bromo cAMP), carbachol, vasoactive intestinal peptide (VIP), phenylephrine (PHE), ducts. Results: A higher expression protein noted mice when compared acinar cells. Pf did not show notable between Carbachol comparable secretory responses ducts However, 8-bromo cAMP, VIP, PHE stimulation resulted decreased LGs. Conclusions: Our findings underscore functional relevance mouse seems play different roles secretions secretagogues. Specifically, cAMP-mediated, adrenergic agonist-related reduced

Language: Английский

Citations

1
The shared mechanism and potential diagnostic markers for premature ovarian failure and dry eye disease DOI Creative Commons
Xi Long, Zixuan Wu, Pengfei Jiang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 13, 2024

Abstract Premature ovarian failure (POF), which is often comorbid with dry eye disease (DED) a key issue affecting female health. Here, we explored the mechanism underlying POF and DED to further elucidate mechanisms improve treatment. Datasets related (GSE39501) (GSE44101) were identified from Gene Expression Omnibus (GEO) database subjected weighted gene coexpression network (WGCNA) differentially expressed genes (DEGs) analyses, respectively, intersection used obtain 158 in DED. Kyoto Encyclopedia of Genes Genomes (KEGG) Ontology (GO) analyses revealed that primarily DNA replication Cell cycle, respectively. Protein–Protein interaction (PPI) analysis obtained 15 hub genes: CDC20, BIRC5, PLK1, TOP2A, MCM5, MCM6, MCM7, MCM2, CENPA, FOXM1, GINS1, TIPIN, MAD2L1, CDCA3. To validate results, additional POF- DED-related datasets (GSE48873 GSE171043, respectively) selected. miRNAs-lncRNAs-genes machine learning methods genes. The DGIdb valdecoxib, amorfrutin A, kaempferitrin as potential drugs. Herein, bioinformatics perspective, providing new strategy explore comorbidity mechanism, opening up direction for diagnosis treatment

Language: Английский

Citations

1
Evaluation of the Performance of an Ocular Surface Modulator Containing ST-Lysyal Versus Hyaluronic Acid Eyedrops in Patients with Dry Eye Disease: A Pilot Study DOI Creative Commons
Stefano Barabino, Andreia Rosa,

Sara Marini

et al.

Ophthalmology and Therapy, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 19, 2024

Dry eye disease (DED) is a multifactorial condition of the ocular surface, primarily treated with tear substitutes, which do not fully restore natural functions. In this pilot study, we tested hypothesis that T-Lysyal (T-Lys) improves symptoms and signs in patients DED. Additionally, provide literature overview on effects T-Lys ophthalmology non-ophthalmology conditions to elucidate its mechanisms action. A double-masked, randomized study was conducted DED or hyaluronic acid (HA) 0.2% combined tamarind seeds polysaccharide (control group) for 2 months. Inclusion criteria were diagnosis lasting ≥ 6 months, Symptom Assessment Eye (SANDE) score 30, at least one following: fluorescein staining cornea (score 3, NEI scale), conjunctival (Lissamine Green, 3), breakup time (T-BUT) ≤ 10 s. Data from right used statistical analysis. PubMed search studies also performed without publication year restrictions. Twelve group 15 control completed (mean age 67 ± 11 years). treatment resulted significant improvements baseline (V0) months (V2) assessed by visual analogue scale (T-Lys: 4.58 2.57 mm V0, 2.92 1.38 V2, p < 0.05; control: 5.13 2.29 5.3 2.4 V2), T-BUT 2.58 1.31 s 3.58 1.37 3.07 1.43 3 1.13 corneal 2.16 4.17 1.41 3.70 1.4 2.19 V2). No adverse events reported. Literature findings supported T-Lys's efficacy managing both conditions. This provides first clinical evidence DED, supporting preclinical data highlighting potential as promising surface modulator. The registered ISRCTN registry Clinical Studies no. 13587929.

Language: Английский

Citations

0