Metabolic Characteristics of Sclera in Lens-Induced Myopic Guinea Pigs DOI Creative Commons
Xueqi Lin,

Changyue Zheng,

Jing Cong

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(13), P. 51 - 51

Published: Nov. 25, 2024

Purpose: Myopia development is closely associated with scleral tissue loss in both human and animal models. This research aimed to investigate the metabolic changes sclera of lens-induced myopic guinea pigs explore underlying mechanisms. Methods: was induced 2-week-old pigmented by having them wear −20-diopter lenses for 10 days, one eye designated as other control. Dual-platform untargeted metabolomics performed using gas chromatography–mass spectrometry (GC-MS) liquid (LC-MS) analyze sclera. Validation amino acid levels conducted via targeted metabolomics. Glycine intervention carried out fibroblasts myopia pig model evaluate its effects on COL1A1 synthesis progression. Results: After days myopia, GC-MS LC-MS analyses identified significant 29 85 metabolites sclera, respectively. Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis indicated that downregulation pyrimidine metabolism pathways crucial development. Targeted confirmed multiple acids were significantly reduced deficiency fibroblasts, glycine supplementation increased content. Animal studies demonstrated gavage inhibited axial elongation refractive error increase pigs, content, reversed reduction ferroptosis-related proteins GPX4 FTH1. Conclusions: Several acids, including glycine, l-isoleucine, l-serine, l-valine, along a marked pathways. can content inhibit progression reducing ferroptosis within suggesting could serve potential therapeutic myopia.

Language: Английский

Glutathione and a Pool of Metabolites Partly Related to Oxidative Stress Are Associated with Low and High Myopia in an Altered Bioenergetic Environment DOI Creative Commons

Salvador Mérida,

Amparo Návea, Carmen Desco

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(5), P. 539 - 539

Published: April 27, 2024

Oxidative stress forms part of the molecular basis contributing to development and manifestation myopia, a refractive error with associated pathology that is increasingly prevalent worldwide subsequently leads an upsurge in degenerative visual impairment due conditions are especially high myopia. The purpose our study was examine interrelation potential oxidative-stress-related metabolites found aqueous humor high-myopic, low-myopic, non-myopic patients within clinical study. We conducted cross-sectional study, selecting two sets undergoing cataract surgery. first set, which used analyze through NMR assay, comprised 116 patients. A total 59 were assigned quantified. PLS-DA score plot clearly showed separation minimal overlap between HM control samples. model allowed us determine 31 major metabolite differences groups. Complementary statistical analysis data six presented significant among experimental groups (p < 005). number these discovered have direct or indirect connection oxidative linked myopic eyes. Notably, we identified bioenergetic pathways undergone methylation, along choline its derivatives. second set consisted 73 who underwent glutathione assay. Here, variations both reduced oxidized all patient 0.01) for time. Axial length, status, complete ophthalmologic examination also recorded, interrelations metabolic parameters evaluated.

Language: Английский

Citations

5
Spatial heterogeneity of corneal biomechanical properties in myopia at nanoscale: A preliminary study DOI
Shu Juan Yang, Deng Hong, Jing Zhang

et al.

Experimental Eye Research, Journal Year: 2025, Volume and Issue: unknown, P. 110277 - 110277

Published: Feb. 1, 2025

Language: Английский

Citations

0
Molecular-level differentiation of myopic corneal tissue using Raman spectroscopy and multivariate analysis DOI

Juye Shi,

Yingbin Lin, Jing Wang

et al.

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: unknown, P. 126054 - 126054

Published: March 1, 2025

Language: Английский

Citations

0
The Preservation and Reuse of Lenticules Extracted via Small Incision Lenticule Extraction (SMILE): A Narrative Review DOI Creative Commons

Yaohua Zhang,

Jing Li, Zhiqing Wu

et al.

Bioengineering, Journal Year: 2025, Volume and Issue: 12(4), P. 380 - 380

Published: April 3, 2025

Small-incision lenticule extraction (SMILE) is a safe and effective procedure to correct myopia myopic astigmatism. The corneal stromal lenticules extracted from SMILE surgery have good light transmission, mechanical properties, biocompatibility, which are suitable for the treatment of variety diseases can solve problem donor cornea shortage. At present, no single method preserving has been universally accepted as ideal, preservation tissue integrity, optical transmittance, cellular viability, potential long-term storage remain key challenges. Current approaches include short-term methods such use dehydrating agents Optisol GS, strategies cryopreservation, hydrogel nutrient capsules, silicone oil. Standardized improve SMILE-derived substitute in clinical settings. reuse highly regarded research area, especially hyperopia, presbyopia, keratoconus, some ulcerative diseases, providing new possibilities addressing shortage improving surgical outcomes. Here, we review various applications SMILE-extracted lenticules, highlighting their shortages diseases.

Language: Английский

Citations

0
Nitric Oxide as a Novel Biomarker for Monitoring Heat Exposure Responses in High Glucose- maintained Keratinocytes DOI Open Access

Syeda Tayyiba Rahat,

Ilkka Miinalainen, Anni I. Nieminen

et al.

Published: June 3, 2024

Global warming and the associated increasing frequency of heatwaves are on rise leading to numerous health complications. Diabetic patients more vulnerable heat exposure than healthy individuals, owing their impaired sweating ability, however, little is known about how may impact function skin cells, commonly keratinocytes. Our recent report provided first proof-of-concept revealing that metabolic composition sweat EVs with changes in individuals type 2 diabetes patients. Given this shared cellular proprieties eccrine glands keratinocytes, we questioned whether mirror responses keratinocytes upon exposure. To this, exposed HaCat, which were maintained low (mimicking condition) high glucose acute (90 min), chronic (48 h), repetitive at 40 ⁰C. Through conducting a set experiments including targeted metabolomics approach, Greiss assay, western blotting analysis, electron microscopy studies. The data demonstrate results substantial metabolite levels belonging amino acids, pyrimidine metabolism, glycolysis pathways HaCat cells when compared those noted condition. Exploring molecular mechanisms through our discoveries highlight nitric oxide (NO) as novel biomarker level correlated duration frequency. Furthermore, reveal HSP-70 protein mitochondrial morphology consequently altered. This study provides evidence alterations collected from clinical proposes, for time, NO monitoring

Language: Английский

Citations

1
The potential role of amino acids in myopia: inspiration from metabolomics DOI
Ying Xie, Liyue Zhang,

Si‐Yi Chen

et al.

Metabolomics, Journal Year: 2024, Volume and Issue: 21(1)

Published: Dec. 15, 2024

Language: Английский

Citations

1
Biomarkers: Promising Tools Towards the Diagnosis, Prognosis, and Treatment of Myopia DOI Open Access
Varis Ruamviboonsuk, Carla Lança, Andrzej Grzybowski

et al.

Journal of Clinical Medicine, Journal Year: 2024, Volume and Issue: 13(22), P. 6754 - 6754

Published: Nov. 10, 2024

The prevalence of myopia, especially high continues to increase in several parts the world. Thus, use biomarkers for early myopia detection would be highly valuable clinical practice aiding prevention and control management. identification that can predict risk development, severity treatment response are paramount significance. In this review, we present current state research on highlighting challenges opportunities myopia. Although may have a role as indicators or response, adoption into only done when tests accuracy, easily measurable, minimally invasive, acceptable parents, children, eye care professionals. Large consortia studies necessary validate translate evidence practice.

Language: Английский

Citations

0
Metabolic Characteristics of Sclera in Lens-Induced Myopic Guinea Pigs DOI Creative Commons
Xueqi Lin,

Changyue Zheng,

Jing Cong

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(13), P. 51 - 51

Published: Nov. 25, 2024

Purpose: Myopia development is closely associated with scleral tissue loss in both human and animal models. This research aimed to investigate the metabolic changes sclera of lens-induced myopic guinea pigs explore underlying mechanisms. Methods: was induced 2-week-old pigmented by having them wear −20-diopter lenses for 10 days, one eye designated as other control. Dual-platform untargeted metabolomics performed using gas chromatography–mass spectrometry (GC-MS) liquid (LC-MS) analyze sclera. Validation amino acid levels conducted via targeted metabolomics. Glycine intervention carried out fibroblasts myopia pig model evaluate its effects on COL1A1 synthesis progression. Results: After days myopia, GC-MS LC-MS analyses identified significant 29 85 metabolites sclera, respectively. Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis indicated that downregulation pyrimidine metabolism pathways crucial development. Targeted confirmed multiple acids were significantly reduced deficiency fibroblasts, glycine supplementation increased content. Animal studies demonstrated gavage inhibited axial elongation refractive error increase pigs, content, reversed reduction ferroptosis-related proteins GPX4 FTH1. Conclusions: Several acids, including glycine, l-isoleucine, l-serine, l-valine, along a marked pathways. can content inhibit progression reducing ferroptosis within suggesting could serve potential therapeutic myopia.

Language: Английский

Citations

0