Metabolic Characteristics of Sclera in Lens-Induced Myopic Guinea Pigs DOI Creative Commons
Xueqi Lin,

Changyue Zheng,

Jing Cong

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(13), P. 51 - 51

Published: Nov. 25, 2024

Purpose: Myopia development is closely associated with scleral tissue loss in both human and animal models. This research aimed to investigate the metabolic changes sclera of lens-induced myopic guinea pigs explore underlying mechanisms. Methods: was induced 2-week-old pigmented by having them wear −20-diopter lenses for 10 days, one eye designated as other control. Dual-platform untargeted metabolomics performed using gas chromatography–mass spectrometry (GC-MS) liquid (LC-MS) analyze sclera. Validation amino acid levels conducted via targeted metabolomics. Glycine intervention carried out fibroblasts myopia pig model evaluate its effects on COL1A1 synthesis progression. Results: After days myopia, GC-MS LC-MS analyses identified significant 29 85 metabolites sclera, respectively. Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis indicated that downregulation pyrimidine metabolism pathways crucial development. Targeted confirmed multiple acids were significantly reduced deficiency fibroblasts, glycine supplementation increased content. Animal studies demonstrated gavage inhibited axial elongation refractive error increase pigs, content, reversed reduction ferroptosis-related proteins GPX4 FTH1. Conclusions: Several acids, including glycine, l-isoleucine, l-serine, l-valine, along a marked pathways. can content inhibit progression reducing ferroptosis within suggesting could serve potential therapeutic myopia.

Language: Английский

Elevated thiamine level is associated with activating interaction between HIF-1α and SLC19A3 in experimental myopic guinea pigs DOI Creative Commons

Zhongyu Ma,

Jiawen Hao,

Zhaohui Yang

et al.

Frontiers in Medicine, Journal Year: 2025, Volume and Issue: 12

Published: April 25, 2025

Background The SLC19 gene family of solute carriers is a three transporter proteins with similar structures, which SLC19A2 and SLC19A3 mediate thiamin transport; HIF transcriptionally active nuclear protein that key factor activated in hypoxic environments. Myopia the most common eye disease damages visual health adolescents, currently, choroidal hypoxia one prevailing doctrines myopia, as well choroid an ocular nutrient-supporting tissue, thiamine may play role. This study aimed to investigate process changes tissue guinea pigs negative lens-induced myopia (LIM). Methods right eyes LIM group wore −6.0D lenses model experimental myopia. Biological measurements parameters thickness (ChT) were measured after 2, 4, 6 weeks modeling. Real-time fluorescence quantitative PCR Western blot used detect expression SLC19A2, SLC19A3, HIF-1α tissues each pig, ELISA was content tissues, HE staining observe morphological tissues. Immunofluorescence immunohistochemistry detected at different modeling times, immunoprecipitation molecular docking verified interactions between SLC19A3. Results Compared normal control (NC) group, showed significant increase axial length decrease refractive error, thinning loosening structure. In addition, higher than NC 2 4 weeks, weeks. Moreover, revealed reciprocal relationship their sites action. Conclusion current suggests myopic has metabolic abnormalities. Thiamine, critical molecule for metabolism, role process. Our findings indicate levels within are associated elevated activation enhances transport. adaptive regulatory mechanism research highlights potential target pharmacological inhibitors could lead new insights into mechanisms novel strategies treating disease.

Language: Английский

Citations

0
Animal modeling for myopia DOI Creative Commons

Lingman Zheng,

Zhiyong Liao, Jian Zou

et al.

Advances in Ophthalmology Practice and Research, Journal Year: 2024, Volume and Issue: 4(4), P. 173 - 181

Published: June 5, 2024

Myopia is one of the most common eye diseases globally, and has become an increasingly serious health concern among adolescents. Understanding factors contributing to onset myopia strategies slow its progression critical reducing prevalence.

Language: Английский

Citations

2
The potential role of amino acids in myopia: inspiration from metabolomics DOI
Ying Xie, Liyue Zhang,

Si‐Yi Chen

et al.

Metabolomics, Journal Year: 2024, Volume and Issue: 21(1)

Published: Dec. 15, 2024

Language: Английский

Citations

1
Therapeutic Effects of Taurine and Histidine Supplementation in Retinal Diseases DOI Creative Commons
Deokho Lee, Lois E.H. Smith

Life, Journal Year: 2024, Volume and Issue: 14(12), P. 1566 - 1566

Published: Nov. 29, 2024

Amino acids are basic building blocks of structural proteins and enzymes. They also act as signaling molecules fuel. characterized essential if sufficient quantities must be supplied exogenously or non-essential they can endogenously synthesized. Appropriate intake amino not only prevents the development metabolic diseases but reduce progression some disease states. strongly associated with retinal metabolism in physiology pathology. Nonetheless, there is a lack robust clinical studies supporting benefits acid supplementation retinopathy. In this review, we summarize preclinical evidence concerning potential supplementing taurine histidine to provide protection against diabetic retinopathy, glaucoma, age-related macular degeneration. We suggest further directions for studying acid-based therapeutic interventions eye diseases.

Language: Английский

Citations

0
Metabolic Characteristics of Sclera in Lens-Induced Myopic Guinea Pigs DOI Creative Commons
Xueqi Lin,

Changyue Zheng,

Jing Cong

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(13), P. 51 - 51

Published: Nov. 25, 2024

Purpose: Myopia development is closely associated with scleral tissue loss in both human and animal models. This research aimed to investigate the metabolic changes sclera of lens-induced myopic guinea pigs explore underlying mechanisms. Methods: was induced 2-week-old pigmented by having them wear −20-diopter lenses for 10 days, one eye designated as other control. Dual-platform untargeted metabolomics performed using gas chromatography–mass spectrometry (GC-MS) liquid (LC-MS) analyze sclera. Validation amino acid levels conducted via targeted metabolomics. Glycine intervention carried out fibroblasts myopia pig model evaluate its effects on COL1A1 synthesis progression. Results: After days myopia, GC-MS LC-MS analyses identified significant 29 85 metabolites sclera, respectively. Kyoto Encyclopedia Genes Genomes (KEGG) pathway analysis indicated that downregulation pyrimidine metabolism pathways crucial development. Targeted confirmed multiple acids were significantly reduced deficiency fibroblasts, glycine supplementation increased content. Animal studies demonstrated gavage inhibited axial elongation refractive error increase pigs, content, reversed reduction ferroptosis-related proteins GPX4 FTH1. Conclusions: Several acids, including glycine, l-isoleucine, l-serine, l-valine, along a marked pathways. can content inhibit progression reducing ferroptosis within suggesting could serve potential therapeutic myopia.

Language: Английский

Citations

0