Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Aug. 18, 2023
Abstract
In
this
study,
two
different
concentrations
of
matrix
certified
reference
materials
(CRMs)
were
produced
for
the
accurate
measurement
aflatoxin
M1(AFM1)
in
milk
powder((GBW(E)
100552,
GBW(E)
100553)).
The
raw
material
was
obtained
by
feeding
cows
with
positive
drugs.
homogeneity,
stability
and
characterization
CRMs
examined
liquid
chromatography
tandem
mass
spectrometry
isotope-labeled
internal
standard
method.
value
low
concentration
AFM1
powder
2.45
µg/kg
an
uncertainty
0.43
(coverage
factor
k
=
2,
at
95%
confidence).
high
3.45
expanded
0.49
addition,
samples
evaluated
detail
long-term
-80°C
6
months,
short-term
4°C
7
days.
results
showed
that
stable
under
above
conditions.
ACS Nano,
Journal Year:
2024,
Volume and Issue:
18(45), P. 31174 - 31187
Published: Nov. 1, 2024
Multiple
and
ultrasensitive
detection
of
pathogenic
bacteria
is
critical
but
remains
a
challenge.
Here,
we
introduce
digital
assay
for
multiplexed
target
DNA
amplification-free
using
botryoidal-like
fluorescent
polystyrene
dots
(PS-dots),
which
were
first
prepared
through
the
hybridization
reaction
between
primer
exchange
chains
nanospheres
that
encapsulated
polymer
signal
preamplification.
The
bacteria's
was
cleavaged
by
argonaute
(Ago)
protein-mediated
multiple
precise
cleavage
reactions,
where
obtained
sequences
bridged
magnetic
beads
(MBs)
PS-dots
via
reaction,
MB-botryoidal
PS-dot
complexes
utilized
as
probes
based
on
colors
sizes
encoding.
An
artificial-intelligence-fluorescent
microsphere
counting
algorithm
applied
to
identify
count
MBs
readout.
This
combined
ultrabright
with
Sustainability,
Journal Year:
2023,
Volume and Issue:
15(13), P. 10413 - 10413
Published: July 1, 2023
Veterinary
drug
residues
of
common
food
(milk,
meat)
have
posed
serious
threats
to
the
environment
and
human
health,
making
quality
safety
agricultural,
livestock,
aquatic
products
increasingly
prominent.
With
widespread
use
veterinary
drugs
requirements
for
safety,
it
has
become
urgent
detect
in
animal-derived
foods.
So
far,
few
studies
systematically
reviewed
progresses,
challenges,
future
directions
residue
detection.
A
thorough
review
on
current
advancements,
potential
detection
will
be
extremely
beneficial
timely.
This
study
recent
developments
technology
residues.
The
issues
challenges
were
examined
highlighted.
Finally,
proposals
prospects
suggested.
High-throughput
high-sensitivity
technology,
sample
pretreatment
rapid
processing,
fusion
multiple
methods
recommended
as
main
development
It
was
suggested
develop
analysis
technologies
towards
high
automation,
sensitivity,
throughput
future.
provides
new
ideas
strategies
animal
husbandry
industry
protecting
consumers’
physical
health
safety.
The Analyst,
Journal Year:
2024,
Volume and Issue:
149(9), P. 2747 - 2755
Published: Jan. 1, 2024
Avian
leukemia
is
an
infectious
tumorous
disease
of
chickens
caused
by
subgroup
A
the
avian
virus
(ALV-A),
which
mainly
causes
long-term
viremia,
slow
growth,
immune
suppression,
decreased
production
performance,
multi-tissue
tumors,
and
even
death.
The
infection
rate
this
very
high
in
chicken
herds
China,
causing
huge
economic
losses
to
poultry
industry
every
year.
We
successfully
expressed
specific
antigen
protein
ALV
(P27)
through
recombinant
technology
screened
a
pair
highly
sensitive
monoclonal
antibodies
(mAbs)
mouse
immunity,
cell
fusion,
antibody
pairing.
Based
on
antibodies,
we
established
dual
sandwich
ELISA
gold
nanoparticle
immunochromatographic
strip
(AuNP-ICS)
detection
method.
In
addition,
parameters
AuNP-ICS
were
optimized
under
different
reaction
conditions,
resulted
minimum
limits
0.2
ng
mL
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(3), P. 183 - 183
Published: March 13, 2025
Food
safety
remains
a
significant
global
challenge
that
affects
human
health.
Various
hazards,
including
microbiological
and
chemical
threats,
can
compromise
food
throughout
the
supply
chain.
To
address
issues
ensure
public
health,
it
is
necessary
to
adopt
rapid,
accurate,
highly
specific
detection
methods.
Immunoassays
are
considered
be
an
effective
method
for
of
sensitive
biochemical
indicators
provide
efficient
platform
identification
hazards.
In
immunoassays,
antibodies
function
as
primary
recognition
elements.
Nanobodies
have
potential
valuable
biomolecules
in
diagnostic
applications.
Their
distinctive
physicochemical
structural
characteristics
make
them
excellent
candidates
development
reliable
assays,
promising
alternatives
monoclonal
polyclonal
antibodies.
Herein,
we
summarize
comprehensive
overview
status
prospects
nanobody-based
immunoassays
ensuring
safety.
First,
begin
with
historical
perspective
on
nanobodies
their
unique
characteristics.
Subsequently,
explore
definitions
boundaries
immunosensors,
before
discussing
applications
testing
emerged
over
past
five
years,
follow
different
highlighting
advantages
traditional
Finally,
directions
challenges
discussed.
Due
remarkable
sensitivity,
specificity
versatility,
hold
great
promise
revolutionizing
health
well-being.
Pathogens,
Journal Year:
2023,
Volume and Issue:
12(12), P. 1441 - 1441
Published: Dec. 13, 2023
Salmonellosis
is
a
disease
transmitted
by
contaminated
food
and
one
of
the
leading
causes
infections
worldwide,
making
early
detection
Salmonella
crucial
importance
for
public
health.
However,
current
methods
are
laborious
time-consuming,
thus
impacting
entire
supply
chain
to
production
losses
economic
sanctions.
To
mitigate
these
issues,
number
different
biosensors
have
been
developed,
including
lateral
flow
assays
(LFAs),
which
emerged
as
valuable
tools
in
pathogen
due
their
portability,
ease
use,
time
efficiency,
cost
effectiveness.
The
performance
LFAs
has
considerably
enhanced
development
new
nanomaterials
over
years.
In
this
review,
we
address
principles
formats
assay
discuss
future
prospects
challenges
with
an
emphasis
on
developed
serovars
food.
