α-Mangostin suppresses ethanol-induced gastric ulceration by regulating the Nrf2/HO-1 and NF-κB/NLRP3/caspase-1 signaling pathways and gut microbiota DOI Creative Commons

Suqin Yang,

Gang Liu,

Xiankun Xia

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(2), P. e24339 - e24339

Published: Jan. 1, 2024

α-Mangostin is a natural xanthone derivative isolated from Camellia atrophy (CA), commonly known as Lichuan black tea (LBT). The present study investigated the ameliorating effect and mechanism of α-mangostin on alcoholic gastric ulcers (GU) in rats. In vivo, relieved pathological symptoms. Moreover, regulated activation nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/heme oxygenase 1 (HO-1) κB (NF-κB)/NLR family pyrin domain containing 3 (NLRP3)/caspase-1 pathways. Reactive oxygen species (ROS), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) were significantly decreased IL-10 increased, microtubule-associated protein light chain (LC3)-II/LC3-I ratio was p62 expression decreased, inducible nitric oxide synthase (iNOS) cyclooxygenase (COX-2) down-regulated. relevant mechanisms validated using GSE-1 RAW264.7 cells an vitro model. Furthermore, increased Ligilactobacillus Muribaculum abundance well propionic acid butyric contents. Therefore, possesses antioxidant anti-inflammatory properties, remodels intestinal flora dysbiosis through that may involve regulation Nrf2/HO-1 pathway NF-κB/NLRP3/caspase-1 pathway. It also increases This provides novel evidence regarding use for treating GU.

Language: Английский

Total flavonoids of Broussonetia papyrifera alleviate non-alcohol fatty liver disease via regulating Nrf2/AMPK/mTOR signaling pathways DOI
Qi Wang, Yunfei Wei, Yeling Wang

et al.

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Journal Year: 2024, Volume and Issue: 1869(5), P. 159497 - 159497

Published: April 21, 2024

Language: Английский

Citations

6
Process optimization and identification of antioxidant peptides from enzymatic hydrolysate of bovine bone extract, a potential source in cultured meat DOI Creative Commons

N. Jabena Begum,

Qudrat Ullah Khan, Sam Al‐Dalali

et al.

Frontiers in Sustainable Food Systems, Journal Year: 2024, Volume and Issue: 7

Published: Feb. 7, 2024

Bone protein is a significant secondary product of the meat industry, comprising substantial quantity protein. These proteins could be broken down through enzymatic hydrolysis to generate antioxidant peptides. This study aimed produce peptides from bovine bone extract by utilizing Flavourzyme and Protamex optimizing enzyme amounts time using Box–Behnken design. The final optimized conditions obtained model were as follows: amount was 1,100 U, 2,814 3.77 (h). Bovine hydrolysate (BBEH) purified stepwise ultrafiltration membranes with molecular cutoffs 5, 3, 1 kDa. To assess capacity fractions, several methods used, including radical scavenging activity “1,1-diphenyl-2-picrylhydrazyl (DPPH),” “2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS),” metal chelating (MCA), reducing power (RP), thiobarbituric acid assay (TBA). results indicated that fraction weight less than kDa showed activity, 48, 42, 50% inhibition rates for DPPH, ABTS, chelating, respectively. Using size exclusion chromatography, further separated into five sub-fractions: Frac-I, Frac-II, Frac-III, Frac-IV, Frac-V. Sub-Frac-III, which exhibits DPPH (55%) 0.8 at 700 nm, six sub-sub-fractions reversed-phase HPLC (RP-HPLC) based on hydrophobicity. sub-sub-fraction highest value sub-Fra-III-6, exhibited approximately 69.45% activity. sub-Fra-III-6 analyzed LC–MS/MS, identified two specific peptides: Ala-Pro-Phe mass 333.12 Da Asp-His-Val 369.14 Da. are likely primary might have crucial role in capacity. It can concluded BBEH valuable source natural antioxidants has potential serve viable resource cultured industry.

Language: Английский

Citations

5
Production, identification, in silico analysis, and cytoprotection on H2O2-induced HUVECs of novel angiotensin-I-converting enzyme inhibitory peptides from Skipjack tuna roes DOI Creative Commons
Wangyu Zhu, Yumei Wang,

Ming-Xue Ge

et al.

Frontiers in Nutrition, Journal Year: 2023, Volume and Issue: 10

Published: July 12, 2023

Background Exceeding 50% tuna catches are regarded as byproducts in the production of cans. Given high amount and their environmental effects induced by disposal elimination, valorization nutritional ingredients from these by-products receives increasing attention. Objective This study was to identify angiotensin-I-converting enzyme (ACE) inhibitory (ACEi) peptides roe hydrolysate Skipjack ( Katsuwonus pelamis ) evaluate protection functions on H 2 O -induced human umbilical vein endothelial cells (HUVECs). Methods Protein roes with ACEi activity prepared using flavourzyme, were isolated ultrafiltration chromatography methods identified ESI/MS Procise Protein/Peptide Sequencer for N-terminal amino acid sequence. The mechanism action investigated through molecular docking cellular experiments. Results Four WGESF (TRP3), IKSW (TRP6), YSHM (TRP9), WSPGF (TRP12), respectively. affinity (TRP12) ACE −8.590, −9.703, −9.325, −8.036 kcal/mol, experiment elucidated that significant ability mostly owed tight bond ACE’s active sites/pockets via hydrophobic interaction, electrostatic force hydrogen bonding. Additionally, could dramatically elevate Nitric Oxide (NO) bring down endothelin-1 (ET-1) secretion HUVECs, but also abolish opposite impact norepinephrine (0.5 μM) NO ET-1. Moreover, lower oxidative damage apoptosis rate indicated they increase content superoxide dismutase (SOD) glutathione peroxidase (GSH-Px) decrease generation reactive oxygen species (ROS) malondialdehyde (MDA). Conclusion beneficial healthy products ameliorating hypertension cardiovascular diseases.

Language: Английский

Citations

11
Process optimization and characterization of hydrolysate from underutilized brown macroalgae (Padina tetrastromatica) after fucoidan extraction through subcritical water hydrolysis DOI
Nidhi Hans, Divyang Solanki,

Tanya Nagpal

et al.

Journal of Environmental Management, Journal Year: 2023, Volume and Issue: 349, P. 119497 - 119497

Published: Nov. 9, 2023

Language: Английский

Citations

11
α-Mangostin suppresses ethanol-induced gastric ulceration by regulating the Nrf2/HO-1 and NF-κB/NLRP3/caspase-1 signaling pathways and gut microbiota DOI Creative Commons

Suqin Yang,

Gang Liu,

Xiankun Xia

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(2), P. e24339 - e24339

Published: Jan. 1, 2024

α-Mangostin is a natural xanthone derivative isolated from Camellia atrophy (CA), commonly known as Lichuan black tea (LBT). The present study investigated the ameliorating effect and mechanism of α-mangostin on alcoholic gastric ulcers (GU) in rats. In vivo, relieved pathological symptoms. Moreover, regulated activation nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/heme oxygenase 1 (HO-1) κB (NF-κB)/NLR family pyrin domain containing 3 (NLRP3)/caspase-1 pathways. Reactive oxygen species (ROS), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) were significantly decreased IL-10 increased, microtubule-associated protein light chain (LC3)-II/LC3-I ratio was p62 expression decreased, inducible nitric oxide synthase (iNOS) cyclooxygenase (COX-2) down-regulated. relevant mechanisms validated using GSE-1 RAW264.7 cells an vitro model. Furthermore, increased Ligilactobacillus Muribaculum abundance well propionic acid butyric contents. Therefore, possesses antioxidant anti-inflammatory properties, remodels intestinal flora dysbiosis through that may involve regulation Nrf2/HO-1 pathway NF-κB/NLRP3/caspase-1 pathway. It also increases This provides novel evidence regarding use for treating GU.

Language: Английский

Citations

4