Biochemical and Phylogenetic Analysis of Italian Phaseolus vulgaris Cultivars as Sources of α-Amylase and α-Glucosidase Inhibitors
Plants,
Journal Year:
2023,
Volume and Issue:
12(16), P. 2918 - 2918
Published: Aug. 11, 2023
Phaseolus
vulgaris
α-amylase
inhibitor
(α-AI)
is
a
protein
that
has
recently
gained
commercial
interest,
as
it
inhibits
mammalian
activity,
reducing
the
absorption
of
dietary
carbohydrates.
Numerous
studies
have
reported
efficacy
preparations
based
on
this
control
glycaemic
peaks
in
type-2
diabetes
patients
and
overweight
subjects.
A
positive
influence
microbiota
regulation
also
been
described.
In
work,
ten
insufficiently
studied
Italian
P.
cultivars
were
screened
for
α-amylase-
α-glucosidase-inhibiting
well
absence
antinutritional
compounds,
such
phytohemagglutinin
(PHA).
All
presented
α-glucosidase-inhibitor
while
α-AI
was
missing
two
them.
Only
Nieddone
cultivar
(ACC177)
had
no
haemagglutination
activity.
addition,
partial
nucleotide
sequence
gene
identified
with
degenerate
hybrid
oligonucleotide
primer
(CODEHOP)
strategy
to
identify
genetic
variability,
possibly
linked
functional
differences,
expression
gene,
phylogenetic
relationships.
Molecular
showed
expressed
all
cultivars,
close
similarity
between
Pisu
Grogu
Fasolu
cultivars'
α-AI-4
isoform
emerged
from
comparison
partially
reconstructed
primary
structures.
Moreover,
mechanistic
models
revealed
interaction
network
connects
enzyme
characterized
by
hotspots
(Asp38
Tyr186),
providing
some
insights
analysis
structure
different
particularly
regarding
structure-activity
relationship.
This
study
can
broaden
knowledge
about
class
proteins,
fuelling
valorisation
agronomic
biodiversity
through
development
legume
cultivars.
Language: Английский
Purification and Characterization of Proteinaceous Thermostable α-Amylase Inhibitor from Sardinian Common Bean Nieddone Cultivar (Phaseolus vulgaris L.)
Plants,
Journal Year:
2024,
Volume and Issue:
13(15), P. 2074 - 2074
Published: July 26, 2024
The
increasing
need
for
new
treatments
obesity
and
diabetes
has
led
to
the
development
of
drugs
food
supplements
that
could
reduce
carbohydrate
absorption.
Many
starch
blockers,
based
on
common
bean
proteinaceous
inhibitors
against
α-amylase
(α-AI),
are
already
present
market.
extraction
purification
inhibitor
from
a
promising
cultivar
Sardinia
(Nieddone)
is
described,
highlighting
unique
value
Nieddone
cultivar,
particularly
its
inhibitory
activity
digestive
enzymes
complete
lack
hemagglutination
effect
human
red
blood
cells.
α-AI
involved
two
chromatographic
steps
(IEC
SEC)
was
essential
revealing
certain
properties
inhibitor.
purified
tetrameric
structure
(α
Language: Английский