Research and development of methods for simultaneous separation and purification of two isomers of conjugated linoleic acid based on enzyme-assisted combined technologies

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106024 - 106024

Published: Jan. 1, 2025

Language: Английский

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Enzyme loading in the support and medium composition during immobilization alter activity, specificity and stability of octyl agarose-immobilized Eversa Transform

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 295, P. 139667 - 139667

Published: Jan. 9, 2025

Language: Английский

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The current research status of immobilized lipase performance and its potential for application in food are developing toward green and healthy direction: A review

Journal of Food Science, Journal Year: 2025, Volume and Issue: 90(2)

Published: Feb. 1, 2025

Abstract Immobilized lipases have received great attention in food, environment, medicine, and other fields due to their easy separation, high stability (temperature, pH), storage properties. After immobilization, lipase transforms from a homogeneous heterogeneous state, making it easier recover the reaction substrate achieve recycling, which is line with concept of green chemistry reduces protein contamination product. There are various materials for enzyme including polysaccharides natural sources, inorganic compounds, carbon nanotubes, metal–organic framework materials, so forth. Magnetic immobilization carriers been widely studied ability separation by adding magnetic field. Its method can be simply divided into two categories: physical action (adsorption, embedding) chemical binding (covalent, cross‐linking). Some studies mainly discuss support methods, applications immobilized food. On this basis, our review also focuses on changes crosslinking agents lipases, different methods promote new trends study proposes prospects research food industry.

Language: Английский

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Development of Biocatalysts Based on Candida Antarctica B Lipase Immobilized Onto Crosslinked Porous Polymers Containing Varied Monomeric Compositions

Journal of Applied Polymer Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

ABSTRACT Crosslinked polymer particles can be used successfully as support for enzyme immobilization. For this reason, the relationship between particle morphology and immobilization parameters is investigated in present manuscript. Particularly, study focuses on preparation of biocatalysts using lipase from Candida antarctica B (Cal‐B) crosslinked polymers made divinylbenzene (DVB) ethylene glycol dimethacrylate (EGDMA). Several monomers were utilized process, including methyl methacrylate (MMA), styrene (Sty), glycidyl (GMA), methacrylic acid (MA), vinylbenzyl chloride (VBC), 2‐vinylpyridine (Vpy). Many these to synthesize first time. The highest yields achieved Sty‐DVB, MA‐DVB, DVB supports, which presented high surface areas pore diameters. Among developed biocatalysts, CalB‐MMA‐DVB, a specific area 320.9 m 2 /g, esterification activity 1410 ± 176 U/g bio −1 . In contrast, manufactured with smaller but containing polar monomers, such Cal‐B‐MA‐EDGMA, Cal‐B‐GMA‐EDGMA, Cal‐B‐Vpy‐DVB, Cal‐B‐VBC‐EDGMA, higher hydrolytic activities (HA). Cal‐B‐VBC‐EDGMA catalytic constant (Kcat) 73.48 × 10 s

Language: Английский

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Sustainable design of hollow macroporous chitin carrier with enhanced loading capacity for high-performance lipase bioreactor

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: 310, P. 143494 - 143494

Published: April 24, 2025

Language: Английский

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Fabrication of immobilized lipases from Candida rugosa on hierarchical mesoporous silica for enzymatic enrichment of ω-3 polyunsaturated fatty acids by selective hydrolysis

Food Chemistry X, Journal Year: 2024, Volume and Issue: 22, P. 101434 - 101434

Published: May 10, 2024

In this study, lipase from

Language: Английский

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Insights on the role of blocking agent on the properties of the lipase from Thermomyces lanuginosus immobilized on heterofunctional support for hydroesterification reactions

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 275, P. 133555 - 133555

Published: July 1, 2024

Here, we report a study of the effect blocking agent on properties lipase from Thermomyces lanuginosus (TLL) immobilized heterofunctional support (Purolite C18-ethylnediamina (EDA)- vinyl sulfone (VS)-TLL-blocking agent) in different reactions. The performance biocatalysts was compared to those standard hydrophobic C18-TLL) and commercial one (TLL-IM). nature (Cys, Gly Asp) altered enzyme features. TLL-IM always gave comparatively worse performance, with its specificity for oil being very Purolite biocatalysts. Under optimized conditions, C18-TLL yielded 97 % hydrolysis conversion after 4 h using water/waste cooking soybean (WCSO) mass ratio 4.3, biocatalyst load 6.5 wt% temperature 44.2 °C (without buffer or emulsification agent). In esterification reactions purified free fatty acids (FFAs) obtained WCSO, best TLL depended utilized alcohol: linear amyl alcohol preferred by C18-EDA-VS-TLL-Gly, while higher activity achieved utilizing isoamyl as nucleophile C18-EDA-VS-TLL-Cys, C18-EDA-VS-TLL-Asp IM-TLL catalysts. All results indicate influence step final

Language: Английский

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Highly efficient enzymatic enrichment of n-3 polyunsaturated fatty acid glycerides via interfacial biocatalysis in Pickering emulsions

Food Chemistry, Journal Year: 2024, Volume and Issue: 470, P. 142683 - 142683

Published: Dec. 28, 2024

Language: Английский

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Fabrication of Aspergillus oryzae lipase-immobilized magnetic Cu3(PO4)2∙3H2O nanoflowers for efficient synthesis of structured phospholipids

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105503 - 105503

Published: Nov. 1, 2024

Language: Английский

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