Integrated Metabolomics and Transcriptomics Analyses Identify Key Amino Acid Metabolic Mechanisms in Lacticaseibacillus paracasei SMN-LBK

Foods, Journal Year: 2025, Volume and Issue: 14(5), P. 730 - 730

Published: Feb. 21, 2025

During lactobacillus fermentation, the types of proteins in fermentation substrate significantly influence characteristics fermented product. Proteins are composed various amino acids. Consequently, investigating metabolic mechanisms key acids during lactic acid bacteria is important for improving their application food industry. In this study, growth Lacticaseibacillus paracasei SMN-LBK was inhibited following glutamate and arginine deficiency (p < 0.05). Genomic analysis vitro addition assays showed that α-ketoglutarate (OXO), as a precursor glutamate, eliminated inhibition Meanwhile, may be linked to glutamate. Metabolomics illustrated deficiencies mainly affected carbohydrate pathways SMN-LBK, especially pentose phosphate pathway, alanine, aspartate metabolism, metabolism. Transcriptomics further identified affecting specifically glycolysis/gluconeogenesis, involving genes such pfkA, gapA, ldh, argG, argE, argH. Elucidating molecular will provide theoretical foundation understanding differential by bacteria.

Language: Английский

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Research progress regarding physiological functions of probiotics and techniques to enhance their stress resistance: a review

Critical Reviews in Food Science and Nutrition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 29

Published: May 5, 2025

Probiotics have received widespread attention for their unique physiological functions, such as promoting digestion and absorption of nutrients, modulating the immune system, inhibiting pathogenic microorganisms. However, probiotics face severe challenges in terms stability viability practical applications, environmental factors like acid, osmotic pressure, temperature, oxidation bile salts, which make it difficult to maintain sufficient numbers when they are delivered target location. Therefore, some technical protection is needed improve adhesion gastrointestinal tract minimize damage probiotic products during processing, transportation handling. We review underlying mechanisms by exert well various strategies enhance tolerance, especially traditional production methods modification genetic pathways. In addition, application tolerogenic fermented foods clinical medicine discussed. Through in-depth analysis potential function tolerance enhancement, not only provide theoretical support probiotics, but also pave way development functional innovative human disease treatment strategies.

Language: Английский

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Use of Directed Lactic Fermentation to Obtain Plant-Based, Upcycled Beverage from Milk Thistle Endosperm

Sustainability, Journal Year: 2024, Volume and Issue: 16(13), P. 5342 - 5342

Published: June 23, 2024

This study aimed to evaluate the possibility of using milk thistle endosperm (MTE) extract with a protein content >2 g/100 mL obtain fermented product, an alternative beverages. Directed lactic fermentation Lacticaseibacillus rhamnosus was carried out. The course process monitored. changes in pH, saturation medium dissolved oxygen, and temperature were measured. values main nutritional parameters, silymarin content, amino acid profile/content, PDCAAS value (Protein Digestibility-Corrected Amino Acid Score) determined before after fermentation. MTE took place four phases, activity L. being most intense between 4 8 h into process. As result fermentation, there total tested medium, suggesting catabolism aspartic acid, arginine, proline, isoleucine via LAB place. Particularly significant increase lysine valine (4.95 4.68 g AA, respectively), which are limiting acids MTE. Although contained approximately 1% silymarin, no presence this flavonolignan complex found extracts.

Language: Английский

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Lacticaseibacillus rhamnosus Probio-M9: Impact on the metabolic profile of brown probiotic-fermented milk beverages during storage

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105477 - 105477

Published: Nov. 1, 2024

Language: Английский

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