Comprehensive GC-MS Characterization and Histochemical Assessment of Various Parts of Three Colchicum Species from Bulgarian Flora

Plants, Journal Year: 2025, Volume and Issue: 14(2), P. 270 - 270

Published: Jan. 18, 2025

This study presents a comprehensive phyto- and histochemical analysis of three Colchicum species: autumnale L., the Balkan endemic bivonae Guss., Bulgarian diampolis Delip. et Česchm. Using gas chromatography-mass spectrometry (GC-MS), 66 metabolites were identified, encompassing free amino, organic, phenolic, fatty acids, sugars, alkaloids, which distributed among various plant parts. Organ-specific metabolic patterns revealed that corms seeds are particularly rich in supporting their roles chemical defense survival during dormancy. Conversely, flowers, leaves, capsules enriched with energy-related phenolic compounds, playing critical reproduction stress tolerance. Histochemical investigations localized alkaloids predominantly endosperm seeds, parenchyma corms, vascular bundles flowers. Notably, C. displayed unique profiles. Moderate acetylcholinesterase inhibitory activity (AChE) was observed across organs. Statistical analyses demonstrated significant interspecies organ-specific differentiation, certain serving as key markers. These findings enhance our understanding composition, organ specialization, potential source new biomolecules these species. They underscore ecological pharmacological importance taxa provide framework for future research into sustainable utilization bioactivities.

Language: Английский

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Anti-atrophic Role of Ononis natrix subsp. hispanica Extract in Skeletal Muscle Atrophy: Pathway-targeted Insights and Experimental Validation

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106000 - 106000

Published: Jan. 1, 2025

Language: Английский

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Small Steps to the Big Picture for Health‐Promoting Applications Through the Use of Chickweed (Stellaria media): In Vitro, In Silico, and Pharmacological Network Approaches

Food Science & Nutrition, Journal Year: 2024, Volume and Issue: 12(11), P. 9295 - 9313

Published: Oct. 3, 2024

ABSTRACT Stellaria media L., also called chickweed, is widespread in all parts of the world. In present study, we investigated biological properties and chemical profiles different extracts (ethyl acetate, ethanol, ethanol/water, water) S. . The were examined using UHPLC/MS/MS technique. Regarding properties, antioxidant as well enzyme‐inhibiting cytotoxic effects demonstrated by vitro methods. To obtain further information about structure‐ability relationship, network pharmacology molecular docking performed. Twelve phenolic compounds identified most them flavonoids (apigenin, kaempferol derivatives, etc.). water extract showed best free radical scavenging activity, while ethanol was active reducing power tests. When inhibiting AChE, ethyl acetate inhibitory effect. has a good effect on HepG2 (cell viability: 33.9% at concentration 100 g/mL). analysis, performed STRING database, included these 45 cancer‐associated targets. hub genes TP53, CDKN2A, PTEN, KRAS, HRAS. acacetin‐ O ‐hexoside‐ ‐deoxyhexoside napigenin‐7‐ ‐hexoside exhibit remarkable binding energies with proteins. Consequently, can be potential raw materials for designing functional formulations pharmaceutical, nutraceutical, cosmeceutical industries.

Language: Английский

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Exploring of Chemical Profile and Biological Activities of Three Ocimum Species From Comoros Islands: A Combination of In Vitro and In Silico Insights

Cell Biochemistry and Function, Journal Year: 2024, Volume and Issue: 42(7)

Published: Oct. 1, 2024

ABSTRACT Ocimum species have a great interest in different traditional medicinal systems. This study examined the chemical composition, antioxidant properties, enzyme inhibitory effects, and antibacterial antifungal activities of aerial parts gratissimum , americanum basilicum from Comoros Islands. The extracts were analyzed using high‐performance liquid chromatography‐mass spectrometry (HPLC‐MS) to determine their composition. Antioxidant activity was assessed 2,2‐Diphenyl‐1‐picrylhydrazyl (DPPH), 2,2′‐Azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) diammonium salt (ABTS), cupric reducing capacity (CUPRAC), ferric power (FRAP), chelating ability, phosphomolybdenum radical scavenging assays. Enzyme against acetylcholinesterase (AChE), butrylcholinesterase (BChE), tyrosinase, amylase, glucosidase evaluated spectrophotometric methods. Antibacterial tested broth microdilution method selected pathogenic microorganisms. enzymes proteins silico methods with biomolecules these plants. In addition, 111 metabolites identified advanced HPLC/MS techniques. most significant number detected compounds derivatives hydroxycinnamic acids, followed by flavonoid glycosides aglycones hydroxybenzoic acids. All three exhibited activities, O. best‐reducing abilities CUPRAC FRAP assays revealed that had potent effect on tyrosinase (48.01 ± 3.89 mg kojic acid equivalent [KAE]/g), amylase (1.08 0.02 mmol acarbose [ACAE]/g). tests demonstrated possess broad‐spectrum activity. Molecular docking results showed remarkable binding energies target proteins. molecular dynamics simulations chicoric MurE Staphylococcus aureus complex as promising drug candidate. These findings support medical nutraceutical uses suggest possibilities for natural functional applications.

Language: Английский

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Exploring the Anticancer Potential of Dianthus orientalis in Pancreatic Cancer: A Molecular and Cellular Study

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106183 - 106183

Published: Feb. 1, 2025

Language: Английский

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Some biological activities of Thalictrum minus (Ranunculaceae)

International Journal of Secondary Metabolite, Journal Year: 2025, Volume and Issue: 12(1), P. 46 - 53

Published: Jan. 19, 2025

Plants are vital natural resources that used in a wide range of applications. Throughout history, these tools have proven to be valuable assets for individuals. We conducted study assess the biological activity aerial portions Thalictrum minus L. In this particular situation, plant's ethanol and methanol extracts were obtained using Soxhlet equipment. The Rel Assay kits measure total antioxidant status (TAS), oxidant (TOS), oxidative stress index (OSI). antiproliferative effectiveness against A549 lung cancer cell line was assessed MTT test. anticholinesterase by measuring activities acetylcholinesterase butyrylcholinesterase. analyzed, their TAS values determined 4.371±0.083 4.027±0.081, respectively. TOS 11.816±0.121 13.580±0.176, respectively, whereas OSI 0.271±0.007 0.337±0.009. extract plant found greater than extract. had an anti-AChE value 58.90±1.41, while 65.11±1.01. Similarly, anti-BChE 72.25±0.79, 85.79±0.68. Consequently, it established has antioxidant, anticancer, properties.

Language: Английский

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Decoding Chemical Profiles, Biological Functions, and Medicinal Properties of Liquidambar orientalis Extracts Through Molecular Modeling and Bioinformatic Methods

Food Frontiers, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

ABSTRACT Liquidambar orientalis , the Anatolian sweetgum tree, is a relict and endemic species in Southwestern Turkey, traditionally used for therapeutic purposes. Our study comprehensively evaluated potential of L. extracts from its aerial parts to maximize bioactive compound extraction using methanol, ethyl acetate, water as solvents. The methanolic extract exhibited highest phenolic (73.04 ± 3.94 mg gallic acid equivalent [GAE]/g) flavonoid content (48.86 0.76 rutin [RE]/g), demonstrating superior antioxidant activity 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) (256.61 1.70 Trolox [TE]/g), 2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonic acid) (ABTS) (308.41 3.14 TE/g), cupric ion–reducing capacity (CUPRAC) (411.13 8.48 TE/g) assays. It also showed significant enzyme inhibition acetylcholinesterase (4.43 0.09 galanthamine [GALAE]/g), tyrosinase (149.16 1.14 kojic [KAE]/g), amylase (0.93 0.02 mmol acarbose [ACAE]/g), glucosidase (1.60 0.01 ACAE/g), suggesting applications neurodegenerative disease management, skincare, diabetes treatment. Furthermore, methanol displayed promising antimicrobial due compounds such chlorogenic methyl‐3‐ O ‐caffeoyl quinate. potent anticancer effects against lung cancer (A549) cells, with reductions cell viability induction autophagy. aqueous remarkable efficacy prostate (PC3) modulating apoptosis markers. Breast cells (MDA‐MB‐231) differential responses, acetate promoting extract–enhancing molecular docking dynamics simulations provided additional evidence supporting key phytochemicals particularly afzelin epigallocatechin, cancer‐related targets bacterial enzymes. Overall, this fills gap understanding inhibitory diverse extracts, highlighting multi‐targeted applications, therapy.

Language: Английский

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Exploring the Utility of Prunus mahaleb Extracts as a Source of Natural Bioactive Compounds for Functional Applications

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(4)

Published: March 28, 2025

ABSTRACT Prunus mahaleb has garnered attention as a potent medicinal agent and functional component. We aimed to detect the chemical composition biological activities of several parts (fruit, leaves, twigs) P. . Biological were assessed for antioxidant properties, enzyme inhibition, mutagenic/antimutagenic effects, antibacterial efficacy. Antioxidant capabilities evaluated using various assays, including DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, metal chelating. The constituents extracts identified quantified HPLC‐ESI‐MS/MS method. effects inhibition examined on some enzymes, AChE, BChE, tyrosinase, amylase, glucosidase. Ames test was used evaluate mutagenic antimutagenic properties plant extracts. Furthermore, broth microdilution assay employed possible against microorganisms. methanol extract twigs showed superior (DPPH: 388.39 mg TE/g; ABTS: 701.50 CUPRAC: 459.05 FRAP: 264.99 TE/g). demonstrated highest tyrosinase inhibitory activity (61.91 KAE/g). A total 40 metabolites, mainly flavonoids, detected through analysis, revealing that ferulic acid, naringenin, herniarin predominant compounds. In test, tested exhibited no potential. antimutagenicity ethyl acetate from leaves particularly efficient frameshift base pair substitution mutations induced by recognized mutagens. metabolic activation system amplified these strong rates ranging 85% 98%. results indicated effectiveness at dosages between 6.25 0.195 mg/mL, effective Sarcina lutea, Bacillus cereus , Candida albicans Staphylococcus aureus Our findings indicate can serve versatile raw material development health‐promoting applications, medicines, cosmeceuticals, nutraceuticals.

Language: Английский

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Study of the effect of dryness and storage on Ceratonia siliqua L. stem extracts and evaluation of their insecticidal activity

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 1, 2025

Abstract Vector-borne diseases continue to transmit many dangerous pathogens humans. After decades of continuous use insecticides, types vectors have shown the ability build resistance them. This has necessitated development more efficient and environmentally friendly alternatives in form bioinsecticides. Plants contain a wide range phytochemicals with specific targeting, rapid biodegradability, environmental sustainability variety medicinal properties, making them valuable source biologicals. Moreover, this led highly effective new drugs. study aimed identify active ingredients Ceratonia siliqua L., gathered from two consecutive fruiting seasons which were then divided into C. fresh (CSF), dry (CSd), old (stored) stem (CSO) extracts . Metabolomics profiling was performed using UPLC/MS multivariate data analysis. The resulted tentative identification 54 secondary metabolites. These compounds included flavonoids, phenolic acids, withanolides, terpenoids, phenylpropanoids, etc. CSd showed highest number identified components followed by CSO CSF. % nearly equal negative ion mode for three while positive it order CSF > CSO. several exposure periods, plant methanol research significant insecticidal activity against mosquito larvae, Cx. pipiens , housefly larvae M. domestica (CSd) demonstrated (100 MO%) (LC 50 = 0.09 0.07 mg/ml) following 24- 48-hour post-treatments at 1.0 mg/ml. (CSF) most on 2.32 1.80 mg/ml), 24 48 h post-treatment 25 mg/ml concentration. Both killing house fly extract. Therefore, may serve as an bio-agent vector-borne infection control.

Language: Английский

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Unlocking New Pharma/Nutraceutical Frontiers With Neuroprotective Properties of Three Hypericum Species: A Study Combination With In Vitro and In Silico Methodologies

Food Science & Nutrition, Journal Year: 2025, Volume and Issue: 13(4)

Published: April 1, 2025

ABSTRACT This study investigates the phytochemical composition and biopharmacological potential of three Hypericum species ( H. scabrum , lysimachioides uniglandulosum ) from Turkey. Aqueous hydroalcoholic extracts were analyzed for their total phenolic content (TPC), flavonoid (TFC), individual components (by UHPLC–HRMS technique). Antioxidant activities investigated by DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, metal chelating assays. The inhibition effects tested on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase, amylase, glucosidase examined. One hundred compounds identified in chemical composition, specific genus such as hyperoside, hypericin, pseudohypericin, detected. highest TPC was detected ethanol/water extract with 69.21 mg GAE/g. Furthermore, showed strongest free radical reducing effect. more active than water extracts. Neuroprotective assessments indicated downregulation COX‐2 NOS‐2 genes LPS‐stimulated mouse cortex models, alongside modulation SERT NET expression, suggesting reduced neuroinflammation enhanced neurotransmitter release. Molecular docking dynamics analyses highlighted strong binding interactions, especially NET_hyperoside NET_myricitrin complexes. results indicate significant therapeutic these extracts, supporting development natural agents against oxidative stress, neuroinflammation, related neurodegenerative diseases.

Language: Английский

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