Molecular Biotechnology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 24, 2025
Abstract
Kānuka
(
Kunzea
ericoides
)
has
been
traditionally
recognised
for
its
health-promoting
properties.
However,
limited
comprehensive
data
on
bioactive
profile
hinders
potential
industrial
applications.
Therefore,
this
study
investigated
the
constituents
of
kānuka
subcritical
water
extract,
exhibiting
maximum
antioxidant
potential,
using
LC–MS/MS
QTOF.
Experiments
were
performed
at
different
extraction
temperatures
and
times
to
determine
content
capacity.
The
statistically
analysed
select
extracts
with
response
screening.
highest
recovery
polyphenolics
flavonoids
occurred
170
°C,
5
min
(319.96
±
22.67
mg
GAE/g
dw
163.57
9.7
QE/g
dw),
correlating
increased
activity
(DPPH:
87.77
1.74%;
ABTS:
97.62
0.12;
FRAP:
166.43
25.54
TE/g
dw).
Extracts
obtained
180
200
220
°C
shorter
(5,
10,
15
min)
selected
screening
multivariate
principal
component
analysis.
Consequently,
a
rich
composition
phenylpropanoids,
including
flavonoids,
phenolic
acids,
stilbenes,
monolignols,
was
identified
in
Additionally,
bioactives
previously
not
found
identified,
2ʹ,6ʹ-Dihydroxy-4ʹ-methoxydihydrochalcone,
resveratrol,
scopoletin,
naringin,
underscoring
as
source
valuable
metabolites
pharmaceutical
food
industries.
Antioxidants,
Journal Year:
2025,
Volume and Issue:
14(4), P. 400 - 400
Published: March 27, 2025
Bergamot
[Citrus
×
limon
(L.)
Osbeck,
syn.
C.
bergamia
(Risso)
Risso
&
Poit.]
is
primarily
cultivated
in
the
Calabria
region
of
Italy
and
exploited
food
perfumery
industry.
The
epicarp
its
fruit
a
rich
source
essential
oil
(BEO)
containing
mainly
monoterpenes,
which
are
known
for
their
diverse
biological
activities,
including
antimicrobial,
anti-inflammatory,
antiproliferative,
neuromodulatory
effects.
Emerging
evidence
suggests
that
oxidative
stress
plays
central
role
pathogenesis
neurodegenerative
diseases,
particularly
Alzheimer’s
disease
(AD),
where
it
contributes
to
neuronal
dysfunction
cell
death.
Moreover,
heavy
metal
exposure
has
been
identified
as
key
environmental
factor
exacerbating
neurodegeneration
AD.
This
study
aimed
explore
whether
BEO
could
mitigate
(Cd2+,
Hg2+,
Pb2+)-induced
neurotoxicity
SH-SY5Y
cells,
model
system
brain
cells.
MTT
calcein-AM
assays
were
performed
examine
viability
cells
after
each
itself,
or
combination
with
BEO,
whereas
LDH
assay
was
carried
out
determine
effects
towards
necrotic
death
induced
by
metals.
Furthermore,
DCFH-DA
protect
from
metal-induced
stress.
also
investigated
antibacterial
properties
on
different
Gram-positive
Gram-negative
bacterial
strains
belonging
ATCC
collection.
These
results
suggest
may
help
counteract
damage,
Cd2+
toxicity,
potentially
reducing
one
risk
factors
associated
Additionally,
antimicrobial
reinforce
relevance
preventing
infections
contribute
neuroinflammation
