Structure–Antioxidant Activity Relationship of Polysaccharides Isolated by Microwave/Ultrasonic-Assisted Extraction from Pleurotus ferulae DOI Creative Commons
Hongjin Zhou, Zhongxiong Fan, Yuan Li

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 91 - 91

Published: Jan. 14, 2025

To investigate the structure-antioxidant activity relationship, Pleurotus ferulae polysaccharides were extracted using ultrasonic (U-PFPS) and microwave/ultrasonic-assisted methods (MU-PFPS). Compared to U-PFPS with a molecular weight of 1.566 × 103 kDa, MU-PFPS exhibited lower 89.26 kDa. In addition, unlike U-PFPS, which is primarily composed glucose (Glu:Man:Gal = 91.1:3.5:5.4), has more balanced composition Glu:Man:Gal in ratio 39.4:27.8:32.8 contains branched chains. Furthermore, antioxidant analysis revealed that high concentration (at concentrations above 600 μg/mL) demonstrated stronger protective effects against oxidative damage RAW264.7 cells than did. Collectively, these data suggest higher branching degree at appropriate may correlate enhanced enzyme activities. Our work provides method for isolating offers insights into structure-activity relationship polysaccharides, laying foundation future applications polysaccharide modification structural characterization.

Language: Английский

Structure–Antioxidant Activity Relationship of Polysaccharides Isolated by Microwave/Ultrasonic-Assisted Extraction from Pleurotus ferulae DOI Creative Commons
Hongjin Zhou, Zhongxiong Fan, Yuan Li

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(1), P. 91 - 91

Published: Jan. 14, 2025

To investigate the structure-antioxidant activity relationship, Pleurotus ferulae polysaccharides were extracted using ultrasonic (U-PFPS) and microwave/ultrasonic-assisted methods (MU-PFPS). Compared to U-PFPS with a molecular weight of 1.566 × 103 kDa, MU-PFPS exhibited lower 89.26 kDa. In addition, unlike U-PFPS, which is primarily composed glucose (Glu:Man:Gal = 91.1:3.5:5.4), has more balanced composition Glu:Man:Gal in ratio 39.4:27.8:32.8 contains branched chains. Furthermore, antioxidant analysis revealed that high concentration (at concentrations above 600 μg/mL) demonstrated stronger protective effects against oxidative damage RAW264.7 cells than did. Collectively, these data suggest higher branching degree at appropriate may correlate enhanced enzyme activities. Our work provides method for isolating offers insights into structure-activity relationship polysaccharides, laying foundation future applications polysaccharide modification structural characterization.

Language: Английский

Citations

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