Utilizing the Thermostability of Nanozymes for Joule Heating to Remove Background Peroxidase Activities in Lateral Flow Assays DOI
Vasily G. Panferov, Nikita A. Ivanov,

Wenjun Zhang

et al.

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Lateral flow assays (LFAs) are essential for point-of-care testing. The use of peroxidase-mimicking nanozymes as catalytic labels is an actively developing direction in LFA, primarily focused on enhancing sensitivity. However, endogenous peroxidases, naturally present various samples, can interfere with nanozyme signal amplification, leading to a high background and making visual detection more challenging. issue peroxidases particularly significant LFAs wash-free biosensors. In this study, we showcase the remarkable thermostability contrast enzymes, applied analytically relevant lateral aflatoxin B1. By employing Joule heating portable battery-powered device, test strips were rapidly heated 75-80 °C after completing conventional LFA process. This caused thermal denaturation without affecting Au@Pt nanozymes. As result, substrate oxidation strip was carried out solely by nanozymes, which reduced noise improved limit factor 3.5 compared assay heating.

Language: Английский

Utilizing the Thermostability of Nanozymes for Joule Heating to Remove Background Peroxidase Activities in Lateral Flow Assays DOI
Vasily G. Panferov, Nikita A. Ivanov,

Wenjun Zhang

et al.

ACS Sensors, Journal Year: 2025, Volume and Issue: unknown

Published: May 5, 2025

Lateral flow assays (LFAs) are essential for point-of-care testing. The use of peroxidase-mimicking nanozymes as catalytic labels is an actively developing direction in LFA, primarily focused on enhancing sensitivity. However, endogenous peroxidases, naturally present various samples, can interfere with nanozyme signal amplification, leading to a high background and making visual detection more challenging. issue peroxidases particularly significant LFAs wash-free biosensors. In this study, we showcase the remarkable thermostability contrast enzymes, applied analytically relevant lateral aflatoxin B1. By employing Joule heating portable battery-powered device, test strips were rapidly heated 75-80 °C after completing conventional LFA process. This caused thermal denaturation without affecting Au@Pt nanozymes. As result, substrate oxidation strip was carried out solely by nanozymes, which reduced noise improved limit factor 3.5 compared assay heating.

Language: Английский

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