Biosensors,
Journal Year:
2023,
Volume and Issue:
13(5), P. 518 - 518
Published: May 4, 2023
Considering
that
the
strip
method
is
simple
and
convenient
for
users,
a
Europium
nanosphere-based
time-resolved
fluorescent
immunochromatographic
assay
(TRFICA)
rapid
screening
of
4,4′-dinitrocarbanilide
(DNC)
was
developed
to
improve
performance
assays.
After
optimization,
TRFICA
showed
IC50,
limit
detection,
cut-off
values
0.4,
0.07,
5.0
ng
mL−1,
respectively.
No
significant
cross-reactivity
(CR
<
0.1%)
with
15
DNC
analogs
observed
in
method.
validated
detection
spiked
chicken
homogenates,
recoveries
ranged
from
77.3%
92.7%,
coefficients
variation
<14.9%.
Moreover,
time
needed
procedure,
including
sample
pre-treatment,
less
than
30
min
TRFICA,
which
had
never
been
achieved
before
other
immunoassays.
The
newly
test
rapid,
sensitive,
quantitative,
cost-effective
on-site
technique
analysis
muscle.
Toxics,
Journal Year:
2023,
Volume and Issue:
11(8), P. 677 - 677
Published: Aug. 7, 2023
Fipronil,
a
broad-spectrum
insecticide,
is
widely
used
in
agriculture
and
veterinary
practices.
Fipronil-induced
neurotoxicity
potential
adverse
effects
on
humans
aquatic
organisms
have
raised
health
concerns.
Monitoring
programs
been
implemented
globally
to
assess
fipronil
residues
food,
including
fruits,
vegetables,
animal
products.
However,
previous
exposure
assessments
often
focused
specific
food
categories
or
subsets
of
items,
resulting
limited
insights
into
the
overall
risks.
Additionally,
large
number
non-detect
has
introduced
uncertainties
assessment.
To
address
these
issues,
probabilistic
assessment
dose-response
analysis
were
adopted
this
study,
considering
sample
distribution
below
detection
limit
better
characterize
population
variability
risk
assessments.
The
estimated
general
public
ranges
from
6.38
×
10−6
±
0.00017
mg/kg/day
9.83
0.00034
mg/kg/day.
Only
one
out
200,000
simulated
individuals
had
dose
exceeding
reference
(0.048
mg/kg/day,
pRfD),
which
aims
protect
99%
with
less
than
10%
extra
risk.
By
incorporating
data,
more
comprehensive
understanding
risks
associated
Taiwanese
achieved.
In
this
study,
we
compared
a
wide
range
of
cell-based
bioassays
to
the
use
chemical
analysis
followed
by
exposure-activity
ratio
(EAR)
and
Toxicological
Prioritization
index
(ToxPi)
for
prioritizing
chemicals,
sites,
hazard
concerns
in
water
samples.
Surface
samples
were
collected
from
nine
sites
three
Central
Pennsylvania
streams
analyzed
targeted
list
compounds
emerging
concern
(CECs),
including
pesticides,
personal
care
products,
pharmaceuticals.
Cell-based
reporter
assays
evaluated
human
zebrafish
molecular
initiating
events
(MIEs)
endocrine
metabolic
disruption,
altered
lipid
metabolism,
oxidative
stress.
Bioassays
showed
that
29
out
40
had
at
least
one
site
with
activity
over
limit
detection.
The
receptors
exhibited
highest
number
above
effect-based
trigger
(EBT)
values
would
be
expected
cause
toxicity
Aryl
hydrocarbon
receptor
(AhR,
zebrafish),
Pregnane
X
Receptor
(PXR),
Estrogen
Receptor-beta
(ERB),
Androgen
(AR).
Characterizing
collection
their
bioactivity
aligned
closely
stream
which
collected,
Warriors
Mark
Run,
Halfmoon,
Spruce
Run
more
similar
than
other
streams.
sum
all
EARs
each
indicated
pharmaceutical
Carbamazepine
pesticides
Carbaryl
Atrazine
posed
greatest
concern.
However,
predicted
prioritization
based
on
individual
calculated
EAR
different
those
measured
bioassay,
indicating
biologically
active
chemicals
are
present
not
included
analytes.
Taken
together,
these
data
show
beneficial
whereas
mechanism-based
inclusive
known
as
well
unknown
contaminants
thus
overall
quality
prioritization.
