Treatment With Full‐Spectrum Cannabidiol Oil Improved the Pathological Findings of Dystrophic Mutant Mice

Muscle & Nerve, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

ABSTRACT Introduction/Aims Duchenne muscular dystrophy (DMD) is caused by pathogenic variants in the DMD gene, making muscle fibers susceptible to contraction‐induced membrane damage. Given potential beneficial action of cannabidiol (CBD), we evaluated vitro effect full‐spectrum CBD oil on viability dystrophic and vivo myopathy mdx mouse, a model. Methods In vitro, cells from mouse were treated with assessed cell cytotoxic analyses. vivo, fourteen‐day‐old mice received 10 mg/kg/day for 14 days. We analyzed creatine kinase (CK) levels, liver damage markers, histopathology diaphragm (DIA) quadriceps (QUA [myonecrotic positive IgG staining, regenerated fibers/central nuclei, minimum Feret's diameter, fibrosis area, inflammatory presence macrophages, NF‐kappa B content]). Results treatment showed dose‐dependent effect; however, mg/kg was safe effectively improved histopathological assessment parameters DIA QUA: reduction central nuclei: 1.7% ± 2.0% versus 22.4% 5.3% 11.1% 10.7% 32.3% 4.6%; IgG+ myofibers: 0.6% 0.7% 8.4% 1.6% 0.9% 0.3% 7.5% 1.0%; increase myofiber size: 85.2 3.2 64.3 4.0 μm 106.5 8.6 81.2 4.8 μm; decrease area: 6.2% 2.7% 15.1% 2.6% 5.3 4.1 17.3% 2.8%; reduced macrophage 0.05% 0.1% 10.8% 4.3% 1.0% 10.3% 4.9%; NF‐κB levels: 0.2% 5.2% 2.1%; fibrosis: 5.6% 1.8% 12.0% 3.7% 1.3% 0.5% 4.7% 1.5%. It also serum CK. Discussion Full‐spectrum may represent promising new approach treating DMD, but its toxicity must be considered.

Language: Английский

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Cannabinoids: Therapeutic Perspectives for Management of Orofacial Pain, Oral Inflammation and Bone Healing—A Systematic Review

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3766 - 3766

Published: April 16, 2025

Cannabinoids, particularly cannabidiol (CBD) and tetrahydrocannabinol (THC), have been increasingly studied for their therapeutic applications in various medical fields. This systematic review aims to explore role oral surgery, focusing on pain management, inflammation control, bone regeneration. A was conducted using the PRISMA framework identify relevant studies from PubMed, Scopus, Web of Science databases published up November 2024. The included clinical preclinical investigating effects cannabinoids orofacial pain, inflammation, healing. Data study design, cannabinoid types, outcomes were extracted analyzed. CBD most commonly compound, with other evaluating CB1/CB2 receptor agonists, THC, cannabis smoke. Clinical trials showed mixed results: some found effective reducing dental or myofascial while others limited non-superior compared standard treatments (e.g., NSAIDs, corticosteroids). Among four RCTs, three had a low risk bias, one moderate; all nine animal high bias. conclusion, suggest that represent promising non-opioid alternative management inflammation. Although evidence suggests potential benefits cannabinoids, CBD, health contexts, findings are derived heterogeneous studies—many More high-quality, standardized necessary before recommending routine practice.

Language: Английский

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DNA damage and cell death in human oral squamous cell carcinoma cells: the potential biological effects of cannabidiol

Archives of Oral Biology, Journal Year: 2024, Volume and Issue: 169, P. 106110 - 106110

Published: Oct. 16, 2024

Language: Английский

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Characterization of cannabidiol-rich hemp extract containing mucoadhesive gels: Nonaqueous versus aqueous based formulations

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 104, P. 106514 - 106514

Published: Dec. 12, 2024

Language: Английский

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