Genome sequence and evaluation of safety and probiotic potential of Lacticaseibacillus paracasei LC86 and Lacticaseibacillus casei LC89

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 27, 2025

Aim A comprehensive safety assessment of potential probiotic strains was essential for their application in the food industry. This article systematically evaluated characteristics, whole-genome sequence analysis and Lacticaseibacillus paracasei LC86 casei LC89. Methods Firstly, two lactic acid bacteria selected were identified. Secondly, sequencing performed on LC89, antibiotic resistance, pathogenicity, virulence genes analyzed. We tested various properties strains, included tolerance, cell adhesion, hemolytic activity, catalase gelatin hydrolysis, arginine hydrolysis ability, bile salt capacity, mucin degradation, bioamine, D-/L-lactic production susceptibility, to confirm LC89 both vitro vivo . Additionally, we studied acute toxicity mice through a 14-day oral gavage experiment. Results The identified as genomes devoid virulence, resistance pathogenicity genes. exhibited good tolerance temperature, artificial gastric fluid intestinal fluid; they non-hemolytic, all negative. They capability break down proteins demonstrated sensitivity range antibiotics. LD 50 >2 × 10 CFU/kg. Conclusion experimental results above characteristics indicating candidates probiotics human animal applications.

Language: Английский

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Augmenting Cognitive Function in the Elderly with Mild Cognitive Impairment Using Probiotic Lacticaseibacillus rhamnosus CBT-LR5: A 12-Week Randomized, Double-Blind, Parallel-Group Non-Comparative Study

Nutrients, Journal Year: 2025, Volume and Issue: 17(4), P. 691 - 691

Published: Feb. 14, 2025

Probiotics have been shown to enhance cognitive function in individuals with mild impairment (MCI), but their efficacy varies, depending on the strain and dosage. Clinical investigations are crucial confirm safety, efficacy, mechanism of action. This study was designed assess effective dosage, MH-Pro, a test product containing Lacticaseibacillus rhamnosus CBT-LR5 (LR5) skim milk (non-fat dry milk), improving related physiological changes older adults suspected MCI over 12 weeks. In total, 20 participants (mean age: 68.9 years) were randomly assigned 1:1 ratio either low-dose group (1 × 1010 CFU LR5 1622 mg) or high-dose 4055 mg milk) double-blind, parallel-group clinical trial. After weeks, showed significant improvements MOCA-K subdomains, specifically naming (p = 0.01) delayed recall 0.003). Additionally, levels amyloid-β1 40/42 blood significantly decreased 0.03) following supplementation group. The exhibited improvement orientation 0.05). Moreover, overall enhancement observed 0.003), while trend toward 0.06). Fecal analysis revealed bacterial composition, an increase after weeks MH-Pro consumption. Together, these findings provide foundational evidence suggesting that may serve as potential intervention for enhancing through gut-brain axis pathways elderly population. However, given small sample size predominance female participants, impact outcome be limited. Further large-scale studies necessary validate preliminary results. provides recognize use prepare probiotic supplement enhances aging

Language: Английский

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Comprehensive profiling of smoked cheese from raw goat's milk handcrafted in lower Silesia (Poland)

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116398 - 116398

Published: April 1, 2025

Language: Английский

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Evaluation of efficacy and safety of Lacticaseibacillus rhamnosus LRa05 in the eradication of Helicobacter pylori: a randomized, double-blind, placebo-controlled trial

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 21, 2024

Aim This study aims to evaluate the efficacy of Lacticaseibacillus rhamnosus LRa05 supplementation in enhancing Helicobacter pylori ( H. ) eradication rate and alleviating gastrointestinal side effects associated with bismuth quadruple therapy. Methods -positive patients were randomized receive levofloxacin-based therapy combined either probiotic or a placebo for two weeks, followed by (1 × 10 CFU) maltodextrin next weeks. infection was detected 13 C breath test pre- post-treatment. Blood stool samples collected at week 0 4 routine biochemical analysis, serum inflammatory markers. Gastrointestinal symptoms evaluated using symptom rating scale (GSRS). Intestinal microbiota analyzed 16S rRNA sequencing. The research listed under Chinese Clinical Trial Registry (ChiCTR2300072220), written informed consent obtained from all participants. Results group exhibited trend toward higher rates (86.11%) compared (82.86%), though difference not statistically significant. Significant reductions neutrophil count, alanine aminotransferase, aspartate pepsinogen I, interleukin-6 (IL-6), tumor necrosis factor α (TNF-α) p < 0.05) suggest that may mitigate inflammation, enhance liver function, potential aid early cancer prevention. GSRS scores showed alleviated abdominal pain, acid reflux, bloating, diarrhea, patient compliance. Furthermore, sequencing countered antibiotic-induced disruption gut diversity, primarily increasing beneficial bacteria. Conclusion Although did significantly improve success therapy, it has function reduced levels markers such as IL-6 TNF-α body, regulating response. In addition, played positive role adverse disturbances caused providing possible way overall health demonstrating promising clinical potential. Registration http://www.chictr.org.cn , identifier ChiCTR2300072220.

Language: Английский

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Extensive Genomic Characterization, Pre-Clinical Probiotic Evaluation, and Safety Analysis of Bifidobacterium longum subsp. longum BL21 Isolated from Infant Feces

Microbial Pathogenesis, Journal Year: 2024, Volume and Issue: 197, P. 107100 - 107100

Published: Nov. 5, 2024

This study aimed to evaluate the safety and probiotic properties of Bifidobacterium longum subsp. BL21 isolated from infant feces for use as a commercial strain.

Language: Английский

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Lacticaseibacillus rhamnosus LRa05 alleviates cyclophosphamide‐induced immunosuppression and intestinal microbiota disorder in mice

Journal of Food Science, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Abstract Probiotics play a crucial role in regulating the gut microbiota and enhancing immune response. Oral administration of probiotics modulates intestinal composition homeostasis. In this study, we investigated immunoregulatory effect Lacticaseibacillus rhamnosus LRa05 on cyclophosphamide (CTX)‐induced immunosuppressive mice. The results showed that oral reduced weight loss, restored organ indices, maintained structural integrity tissue CTX‐treated Moreover, exhibited immune‐modulating properties by promoting secretion cytokines (tumor necrosis factor‐α, interleukin‐1β, interleukin‐10, secretory immunoglobulin A) serum. analysis 16S rRNA amplicon sequencing revealed increased diversity regulated its composition. detail, intervention Firmicutes/Bacteroidota ratio significantly relative abundance Lachnospiraceae_NK4A136_group , Oscillibacter Alloprevotella Parasutterella Roseburia immunocompromised Conversely, abundances Helicobacter Bacteroides unclassified_Desulfovibrionaceae were decreased after LRa05. Based these findings, orally administered could effectively maintain homeostasis regulate immunity, suggesting potential L. as candidate probiotic strain application dietary supplement. Practical Application Supplement with can improve promote body health.

Language: Английский

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Comprehensive genomic analysis and evaluation of in vivo and in vitro safety of Heyndrickxia coagulans BC99

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 4, 2024

This study aimed to evaluate the safety profile and beneficial effects of Heyndrickxia coagulans strain BC99 (BC99) for potential use in functional foods pharmaceuticals. We began with whole genome sequencing BC99, followed by a comprehensive assessment comprising analysis, hemolysis, cytotoxicity, antibiotic susceptibility tests, cell adhesion tolerance studies, along acute subacute oral toxicity studies animal models. was isolated from well-characterized collection originating feces healthy infant. Our results indicated no hemolytic activity on Columbia blood agar plates broad sensitivity, including gentamicin, ampicillin, chloramphenicol, ciprofloxacin, others. Cytotoxicity testing confirmed adverse HT-29 cells significant adhesive properties intestinal epithelial cells. Tolerance tests demonstrated over 90% viability under simulated gastrointestinal conditions. In vivo mice rats absence following administration. Collectively, these findings support BC99's robust environments, strong capabilities, spectrum resistance, underlining its as safe effective agent gut microbiota modulation host health enhancement.

Language: Английский

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Unveiling Akkermansia muciniphila Akk11: A Comprehensive Genotypic and Phenotypic Safety Assessment

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Background As a beneficial next-generation microorganism, A. muciniphila is beginning to enter the market as commercial product and positioning itself potential candidate for clinical therapy. This study aims evaluate safety probiotic of Akkermansia Akk11 strain isolated from infant feces. Results was identified an anaerobic, Gram-negative with mucin-degrading capabilities, showing up 98.36% genomic similarity type (ATCC BAA-835^T) based on average nucleotide identity (ANI) analysis. Genotypic analysis revealed three putative genes, blaLUS-1 gene encoding class A beta-lactamase, adeF fusion protein belonging resistance-nodule-cell division (RND) antibiotic efflux pump system elongation factor Tu (Ef-Tu) gene, respectively. These genes were found be intrinsic, indicating favorable profile Akk11. Phenotypic tests showed that highly resistant gentamicin, kanamycin streptomycin, while producing no biogenic amines, D-/L-lactic acid or negative hemolytic activity. The good tolerance under gastrointestinal stress conditions, exhibited cytotoxicity strong adhesion intestinal cells in vitro. Furthermore, oral toxicity genotoxicity observed vivo. Conclusion exhibits robust potential, properties comparable those strain. Our research highlights novel strain, Akk11, which provides promising new applications warrants further development.

Language: Английский

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