Biomedicine & Pharmacotherapy,
Journal Year:
2024,
Volume and Issue:
176, P. 116921 - 116921
Published: June 12, 2024
Pulp
therapy
has
been
emerged
as
a
one
of
the
efficient
therapies
in
field
endodontics.
Among
different
types
new
endodontic
materials,
pulpotec
materialized
recognized
material
for
vital
pulp
therapy.
However,
its
efficacy
challenged
due
to
lack
information
about
cellular
biocompatibility.
This
study
evaluates
mechanistic
biocompatibility
cement
with
macrophage
cells
(RAW
264.7)
at
and
molecular
level.
The
was
evaluated
using
experimental
computational
techniques
like
MTT
assay,
oxidative
stress
analysis
apoptosis
through
flow
cytometry
fluorescent
microscopy.
results
showed
concentration-dependent
cytotoxicity
extract
RAW
264.7
an
LC
50
X/10-X/20.
depicted
interaction
components
metabolic
proteins
Sod1
p53.
revealed
effects
Pulpotec
cement's
extract,
showing
induction
apoptosis.
These
were
influential
structural
functional
abnormalities
p53
proteins,
caused
by
their
internalized
cement.
provided
detailed
view
on
utility
applications,
highlighting
biomedical
aspects.
Journal of Xenobiotics,
Journal Year:
2024,
Volume and Issue:
14(3), P. 1201 - 1220
Published: Sept. 4, 2024
Bisphenols
are
a
group
of
endocrine-disrupting
chemicals
used
worldwide
for
the
production
plastics
and
resins.
Bisphenol
A
(BPA),
main
bisphenol,
exhibits
many
unwanted
effects.
BPA
has,
currently,
been
replaced
with
bisphenol
F
(BPF)
S
(BPS)
in
applications
hope
that
these
molecules
have
lesser
effect
on
metabolism
than
BPA.
Since
bisphenols
tend
to
partition
into
lipid
phase,
their
place
choice
would
be
cellular
membrane.
In
this
paper,
I
carried
out
molecular
dynamics
simulations
compare
localization
interactions
BPA,
BPF,
BPS
complex
This
study
suggests
placed
at
membrane
interface,
they
no
preferred
orientation
inside
membrane,
can
monomer
or
aggregated
state,
affect
biophysical
properties
lipids.
The
attributed,
least
part,
membranotropic
effects
modulation
properties.
data
support
both
BPF
BPS,
behaving
same
way
as
capacity
accumulate
biological
not
safe
alternatives
Biomedicine & Pharmacotherapy,
Journal Year:
2024,
Volume and Issue:
176, P. 116921 - 116921
Published: June 12, 2024
Pulp
therapy
has
been
emerged
as
a
one
of
the
efficient
therapies
in
field
endodontics.
Among
different
types
new
endodontic
materials,
pulpotec
materialized
recognized
material
for
vital
pulp
therapy.
However,
its
efficacy
challenged
due
to
lack
information
about
cellular
biocompatibility.
This
study
evaluates
mechanistic
biocompatibility
cement
with
macrophage
cells
(RAW
264.7)
at
and
molecular
level.
The
was
evaluated
using
experimental
computational
techniques
like
MTT
assay,
oxidative
stress
analysis
apoptosis
through
flow
cytometry
fluorescent
microscopy.
results
showed
concentration-dependent
cytotoxicity
extract
RAW
264.7
an
LC
50
X/10-X/20.
depicted
interaction
components
metabolic
proteins
Sod1
p53.
revealed
effects
Pulpotec
cement's
extract,
showing
induction
apoptosis.
These
were
influential
structural
functional
abnormalities
p53
proteins,
caused
by
their
internalized
cement.
provided
detailed
view
on
utility
applications,
highlighting
biomedical
aspects.
