Ferroptosis-Related Genes as Molecular Markers in Bovine Mammary Epithelial Cells Challenged with Staphylococcus aureus DOI Open Access
Xing Yue, Siyuan Mi,

Gerile Dari

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2506 - 2506

Published: March 11, 2025

Staphylococcus aureus-induced mastitis is a significant cause of economic losses in the dairy industry, yet its molecular mechanisms remain poorly defined. Although ferroptosis, regulated cell death process, associated with inflammatory diseases, role bovine unknown. In this study, 11 S. aureus strains were isolated from milk samples obtained cows clinical or subclinical mastitis. Transcriptome analysis Mac-T cells challenged identified differentially expressed genes (DEGs). Enrichment revealed associations between DEG clusters and traits related to KEGG pathway enrichment Toll-like receptor, TNF signaling as significantly enriched pathways. Weighted gene co-expression network (WGCNA) further prioritized ferroptosis-related (HMOX1, SLC11A2, STEAP3, SAT1, VDAC2) involved iron metabolism. Notably, expression levels HMOX1 SAT1 increased aureus-challenged cells, upregulation was consistent trends observed transcriptome data mother–daughter pairs caused by infection. Furthermore, Ferrostatin-1 treatment reduced confirming involvement ferroptosis process. This study reveals that plays key highlights potential target for breeding strategies aimed at improving resistance.

Language: Английский

Ferroptosis-Related Genes as Molecular Markers in Bovine Mammary Epithelial Cells Challenged with Staphylococcus aureus DOI Open Access
Xing Yue, Siyuan Mi,

Gerile Dari

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2506 - 2506

Published: March 11, 2025

Staphylococcus aureus-induced mastitis is a significant cause of economic losses in the dairy industry, yet its molecular mechanisms remain poorly defined. Although ferroptosis, regulated cell death process, associated with inflammatory diseases, role bovine unknown. In this study, 11 S. aureus strains were isolated from milk samples obtained cows clinical or subclinical mastitis. Transcriptome analysis Mac-T cells challenged identified differentially expressed genes (DEGs). Enrichment revealed associations between DEG clusters and traits related to KEGG pathway enrichment Toll-like receptor, TNF signaling as significantly enriched pathways. Weighted gene co-expression network (WGCNA) further prioritized ferroptosis-related (HMOX1, SLC11A2, STEAP3, SAT1, VDAC2) involved iron metabolism. Notably, expression levels HMOX1 SAT1 increased aureus-challenged cells, upregulation was consistent trends observed transcriptome data mother–daughter pairs caused by infection. Furthermore, Ferrostatin-1 treatment reduced confirming involvement ferroptosis process. This study reveals that plays key highlights potential target for breeding strategies aimed at improving resistance.

Language: Английский

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