Cell-end marker proteins are required for hyphal ring formation and size determination of traps in Arthrobotrys flagrans DOI
Marius Kriegler, Valentin Wernet, Birgit Hetzer

et al.

Journal of Cell Science, Journal Year: 2025, Volume and Issue: 138(8)

Published: April 15, 2025

ABSTRACT Filamentous fungi grow by apical extension where secretory vesicles are transported long distances microtubules and actin prior to fusion with the cell membrane. Apical, membrane-bound cell-end marker proteins (CEMPs) organise cytoskeletons thereby growth machinery. CEMPs have been characterised mainly in Schizosaccharomyces pombe Aspergillus nidulans. Here, we studied role of nematode-trapping fungus Arthrobotrys flagrans. This predatory forms ring-shaped adhesive traps capture nematodes, such as Caenorhabditis elegans. Traps morphologically physiologically different from vegetative hyphae generated hyphal turning trap tip basal hypha. The absence membrane-anchored CEMP receptor protein, TeaR, caused a reduction ring size, whereas deletion teaA or teaC largely prevented formation hyphae, most appeared sticks. Hence, compared nidulans, loss function results severe morphological phenotype. mutant strains also show changes cell-to-cell communication fusion, suggesting novel functions interconnections other signalling processes cell.

Language: Английский

Cell-end marker proteins are required for hyphal ring formation and size determination of traps in Arthrobotrys flagrans DOI
Marius Kriegler, Valentin Wernet, Birgit Hetzer

et al.

Journal of Cell Science, Journal Year: 2025, Volume and Issue: 138(8)

Published: April 15, 2025

ABSTRACT Filamentous fungi grow by apical extension where secretory vesicles are transported long distances microtubules and actin prior to fusion with the cell membrane. Apical, membrane-bound cell-end marker proteins (CEMPs) organise cytoskeletons thereby growth machinery. CEMPs have been characterised mainly in Schizosaccharomyces pombe Aspergillus nidulans. Here, we studied role of nematode-trapping fungus Arthrobotrys flagrans. This predatory forms ring-shaped adhesive traps capture nematodes, such as Caenorhabditis elegans. Traps morphologically physiologically different from vegetative hyphae generated hyphal turning trap tip basal hypha. The absence membrane-anchored CEMP receptor protein, TeaR, caused a reduction ring size, whereas deletion teaA or teaC largely prevented formation hyphae, most appeared sticks. Hence, compared nidulans, loss function results severe morphological phenotype. mutant strains also show changes cell-to-cell communication fusion, suggesting novel functions interconnections other signalling processes cell.

Language: Английский

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