Inner Ear Organoids: Strengths and Limitations
Journal of the Association for Research in Otolaryngology,
Journal Year:
2024,
Volume and Issue:
25(1), P. 5 - 11
Published: Feb. 9, 2024
Abstract
Inner
ear
organoids
derived
from
differentiation
of
human
pluripotent
stem
cells
have
recently
gained
momentum
as
tools
to
study
inner
development
and
developmental
defects.
An
additional
exciting
aspect
about
this
technology
is
represented
by
its
translational
potential,
specifically,
the
use
validate
therapeutics
for
hearing
balance
restoration
on
human/patient-specific
cells.
This
latter
will
be
briefly
discussed
here
including
opportunities
current
limitations.
Language: Английский
Cochlear Implantation Outcomes in Genotyped Subjects with Sensorineural Hearing Loss
Journal of the Association for Research in Otolaryngology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 23, 2025
Abstract
Purpose
Cochlear
implants
(CIs)
are
an
effective
rehabilitation
option
for
individuals
with
severe-to-profound
sensorineural
hearing
loss
(SNHL).
While
genetic
factors
play
a
significant
role
in
SNHL,
the
variability
CI
outcomes
remains
unclear.
This
study
evaluated
short-
and
long-term
large
genotyped
cohort
investigated
correlations
defects
their
cochlear
site-of-lesion.
Methods
retrospective,
single-center,
included
220
subjects
(127
females;
299
ears)
pathogenic
variants
identified
31
different
nuclear
genes
mitochondrial
genes.
Audiological
were
measured
pre-
post-implantation.
site-of-lesion
was
categorized
as
pre-synaptic,
post-synaptic,
or
mitochondrial,
based
on
gene
function
expression.
Multiple
regression
analysis
assessed
influencing
outcomes,
including
age
at
implantation,
SNHL
duration,
aid
(HA)
use,
Results
showed
median
phoneme
score
of
90%,
better
early
implantation
(≤
6
years).
Variability
not
linked
to
site-of-lesion,
but
subject-specific
factors,
such
duration
pre-implantation
HA
experience.
A
model
incorporating
these
explained
19%
total
variance
outcomes.
Poorer
(phoneme
scores
<
70%)
more
common
prolonged
auditory
deprivation
older
implantation.
Conclusion
Genotyped
recipients
demonstrated
excellent
largely
attributed
non-genetic
factors.
These
findings
show
that
is
beneficial
type
most
hereditary
underscore
importance
Language: Английский
Maturation of type I and type II rat vestibular hair cells in vivo and in vitro
Frontiers in Cell and Developmental Biology,
Journal Year:
2024,
Volume and Issue:
12
Published: June 4, 2024
Vestibular
sensory
epithelia
contain
type
I
and
II
hair
cells
(HCI
HCII).
Recent
studies
have
revealed
molecular
markers
for
the
identification
of
these
cells,
but
precise
composition
each
vestibular
epithelium
(saccule,
utricle,
lateral
crista,
anterior
posterior
crista)
their
postnatal
maturation
not
been
described
in
detail.
Moreover,
vitro
methods
to
study
this
are
well
developed.
We
obtained
total
HCI
HCII
counts
adult
rats
studied
from
birth
(P0)
day
28
(P28).
Adult
were
found
comprise
∼65%
expressing
osteopontin
PMCA2,
∼30%
calretinin,
∼4%
SOX2
neither
nor
calretinin.
At
birth,
immature
HCs
express
both
P28
showed
an
almost
adult-like
still
contained
1.3%
HCs.
In
addition,
we
free-floating
3D
cultures
at
P1,
which
formed
a
fluid-filled
cyst,
survival
up
(28
DIV).
These
good
HC
resiliency
maturation.
Using
enriched
medium
initial
4
days,
HCI/calretinin+-HCII
ratio
close
vivo
was
obtained.
suitable
mature
pharmacological,
toxicological
research.
Language: Английский