Dissection of the Potential Activity Materials and Mechanism of Cortex Moutan Based on In Vivo Substances Profiling
Separation Science Plus,
Journal Year:
2025,
Volume and Issue:
8(3)
Published: March 1, 2025
ABSTRACT
This
study
aimed
to
identify
the
main
chemical
components
of
Moutan
Cortex
(MC)
using
Ultra‐High‐performance
liquid
chromatography
and
quadrupole
time‐of‐flight
(TOF)
mass
spectrometry
explore
its
therapeutic
targets
for
hemorrhagic
fever
with
renal
syndrome
complications
through
network
pharmacology.
A
total
28
compounds
were
identified
in
MC,
including
7
gallic
acylglucoses,
10
monoterpenoids,
6
paeonol
compounds,
2
organic
acids,
3
flavonoids.
In
addition,
after
rats
ingested
six
prototype
substances
clearly
rat
plasma.
Using
VENNY
STRING
databases,
161
intersection
between
MC
identified,
key
VACM‐1,
PIK3R1,
PIK3CA,
EGFR,
MAPK1,
TP53,
AKT1.
These
are
involved
PI3K‐AKT
other
signaling
pathways,
influencing
cell
survival,
apoptosis,
inflammatory
responses.
Experimental
validation
showed
that
significantly
reduced
blood
glucose,
creatinine,
urea
nitrogen
levels
model
improved
kidney
function.
Additionally,
qRT‐PCR
analysis
demonstrated
high‐dose
intervention
effectively
decreased
expression
VCAM‐1
IL‐1β,
both
serum
at
mRNA
level
tissue,
indicating
potent
anti‐inflammatory
effects.
findings
suggest
exerts
multifaceted
effects
on
HFRS
multicomponent,
multitarget,
multipathway
mechanisms,
providing
a
theoretical
basis
clinical
application.
Language: Английский
Computational investigation of Plectranthus neochilus essential oil phytochemical interaction with dipeptidyl peptidase 4: a potential avenue for antidiabetic drug discovery
Current Pharmaceutical Analysis,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 1, 2025
Language: Английский