Fermentation,
Journal Year:
2024,
Volume and Issue:
10(11), P. 555 - 555
Published: Oct. 31, 2024
Maize
gluten
feed
is
rich
in
micronutrients
and
serves
as
a
good
source
of
protein
dietary
fiber,
but
also
contains
anti-nutritional
factors.
In
this
study,
fermentation
conditions
for
the
degradation
phytic
acid
water-unextractable
arabinoxylans
maize
using
Bacillus
subtilis
were
optimized.
Key
variables
influencing
process
identified
from
seven
potential
parameters
Plackett–Burman
design.
Three
statistically
significant
factors,
i.e.,
time,
inoculum
dose,
material-to-liquid
ratio
further
optimized
through
central
composite
design
efficiency
was
predicted.
The
accuracy
predicted
model
validated
by
subsequent
experimentation.
optimum
determined
to
be
time
84.5
h,
dose
17.1%,
1:3.4.
Under
these
conditions,
48%
32%
degraded.
Following
fermentation,
activities
protease,
xylanase,
phytase,
cellulase
significantly
increased
(p
<
0.001),
contributing
breakdown
arabinoxylans,
which
improved
dispersibility
index,
vitro
digestibility,
mineral
bioavailability.
These
findings
suggest
that
fermenting
with
practical
effective
approach
reducing
anti-nutrients
enhancing
its
nutritional
quality.
Journal of Agricultural and Food Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 7, 2025
Accurate
detection
of
viable
bacteria
is
crucial
for
evaluating
and
monitoring
the
fermentation
process.
However,
complexity
samples
presents
challenges
to
developing
precise
rapid
tools.
Here,
we
present
a
Cas13a-Csm6
tandem
nuclease
probe
capable
one-pot
microorganisms
during
fermentation,
eliminating
need
nucleic
acid
preamplification.
The
RNA-activated
CRISPR-Cas13a
generates
cleavage
substrates
that
serve
as
activators
CRISPR/Cas
III-A
Csm6
system.
Leveraging
high
specificity
efficient
amplification
capacity
CRISPR
cascade,
this
can
detect
1%
Lactobacillus
Bacillus,
facilitating
bacterial
populations
throughout
fermentation.
This
approach
completes
within
30
min
improves
sensitivity
profiling
by
16-fold
compared
with
using
Cas13
alone.
offers
analytical
tool
on-site
quality
fermented
foods.