Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes DOI Creative Commons
Matthew Bolino, Hatice Duman, İzzet Avcı

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Abstract Advances in food production systems and customer acceptance have led to the commercial launch of dietary proteins produced via modern biotechnological approaches as alternatives traditional agricultural sources. At same time, a deeper understanding how components interact with gut microbiome has highlighted importance nuances underpinning diet-microbiome interactions. Novel distinct post-translational modifications resulting from their respective not been characterized, nor they may differ traditionally counterparts. To address this, we characterized protein composition N -glycome yeast-synthesized whey ingredient isolated commercially available ice cream compared this novel powder isolate derived bovine milk. We found that despite strong similarities composition, significantly differs between these sources, reflecting biosynthetic machinery systems. Further, profile diversity synthetic were lower relative protein, both being predominantly composed β-lactoglobulin. Finally, understand whether differences N- glycome profiles affected human microbiome, tested an vitro fecal fermentation model. two sources generated significant among three microbial compositions, which hypothesize is product -glycan degradation by representative communities. This work highlights need affect bioactivity proteins, impact microbiome.

Language: Английский

Novel Endo-β-N-Acetylglucosaminidases Derived from Human Fecal Samples Selectively Release N-Glycans from Model Glycoproteins DOI Creative Commons
Matthew Bolino,

Nadini Haththotuwe Gamage,

Hatice Duman

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(8), P. 1288 - 1288

Published: April 8, 2025

Three novel endo-β-N-acetylglucosaminidases (AVUL01, BCAC01, and BFIN01) classified as members of the glucoside hydrolase (GH) family 18 were identified from human fecal samples then cloned characterized for their ability to hydrolyze two distinct classes N-glycans. Endo-β-N-acetylglucosaminidases (ENGases) are known hydrolysis chitin N,N′-diacetylchitobiose core N-linked glycans, depending on glycan architecture. N-glycans have shown bioactivity substrates in gut microbiome microbes that encode ENGases, thus demonstrating ecological relevance gut. However, types N-glycan structures, example, oligomannosidic or complex, been enrich different within Novel advances food technology commercialized animal-derived dietary proteins with instead traditionally complex using precision fermentation. This indicates there is an unmet need identify gut-derived ENGases act upon determine whether these alter ecology. AVUL01, BFIN01 all demonstrated activity exclusively RNase B bovine lactoferrin; however, they failed show α-1,3-core fucosylated high-mannose derived fetuin horseradish peroxidase, respectively. These results suggest α-1,3 fucosylation architecture inhibit BFIN01. Furthermore, performed significantly better than resulting a greater amount N-glycans, suggesting certain may possess enhanced specificity kinetics evolutionary strategy compete resources.

Language: Английский

Citations

0
The safety assessment of N- trans -feruloyltyramine using In vitro genotoxicity studies and 90-day toxicity study in rats DOI Creative Commons

Sungwon Lee,

Srinivas Seekallu,

Suresh Babu Venkataramaiah

et al.

Human & Experimental Toxicology, Journal Year: 2025, Volume and Issue: 44

Published: April 1, 2025

Introduction N- trans -feruloyltyramine (NFT) is a bioactive compound present in many plant sources. The purpose of the studies was to investigate adverse effects, if any, NFT produced through precision fermentation. Methods An vitro Ames test performed with using bacterial strains at concentrations up 1580 µg/plate and without S9. micronucleus assay human peripheral blood cells culture, without, metabolic activation three different doses. In subchronic toxicity study, adult Sprague Dawley rats (10/sex/group) were fed diets prepared target doses 0, 5000, 10,000 or 20,000 ppm for 90 days. Results assay, there no NFT-related concentration dependent increases revertant colony numbers any tester strains. statistically significant increase number binucleated micronuclei compared vehicle control. found be non-genotoxic when evaluated assays. 90-day rodent well tolerated, related findings observed dose levels tested. There histopathological changes high group both sexes. Conclusion No effect level determined as 1474 mg/kg body weight/day males 1958 females based on actual intake tested under experimental conditions employed.

Language: Английский

Citations

0
Selective Influence of Hemp Fiber Ingestion on Post-Exercise Gut Permeability: A Metabolomics-Based Analysis DOI Open Access
David C. Nieman, Camila A. Sakaguchi, James C. Williams

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(8), P. 1384 - 1384

Published: April 19, 2025

Objectives: This study investigated the effects of 2-week ingestion hemp fiber (high and low doses) versus placebo bars on gut permeability plasma metabolite shifts during recovery from 2.25 h intensive cycling. Hemp hull powder is a rich source two bioactive compounds, N-trans-caffeoyl tyramine (NCT) N-trans-feruloyl (NFT), with potential health benefits. Methods: The participants included 23 male female cyclists. A three-arm randomized, placebo-controlled, double-blind, crossover design was used supplementation periods washout periods. Supplement provided 20, 5, or 0 g/d powder. Participants engaged in an cycling bout at end each three Five blood samples were collected before after (overnight fasted state), h-, 1.5 3 h-post-exercise. Five-hour urine pre-supplementation post-2.25 ingesting sugar solution containing 5 g lactulose, 100 mg 13C mannitol, 1.9 mannitol 450 mL water. An increase post-exercise lactulose/13C ratio (L:13CM) as primary indicator altered permeability. Other outcome measures muscle damage biomarkers (serum creatine kinase, myoglobin), serum cortisol, complete cell counts, metabolites using untargeted metabolomics. Results: No trial differences found for L:13CM, biomarkers. Orthogonal partial least-squares discriminant analysis (OPLSDA) showed distinct when comparing high- low-dose compared to (R2Y = 0.987 0.995, respectively). Variable Importance Projection (VIP) scores identified several relevant metabolites, including 3-hydroxy-4-methoxybenzoic acid (VIP 1.9), serotonin 1.5), 5-hydroxytryptophan 1.4), 4-methoxycinnamic 1.4). Mummichog significant intake multiple metabolic pathways, alpha-linolenic acid, porphyrin, sphingolipid, arginine proline, tryptophan, bile metabolism. Conclusions: period did not have effect cyclists (2.25 bout) data. On contrary, metabolomics that combination consuming nutrient-rich exercising 135 min increased levels beneficial those derived healthy

Language: Английский

Citations

0
Biocatalytic Synthesis of N-trans-feruloyltyramine Using an Amide Bond Synthetase with an ATP Recycling DOI

Bingshan Zhao,

Jason Micklefield,

Yonghua Wang

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: May 2, 2025

Language: Английский

Citations

0
Safety Evaluation of N- trans -caffeoyltyramine Derived From a Strain of Yarrowia lipolytica Through Precision Fermentation DOI

Sungwon Lee,

Srinivas Seekallu,

Suresh Babu Venkataramaiah

et al.

International Journal of Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

N- trans -caffeoyltyramine (NCT) is a phenolic hydroxycinnamic acid amide naturally present in many plant crop species and associated with immune response development processes. Little research has been done on the potential safety of NCT for use as an ingredient conventional foods beverages. This study assessed derived from caffeic produced using genetically engineered strain Yarrowia lipolytica 3599_7 via precision fermentation through vitro genotoxicity assays 90-day dietary oral toxicity rats. The Ames test was performed bacterial strains highest dose tested 5000.0 μg/plate presence absence S9. mammalian micronucleus conducted human peripheral blood lymphocytes culture exposed to at predetermined concentrations (4-hour 24-hour exposure) not genotoxic evident assay. exhibited no adverse effects up tested, where no-observed-adverse-effect level 1427 1983 mg/kg body weight/day males females, respectively.

Language: Английский

Citations

0
Unveiling the components, physiological functions and emerging trends of hydroxycinnamic acid amides in goji berry (Lycium) DOI
Shichao Wei, Yuying Wang,

Shiyu Zhou

et al.

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116696 - 116696

Published: May 1, 2025

Language: Английский

Citations

0
Proteomic and N-glycomic comparison of synthetic and bovine whey proteins and their effect on human gut microbiomes DOI Creative Commons
Matthew Bolino, Hatice Duman, İzzet Avcı

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 18, 2024

Abstract Advances in food production systems and customer acceptance have led to the commercial launch of dietary proteins produced via modern biotechnological approaches as alternatives traditional agricultural sources. At same time, a deeper understanding how components interact with gut microbiome has highlighted importance nuances underpinning diet-microbiome interactions. Novel distinct post-translational modifications resulting from their respective not been characterized, nor they may differ traditionally counterparts. To address this, we characterized protein composition N -glycome yeast-synthesized whey ingredient isolated commercially available ice cream compared this novel powder isolate derived bovine milk. We found that despite strong similarities composition, significantly differs between these sources, reflecting biosynthetic machinery systems. Further, profile diversity synthetic were lower relative protein, both being predominantly composed β-lactoglobulin. Finally, understand whether differences N- glycome profiles affected human microbiome, tested an vitro fecal fermentation model. two sources generated significant among three microbial compositions, which hypothesize is product -glycan degradation by representative communities. This work highlights need affect bioactivity proteins, impact microbiome.

Language: Английский

Citations

3