L-Aspartic acid pyridinium salts: In silico, synthesis and adjuvant vs antibiotic antibacterial evaluation

Results in Chemistry, Journal Year: 2024, Volume and Issue: 7, P. 101366 - 101366

Published: Jan. 1, 2024

In the present study, a new series of chiral L-aspartic acid pyridinium salts have been designed, guided by molecular docking as antiemetics via TrySR or DNA GryB inhibitors, well an adjuvant to chloramphenicol CAT-III inhibitor continue with antibacterial in vitro study. Newly designed molecules synthesized chemistry route and their characterization was done using NMR & HRMS techniques. Biological evaluation compounds has on Gram-negative bacteria, E. coli The results shown that 3a, 3e, 3i, 3j, 3k 3o antibacterial, addition against resistant chloramphenicol.

Language: Английский

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Tinosporae Radix attenuates acute pharyngitis by regulating glycerophospholipid metabolism and inflammatory responses through PI3K-Akt signaling pathway

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: Nov. 6, 2024

Introduction With the onset of COVID-19 pandemic, incidence and prevalence acute pharyngitis (AP) have increased significantly. Tinosporae Radix (TR) is a vital medication utilized in treatment pharyngeal laryngeal ailments, especially AP. The study endeavors to explore unclear molecular mechanisms TR addressing Methods Network pharmacology metabolomics analyses effect on AP were conducted, apossible pathway was validated both vivo using rat model vitro LPS-induced RAW264.7 cells model, through techniques such as histopathological examinations, immunohistochemical technology, ELISA, RT-qPCR, Western blotting systematically possible underlying inhibition by TR. Results discussion analysis identified several key targets, including PIK3CA, IL6, AKT1, TNF, PTGS2, alongside pivotal signaling pathways IL-17, Hepatitis B, nuclear factor kappa B (NF-κB), Influenza A, PI3K-Akt pathway. Most them are closely associated with inflammation. Then, wide-target showed that downregulated substances within glycerophospholipid metabolic pathway, modulated integrated findings from network underscored role attenuation inflammatory responses. Finally, experiments shown can inhibit factors IL-6, TNF - α, COX-2, downregulate targets PI3K AKT thereby alleviate response Our demonstrated exerts an anti-AP suppression release modulation metabolism via suppressing

Language: Английский

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Chromatography-Based Metabolomics as a Tool in Bioorganic Research of Honey

Metabolites, Journal Year: 2024, Volume and Issue: 14(11), P. 606 - 606

Published: Nov. 8, 2024

This review presents the latest research on chromatography-based metabolomics for bioorganic of honey, considering targeted, suspect, and untargeted involving metabolite profiling fingerprinting. These approaches give an insight into metabolic diversity different honey varieties reveal classes organic compounds in profiles, among which, key metabolites such as biomarkers bioactive can be highlighted. Chromatography-based strategies have significantly impacted aspects research, including primary areas botanical origins, origin traceability, entomological maturity. Through use tools complex data analysis, these contribute to detection, assessment, and/or correlation parameters attributes. Bioorganic is mainly focused phytochemicals their transformation, but chemical changes that occur during stages formation remain a challenge. Furthermore, user- environmentally friendly sample preparation methods technologies well future perspectives role metabolomic characterization are discussed. The objective this summarize contributing onf with emphasis (i) analysis by gas liquid chromatography techniques; (ii) obtained profiles; (iii) accumulation biogenic volatile non-volatile markers; (iv) procedures; (v) software databases; (vi) conclusions perspectives. For present review, literature search strategy was based PRISMA guidelines studies published between 2019 2024. outlines importance potential innovations characterizing unlocking its full potential.

Language: Английский

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Antioxidant Effects and Potential Mechanisms of Citrus reticulata ‘Chachi’ Components: An Integrated Approach of Network Pharmacology and Metabolomics

Foods, Journal Year: 2024, Volume and Issue: 13(24), P. 4018 - 4018

Published: Dec. 12, 2024

Citrus reticulata ‘Chachi’ (CRC), recognized for its considerable edible and medicinal significance, is a valuable source of metabolites beneficial to human health. This research investigates the metabolic distinctions antioxidant properties across four different parts CRC, using multivariate statistical analysis interpret metabolomic data network pharmacology identify potential targets relevant signaling pathways. The results indicate differences in sample, with 1622 showing differential expression, including 816 secondary metabolites, primarily consisting terpenoids (31.02%) flavonoids (25.22%). dried mature citrus peel (CP) section demonstrates highest level total phenolics (6.8 mg/g), followed by pulp without seed (PU) (4.52 (PWS) (4.26 (SE) (2.16 mg/g). Interestingly, targeted high-performance liquid chromatography reveals nobiletin tangeretin CP, whereas PU has hesperidin, narirutin, didymin. Furthermore, all sections CRC exhibit robust vitro assessments (CP > PWS SE). Lastly, uncovered mechanisms CRC. offers deeper insights into development utilization byproducts processing industry.

Language: Английский

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