Investigation of the mechanism of chenodeoxycholic acid in treating acute lung injury through network pharmacology and experimental validation

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 17, 2025

Abstract Network pharmacology and molecular simulation techniques were employed to predict the potential targets signaling pathways of chenodeoxycholic acid in treatment acute lung injury. Subsequently, its therapeutic effects on injury preliminarily validated using animal experiments. The target Chenodeoxycholic was predicted network pharmacology. Key active ingredients core further docking studies. Lipopolysaccharide used establish a mouse model study effect A total 73 for identified, primarily HSP90AA1, STAT3, HSP90AB1, EP300, NFKB1. These influence associated with bile secretion, prostate cancer, receptor activation chemical carcinogenesis. modulate various processes, including steroid metabolism, biosynthesis, intracellular pathways, thus contributing Molecular results indicated that exhibited strong binding affinity targets, energies ranging from −5.6729 −7.4138 kcal/mol. reliability verified by dynamics simulations. Results experiments demonstrated effectively ameliorated pathological tissue mice injury, decreased levels IL-6 TNF-α (P < 0.01), increased IL-10 0.01). mRNA expression MTOR, STAT3 inhibited, while level NR1H4 significantly can improve

Language: Английский

Foodomics uncovers functional volatile and non-volatile metabolite dynamics in Mizuna (Brassica rapa L. var. nipponsinica) at microgreen and baby green stages

Food Bioscience, Journal Year: 2025, Volume and Issue: 66, P. 106258 - 106258

Published: March 1, 2025

Language: Английский

Tiliroside from Lagopsis supina Ameliorates Myocardial Ischemia Injury in Zebrafish by Activating the kdr-Mediated PI3K-Akt and MAPK Signaling Pathways

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(5), P. 2313 - 2313

Published: March 5, 2025

Lagopsis supina (Steph. ex. Willd.) Ikonn.-Gal., an ancient Chinese herbal medicine, is traditionally used to treat blood stasis diseases such as myocardial ischemia (MI). However, its pharmacodynamics substances of the anti-MI effect and their potential mechanisms remain unclear. This study aims elucidate effects L. against MI reveal underlying in zebrafish. LSD fraction was screened out for active from by isoprenaline hydrochloride (ISO)-induced It could increase stroke volume, ejection fraction, ventricular short-axis systolic rate zebrafish model. A total 30 compounds (Nos. 1-30) were isolated identified various chromatographic techniques nuclear magnetic resonance spectroscopy. Among them, six compounds, including three lignin 15, 16, 18) flavonoid glycosides 14, 25, 26), showed noticeable activities, tiliroside (No. 25) more active. Molecular docking indicated that has a strong binding ability with proteins KDR, PI3K, Akt, Erk, p38, Bcl-2, Bax, Caspase3. In end, results RT-qPCR manifested markedly upregulated expression levels genes kdr, pik3cb, akt2, mapk1, mapk11, mapk14, bcl-2b prominently downregulated bax caspase3. According above results, activated kdr-mediated PI3K-Akt MAPK signaling pathways exert activity. These discoveries give scientific basis applying treatment suggest new avenues developing candidate therapy.

Language: Английский