Current Challenges, and Potential Solutions to Increase Acceptance and Long-Term Consumption of Cultured Meat and Edible Insects – A Review

Future Foods, Journal Year: 2025, Volume and Issue: unknown, P. 100544 - 100544

Published: Jan. 1, 2025

Language: Английский

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Mechanical properties and texture profile analysis of beef burgers and plant-based analogues

Journal of Food Engineering, Journal Year: 2024, Volume and Issue: 385, P. 112259 - 112259

Published: Aug. 8, 2024

Cultivated meat, or cultured is lab-grown from animal stem cells, differentiated into muscle and/or fat, to yield meat products. The process more sustainable and ethical than traditional farming, allowing meet growing consumer demand. However, there remains a challenge in replicating the organoleptic properties of commercially available products for cultivated applications. Consequently, this study employs single-cycle uniaxial testing (flexion, tension, compression, cutting) governed by ISO standards, texture profiling analysis, ascertain modulus, strain, hardness, adhesiveness, cohesiveness, springiness, resilience chewiness seven burgers. These were tested both raw cooked, comprise beef (including range contents, fat percentages price points) plant-based analogues. Here, we show that (i) mechanical (flexural, compressive cutting strains) textural (cohesiveness, springiness resilience) reveal clear statistically significant divides between cooked compared burgers; (ii) moreover, hardness results able distinguish high content burgers (over 95%), low (below 81%) alternatives, thus, are best suited characterise burger properties; (iii) exists key target values replicate characteristics farmed burgers, identified first time. findings provide novel insights characterisation may contribute future developments ensure acceptance.

Language: Английский

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Future Trends in Food and Dairy Process Engineering and Business: A Comprehensive Exploration

Food engineering series, Journal Year: 2025, Volume and Issue: unknown, P. 515 - 534

Published: Jan. 1, 2025

Language: Английский

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The Effects of Laxogenin and 5-Alpha-hydroxy-laxogenin on Myotube Formation and Maturation During Cultured Meat Production

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 345 - 345

Published: Jan. 2, 2025

Cultured meat (CM) is derived from the in vitro myogenesis of muscle satellite (stem) cells (MSCs) and offers a promising alternative protein source. However, development cost-effective media formulation that promotes cell growth has yet to be achieved. In this study, laxogenin (LAX) 5-alpha-hydroxy-laxogenin (5HLAX) were computationally screened against myostatin (MSTN), negative regulator mass, because their antioxidant properties dual roles as MSTN inhibitors enhancers regulatory factors. silico analysis showed LXG 5HLXG bound with binding free energies −7.90 −8.50 kcal/mol, respectively. At concentration 10 nM, LAX 5HLAX effectively inhibited mRNA expressions MSTN, promoted myogenesis, enhanced myotube formation maturation. addition, by acting agonists ROS downregulating factors, they exhibited antioxidative effects. This study shows supplementation or at nM CM production improves texture, quality, nutritional value. We believe fills research gap on for maturation, which are important factors large-scale improve product value, efficacy.

Language: Английский

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Recent Advances in Biosensor Technologies for Meat Production Chain

Foods, Journal Year: 2025, Volume and Issue: 14(5), P. 744 - 744

Published: Feb. 22, 2025

Biosensors are innovative and cost-effective analytical devices that integrate biological recognition elements (bioreceptors) with transducers to detect specific substances (biomolecules), providing a high sensitivity specificity for the rapid accurate point-of-care (POC) quantitative detection of selected biomolecules. In meat production chain, their application has gained attention due increasing demand enhanced food safety, quality assurance, fraud detection, regulatory compliance. can foodborne pathogens (Salmonella, Campylobacter, Shiga-toxin-producing E. coli/STEC, L. monocytogenes, etc.), spoilage bacteria indicators, contaminants (pesticides, dioxins, mycotoxins), antibiotics, antimicrobial resistance genes, hormones (growth promoters stress hormones), metabolites (acute-phase proteins as inflammation markers) at different modules along from livestock farming packaging in farm-to-fork (F2F) continuum. By real-time data biosensors enable early interventions, reducing health risks (foodborne outbreaks) associated contaminated meat/meat products or sub-standard products. Recent advancements micro- nanotechnology, microfluidics, wireless communication have further sensitivity, specificity, portability, automation biosensors, making them suitable on-site field applications. The integration blockchain Internet Things (IoT) systems allows acquired management, while artificial intelligence (AI) machine learning (ML) enables processing, analytics, input risk assessment by competent authorities. This promotes transparency traceability within fostering consumer trust industry accountability. Despite biosensors' promising potential, challenges such scalability, reliability complexity matrices, approval still main challenges. review provides broad overview most relevant aspects current state-of-the-art development, challenges, opportunities prospective applications regular use safety monitoring, clarifying perspectives.

Language: Английский

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3D Bioprinting of Cultivated Meat Followed by the Development of a Fine-Tuned YOLO Model for the Detection and Counting of Lipoblasts, Fibroblasts, and Myogenic Cells

Frontiers in Bioscience-Landmark, Journal Year: 2025, Volume and Issue: 30(3)

Published: March 18, 2025

Background: Cultured meat holds significant potential as a pivotal solution for producing safe, sustainable, and high-quality protein to meet the growing demands of global population. However, scaling this technology requires innovative bioengineering approaches integrated with software methods assess growth cell cultures. This study aims develop 3D printing hybrid product subsequently design finely tuned You Only Look Once (YOLO) model detecting counting lipoblasts, fibroblasts, myogenic cells. Methods: Cultures multipotent mesenchymal stem cells (MMSCs) fibroblasts were obtained from domestic rabbit Oryctolagus cuniculus domesticus. Standard protocols employed induce adipogenic differentiation MMSCs. Fibroblasts isolated skin biopsy samples. The process utilized bioinks. engineering approach involved development unique print head into printer. Confocal transmission electron microscopy within construct was performed. A dataset digital images cells, created. Four models based on YOLOv8-seg architecture trained annotated images, implemented in Telegram bot. Results: Stable cultures obtained. 3D-printed tissue constructs composed sodium alginate, sunflower successfully fabricated. printer assembled. confirmed viability construct. Ultrastructural analysis revealed dense intercellular contacts high metabolic activity. resulting replicated organoleptic structural properties natural meat. In IT segment, single-class lipoblasts achieved metrics recall 85%, precision 77%, mean Average Precision at IoU threshold 0.50 (mAP50) 79%, which improved multiclass 92%, mAP50 81%. bot capable different types. Conclusions: achieved. Detailed microscopic demonstrated activity polymerized alginate hydrogel. engineered presents alternative Additionally, neural network models, bot, proved effective monitoring culture identifying types across three As result, we developed four YOLOv8 that outperforms model. all exhibited reduced accuracy high-density cultures, where overlapping led undercounting.

Language: Английский

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Spectroscopy as a tool for process development and control in cultured meat production by analyzing media composition

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Analysis of cultured meat medium composition during cultivation is crucial for efficient and reliable production. Spectroscopic methods, such as near infrared (NIR), Fourier transform (FTIR), Raman fluorescence spectroscopy have been proposed suitable techniques this purpose. Nuclear magnetic resonance (NMR) has also suggested a method detailed analysis in bioprocesses. The current study investigated the use these methods measuring content lactate glucose used cell proliferation seven days. NMR showed great potential following formation consumption, while being able to follow other nutrients metabolites. FTIR performed excellently estimating content, with coefficient determination prediction (R²_pred) 0,92 0.86, respectively, root mean square error (RMSEP) 0,021 0,028 g/L, respectively. Fluorescence followed an R²_pred 0,79 RMSEP 0,035 best model. Models were not good any 0,80 0,05 g/L. models similarly, but satisfactory, ranging from 0,67 0,73 0,065 0,068 models. NIR did perform well or glucose. We conclude that can be in-line possibly glucose, at-line both medium.

Language: Английский

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Novel aptasensor based on polyaniline functionalized carboxylated dobby carbon nanotubes and molybdenum disulfide for endotoxin detection

Talanta, Journal Year: 2024, Volume and Issue: 276, P. 126256 - 126256

Published: May 15, 2024

Language: Английский

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Bioreactors for Cultivated Meat Production

Published: Jan. 1, 2024

Language: Английский

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