May the Nitrosative and Carbonyl Stress Promote Inflammation in Patients with Colorectal Cancer?

Journal of Inflammation Research, Journal Year: 2022, Volume and Issue: Volume 15, P. 4585 - 4600

Published: Aug. 1, 2022

Overproduction of reactive nitrogen species (RNS) causes the nitrosative stress, which plays a vital role in development metabolic, inflammatory, and cancerous diseases. However, carbonyl stress biology colorectal cancer (CRC) is still not well understood. Therefore, this study evaluated protein DNA oxidation/glycoxidation, pro- anti-inflammatory cytokines CRC patients compared with healthy controls.Fifty-five (21 women, 34 men) 55 controls matched for sex age were included experiment. Nitrosative parameters (nitric oxide (NO), peroxynitrite, S-nitrosothiols, nitrotyrosine), oxidation (total thiols) glycoxidation products (kynurenine N-formylkynurenine, dityrosine, Amadori products, amyloid), damage markers (8-hydroxydeoxyguanosine (8-OHdG)), as levels cytokines, measured serum or plasma samples.The NO, nitrotyrosine, total thiols, kynurenine, product, amyloid, 8-OHdG, IL1α, IL1β, IL6, IL10, TNF-α, significantly higher than controls. Oxidation positively correlated pro-inflammatory (IL1α, TNFα) (IL10), indicating that redox damages may promote inflammation patients. Many biomarkers differentiate from individuals high sensitivity specificity.Correlations chosen oxidative (IL10) suggest Thus, our research first point further clinical trials focusing on evaluation diagnostic utility larger group

Language: Английский

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Pre-Operative Assessment of Micronutrients, Amino Acids, Phospholipids and Oxidative Stress in Bariatric Surgery Candidates

Antioxidants, Journal Year: 2022, Volume and Issue: 11(4), P. 774 - 774

Published: April 13, 2022

Obesity has been linked to lower concentrations of fat-soluble micronutrients and higher oxidative stress markers as well an altered metabolism branched chain amino acids phospholipids. In the context morbid obesity, aim this study was investigate whether which extent plasma status micronutrients, acids, phospholipids differs between morbidly obese (n = 23) non-obese patients 13). addition plasma, malondialdehyde, retinol, cholesterol triglycerides were assessed in visceral subcutaneous adipose tissue both groups. Plasma γ-tocopherol significantly (p < 0.011) group while other showed no statistically significant differences Branched-chain (all p 0.008) lysine 0.006) compared control group. Malondialdehyde 0.016) 0.002) Significantly phosphatidylcholine, phosphatidylethanolamine, lyso-phosphatidylethanolamine 0.05) their corresponding ether-linked analogs observed, all reduced participants Pre-operative assessment undergoing bariatric surgery is recommended for early identification who might be at risk develop a severe micronutrient deficiency post-surgery. Assessment BCAAs help differentiate metabolic healthy those with disorders.

Language: Английский

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Establishment of a novel weight reduction model after laparoscopic sleeve gastrectomy based on abdominal fat area

Frontiers in Surgery, Journal Year: 2024, Volume and Issue: 11

Published: May 17, 2024

In light of ongoing research elucidating the intricacies obesity and metabolic syndrome, role abdominal fat (especially visceral fat) has been particularly prominent. Studies have revealed that adipose tissue can accelerate development syndrome by releasing various bioactive compounds hormones, such as lipocalin, leptin interleukin. A retrospective analysis was performed on clinical data 167 patients with obesity. Among them, 105 who satisfied predefined inclusion exclusion criteria were included. The parameters evaluated included total area (TAFA), laboratory indicators anthropometric measurements. Weight reduction quantified through percent weight loss (%TWL) excess (%EWL) postoperatively. Binary logistic regression receiver operating characteristic (ROC) curve employed to identify predictors loss. emphasized an independent predictor %EWL ≥75% ( p &lt; 0.001). Total = 0.033) BMI 0.003) %TWL ≥30%. our cohort, ≥30% at 1 year after surgery closely related BMI. Based these results, we formulated a novel model based factors, exhibiting superior predictive value for excellent

Language: Английский

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Intravenous calcitriol administration improves the liver redox status and attenuates ferroptosis in mice with high-fat diet-induced obesity complicated with sepsis

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 177, P. 116926 - 116926

Published: June 20, 2024

Obesity aggravates ferroptosis, and vitamin D (VD) may inhibit ferroptosis. We hypothesized that weight reduction and/or calcitriol administration have benefits against the sepsis-induced liver redox imbalance ferroptosis in obese mice. Mice were fed a high-fat diet for 11 weeks, then half of mice continued to consume diet, while other transferred low-energy 5 weeks. After feeding respective diets 16 sepsis was induced by cecal ligation puncture (CLP). Septic divided into four experimental groups: OS group, injected with saline; OD calcitriol; WS weight-reduction WD calcitriol. groups euthanized at 12 or 24 h after CLP. Results showed group had highest inflammatory mediators lipid peroxide levels liver. Calcitriol treatment reduced iron content, enhanced glutathione/oxidized glutathione ratio, upregulated nuclear factor erythroid 2-related 2, ferroptosis-suppressing protein 1, solute carrier family 7 member expression levels. Also, mitochondrion-associated nicotinamide adenine dinucleotide phosphate oxidase peroxisome proliferator-activated receptor-γ coactivator hypoxia-inducible factor-1α, heme oxidase-1 increased late phase sepsis. These results not noted group. findings suggest elicits more-balanced status, alleviates enhances mitochondrial biogenesis-associated gene expressions. Weight alone minimal influences on biogenesis

Language: Английский

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Lipid Peroxidation in Obesity: Can Bariatric Surgery Help?

Antioxidants, Journal Year: 2022, Volume and Issue: 11(8), P. 1537 - 1537

Published: Aug. 7, 2022

Obesity and chronic oxidative stress, often being associated with each other in a vicious circle, are important factors of diseases. Although it was usually considered to accompany aging wealth, global trends show the increase obesity among children even Third World countries. Being manifested by an imbalance between energy consumption food intake, is characterized excessive or abnormal fat accumulation, impaired redox homeostasis metabolic changes self-catalyzed lipid peroxidation generating 4-hydroxynonenal, pluripotent bioactive product polyunsaturated fatty acids. Conservative methods targeting produced only modest transient results treatment morbid obesity. Therefore, recent years, surgery, primarily bariatric, became attractive for Since adipose tissue well known as stress organ pronounced endocrine functions, surgery balance improvement entire organism. The source lipids lipid-soluble antioxidants, complex pathophysiology should thus be from aspects personalized integrative biomedicine treat appropriate way.

Language: Английский

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Salivary Redox Biomarkers in Insulin Resistance: Preclinical Studies in an Animal Model

Oxidative Medicine and Cellular Longevity, Journal Year: 2021, Volume and Issue: 2021(1)

Published: Jan. 1, 2021

Insulin resistance (IR) is a condition of impaired tissue response to insulin. Although there are many methods diagnose IR, new biomarkers still being sought for early and noninvasive diagnosis the disease. Of particular interest in laboratory diagnostics saliva collected stress-free, noninvasive, straightforward manner. The purpose study was evaluate diagnostic utility salivary redox preclinical studies an animal model. conducted on 20 male Wistar rats divided into two equal groups: standard diet high-fat (HFD). In all fed HFD, IR confirmed by elevated homeostasis model assessment (HOMA-IR) index. We have shown that responsible depletion enzymatic (↓superoxide dismutase) nonenzymatic (↓ascorbic acid, ↓reduced glutathione (GSH)) antioxidant barrier at both central (serum/plasma) gland (saliva) levels. rats, we also demonstrated significantly higher concentrations protein/lipid oxidation (↑protein carbonyls, ↑4-hydroxynoneal (4-HNE)), glycation (↑advanced end products), nitration (↑3-nitrotyrosine) products blood plasma. Salivary antioxidants oxidative stress generally correlate with their levels, while GSH 4-HNE highest correlation coefficient. body weight BMI indices carbohydrate metabolism (glucose, insulin, HOMA-IR) proinflammatory adipokines (leptin, resistin, TNF-α). These differentiate from healthy controls very high sensitivity (100%) specificity (100%). multivariate regression analysis. Summarizing, can be used assess systemic status intensity stress. may potential progression. There need human clinical trials conditions.

Language: Английский

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Obesity and oxidative stress: Implications for female fertility

Animal Research and One Health, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 13, 2024

Abstract Obesity has reached epidemic proportions in most parts of the world, and it is estimated that 1 billion people globally are trapped an obesity pandemic, which seriously compromised human health. Recently, there been a flood research into as well redox lipid metabolism; however, our understanding pathways specific molecular mechanisms by obesity‐induced oxidative stress affects female reproductive function remains limited. In this review, we discuss how pandemic led to lower fertility. We focus on multiple facets obesity‐mediated dysfunction, including follicular atresia, oocyte maturation, embryo implantation, aging, therapeutic interventions have potential normalize obese females, such targeting mitochondrial metabolism antioxidant pathways.

Language: Английский

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Anthropometric and body composition analysis in obese and non-obese subjects in three major cities in Indonesia: A cross-sectional study

Human Nutrition & Metabolism, Journal Year: 2024, Volume and Issue: 37, P. 200271 - 200271

Published: May 3, 2024

Screening is needed for the early detection of obesity, but if through anthropometric examinations and body composition measurements not correct, then cases obesity will increase it be too late to prevent. The aim this study was find a correlation between parameters such as fat mass in adult non-athlete males females. design cross-sectional included 255 men women from three cities Indonesia. research subjects were 18-60 years old underwent examinations. assessed index (BMI), waist circumference, visceral fat, mass, fat-free (FFMI), normalized FFMI; BMI used comparison. For further analysis, chi-squared test Spearman's used. results show that BMI, had highest difference men-women, obese-non obese cases. Body factor has strong with (r=0.687); additionally, circumference can parameter medium (r=0.46) weak (r=0.348). Waist also (r=0.785). While FFMI showed mass. This found assessment detect who are athletes, besides easy prevention action. Apart that, more accurate based on or circumference.

Language: Английский

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Acetaminophen protein adduct concentrations during therapeutic dosing in patients with class II-III obesity compared to non-obese and overweight patients: a prospective observational gender stratified cohort study

Acta Anaesthesiologica Belgica, Journal Year: 2024, Volume and Issue: 75(Supplement 1), P. 63 - 74

Published: Dec. 1, 2024

Background: Obese patients may need higher doses of acetaminophen (APAP) for adequate analgesia, due to increased total clearance and distribution volume. APAP-induced hepatotoxicity is mainly caused through CYP2E1 pathway. Its activity induced by obesity, potentially endangering the safety profile APAP. Metabolic-dysfunction associated liver disease (MASLD) an important risk factor APAP induced-hepatotoxicity. Objectives: This pilot study aimed at observing analyzing related protein adducts (APAP-cysteine APAP-mercapturate) in obese compared non-obese during therapeutic dosing Study design setting: Interim analysis ongoing prospective observational gender-stratified cohort PK study, conducted Ghent University Hospital. Methods: 35 (BMI>35kg/m²) 18 (18,5kg/m²<BMI< 30kg/m²) undergoing laparoscopy were included. All received intravenously 2g 1g q6h. Plasma concentrations measured predefined timepoints after first fifth dose. Main outcome measures: was indirectly assessed measuring adducts. Linear mixed model used assess correlations between repeated measurements plasma and: age, gender, body weight, lean mass metabolic syndrome. Hepatotoxicity evaluated assessing function markers 1.0 µmol/L threshold Results: No statistically significant interaction observed obesity APAP- cysteine or APAP-mercapturate. noted syndrome these Significant found APAP-Cysteine with sex, mass. Statistically differences bilirubin, prothrombin time (PT), international normalized ratio (INR) 30 hours, though without clinical relevance. Conclusions: Obesity did not have a impact on activity. Liver significantly different patients,

Language: Английский

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The Effect of Antidepressant Treatment on Neurocognitive Functions, Redox and Inflammatory Parameters in the Context of COVID-19

Journal of Clinical Medicine, Journal Year: 2023, Volume and Issue: 12(22), P. 7049 - 7049

Published: Nov. 12, 2023

Inflammation is an important component of the etiopathology depression that uses oxidative and nitrosative stress (O&NS) elevated inflammatory markers. SARS-CoV-2 infection also associated with abnormal processes, which may impair effective treatment in COVID-19 survivors. In presented study, thirty-three hospitalized patients major depressive disorder (MDD) were started on antidepressant treatment, twenty-one re-evaluated after 4–6 weeks. The control group consisted thirty healthy volunteers. All participants underwent neuropsychiatric evaluation, biochemical blood urine analyses. results research demonstrated positive correlations Hamilton Depression Rating Scale (HAM-D) scores serum catalase (CAT) urinary S-Nitrosothiols levels, Beck Inventory (BDI) reduced glutathione (GSH) superoxide dismutase (SOD) levels. Depressed a history prior to had higher nitric oxide (NO) levels lower peroxidase (GPx) group, survivors N-formylkynurenine (NFK). Our suggest has modulating effect O&NS, reduces symptoms improves cognitive functions present study does not indicate clinical response baseline antibody Nevertheless, further this area needed systematize

Language: Английский

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