Integrated proteomics and metabolomics analysis of D-pinitol function during hippocampal damage in streptozocin-induced aging-accelerated mice DOI Creative Commons
Xiaoxia Li, Yuan Gao, Baoying Li

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2023, Volume and Issue: 16

Published: Oct. 30, 2023

Diabetes can cause hippocampal damage and lead to cognitive impairment. Diabetic impairment (DCI) is a chronic complication of diabetes associated with high disability rate; however, its pathogenesis therapeutic targets are unclear. We aimed explore the mechanism during evaluate potential role D-pinitol (DP) in protecting tissue improving dysfunction.DP (150 mg/kg/day) was administered intragastrically streptozocin-induced aging-accelerated mice for 8 weeks. Hippocampal tissues were examined using tandem mass tag (TMT)-based proteomics liquid chromatography-mass spectrometry (LC-MS)/MS-based non-targeted metabolomic analysis. Differentially expressed proteins (DEPs) differentially regulated metabolites (DRMs) screened further analysis, some DEPs verified western blotting.Our results showed that 329 had significantly altered expression untreated diabetic (DM), which restored normal after DP treatment 72 cases. In total, 207 DRMs identified DM group, 32 post-DP treatment. These involved metabolic pathways (purine metabolism, arginine proline histidine metabolism), actin cytoskeleton regulation, oxidative phosphorylation, Rap1-mediated signaling.Our study may help better understand suggest target.

Language: Английский

Gut microbiome-based therapies for alleviating cognitive impairment: state of the field, limitations, and future perspectives DOI
Linli Bu, Chen Wang, Junying Bai

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(3), P. 1116 - 1134

Published: Jan. 1, 2024

Cognitive impairment (CI) is a multifaceted neurological condition that can trigger negative emotions and range of concurrent symptoms, imposing significant public health economic burdens on society.

Language: Английский

Citations

5
Nerolidol rescues hippocampal injury of diabetic rats through inhibiting NLRP3 inflammasome and regulation of MAPK/AKT pathway DOI
Yining Lei,

Manqin Li,

Xinran Liu

et al.

BioFactors, Journal Year: 2024, Volume and Issue: unknown

Published: April 16, 2024

Abstract Despite the observation of diabetes‐induced brain tissue damage and impaired learning memory, underlying mechanism remains elusive, effective, targeted therapeutics are lacking. Notably, NLRP3 inflammasome is highly expressed in hippocampus diabetic individuals. Nerolidol, a naturally occurring compound with anti‐inflammatory antioxidant properties, has been identified as potential therapeutic option for metabolic disorders. However, ameliorative capacity nerolidol on hippocampal injury its remain unclear. Network pharmacology molecular docking was used to predict signaling pathways targets treatment diabetes. Then established rat model using streptozotocin (STZ) combined high‐fat diet administered. Morris water maze assess spatial memory capacity. Hematoxylin eosin Nissl staining detect neuronal hippocampus. Transmission electron microscopy extent mitochondria, endoplasmic reticulum (ER) synapses. Immunofluorescence GFAP, IBA1, expression Western blot apoptosis (Bcl‐2, BAX, Cleaved‐Caspase‐3); synapses (postsynaptic densifying protein 95, SYN1, Synaptophysin); mitochondria (DRP1, OPA1, MFN1, MFN2); ER (GRP78, ATF6, CHOP, caspase‐12); (NLRP3, ASC, caspase‐1); inflammatory cytokines (IL‐18, IL‐1β, TNF‐α); AKT (P‐AKT); mitogen‐activated kinase (MAPK) pathway (P‐ERK, P‐p38, P‐JNK) related expression. showed that nerolidol's possible mechanisms treating diabetes MAPK/AKT effects. Animal experiments demonstrated could improve blood glucose, lipids, rats. Furthermore, synaptic, mitochondrial, ultrastructure rats by potentially affecting ER‐related proteins. Further studies revealed decreased neuroinflammation, factor while also decreasing MAPK enhancing improves cannot be shown cognitive function. In conclusion, our study reveals first time can ameliorate damage, ER, mitochondrial we suggest may inhibit affected AKT. These findings provide new experimental basis use associated disease.

Language: Английский

Citations

3
TJ0113-induced mitophagy in acute liver failure detected by Raman microspectroscopy DOI Creative Commons
Chunlian Huang,

Jiaqi Liao,

Xufeng Cen

et al.

Redox Biology, Journal Year: 2025, Volume and Issue: 83, P. 103654 - 103654

Published: April 30, 2025

Language: Английский

Citations

0
2-Hydroxyisobutyric acid targeted binding to MT-ND3 boosts mitochondrial respiratory chain homeostasis in hippocampus to rescue diabetic cognitive impairment DOI Creative Commons

Minzhen Xie,

Siqi Gu, Yan Liu

et al.

Redox Biology, Journal Year: 2024, Volume and Issue: 79, P. 103446 - 103446

Published: Nov. 29, 2024

Language: Английский

Citations

1
Label‐free Raman spectroscopy for the assessment of liver fibrosis DOI Creative Commons

Huiying Cao,

Chen Ma,

Zhitao Wu

et al.

MedComm – Future Medicine, Journal Year: 2023, Volume and Issue: 2(4)

Published: Dec. 1, 2023

Abstract The enormous incidence and dire consequences of liver fibrosis highlight the need for biologically plausible markers as readouts pathology drug efficacy. classic reference standard diagnosis is histopathological examinations. However, it burdened by time‐consuming steps, subjectivity, static nature information. Raman spectroscopy a rapid, objective, label‐free analytical tool that provides numerous molecular Thus, we utilized to characterize pathological features evaluate efficacy drugs (obeticholic acid [OCA] gigantol [YS30]). Principal component analysis with subsequent linear discriminant could basically discriminate between fibrotic mice healthy controls showed animals OCA or YS30 treatment resembled those vehicle treatment. Moreover, identified several potential biomarkers, including cytochrome C , development based on their “fingerprints.” imaging provided quantitative spatial distribution collagen in situ. In addition, demonstrated role revitalizing mitochondrial function alleviate using spectroscopy. Collectively, succeeded tracking progression, assessing efficacy, yielding significant information useful quantitatively linking assessment mechanism action.

Language: Английский

Citations

1
Rapid Identification of Candida auris by Raman Spectroscopy Combined With Deep Learning DOI Open Access
S. Kiran Koya, Michelle Brusatori,

Sally Yurgelevic

et al.

Journal of Raman Spectroscopy, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 26, 2024

ABSTRACT Candida auris is a multidrug‐resistant yeast that can lead to outbreaks in healthcare facilities, even with strict infection prevention and control measures. detection challenging using standard laboratory methods. Advancements identification methods, such as matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry polymerase chain reaction, have improved detection, though these methodologies be costly impractical resource‐limited settings. This study presents practical, portable, reagentless platform known Counter‐Propagating Gaussian Beam Raman Spectroscopy (CPGB‐RS), integrated deep learning spectral analysis for the rapid accurate of C. . method has shown sensitivity 96% specificity 99% differentiating from other highly prevalent pathogenic species, albicans , glabrata tropicalis The differentiation between species based on unique variations their spectra, influenced by differences cell wall composition (including β‐glucan, chitin, mannoprotein), membrane components (like ergosterol), cellular energy states (mitochondrial cytochromes b c). allows automated molecular screening, generating diagnostic results within 2 min, making it practical clinical applications. Furthermore, this technology potential evaluate effectiveness antifungal agents, which could significantly improve patient outcomes.

Language: Английский

Citations

0
Integrated proteomics and metabolomics analysis of D-pinitol function during hippocampal damage in streptozocin-induced aging-accelerated mice DOI Creative Commons
Xiaoxia Li, Yuan Gao, Baoying Li

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2023, Volume and Issue: 16

Published: Oct. 30, 2023

Diabetes can cause hippocampal damage and lead to cognitive impairment. Diabetic impairment (DCI) is a chronic complication of diabetes associated with high disability rate; however, its pathogenesis therapeutic targets are unclear. We aimed explore the mechanism during evaluate potential role D-pinitol (DP) in protecting tissue improving dysfunction.DP (150 mg/kg/day) was administered intragastrically streptozocin-induced aging-accelerated mice for 8 weeks. Hippocampal tissues were examined using tandem mass tag (TMT)-based proteomics liquid chromatography-mass spectrometry (LC-MS)/MS-based non-targeted metabolomic analysis. Differentially expressed proteins (DEPs) differentially regulated metabolites (DRMs) screened further analysis, some DEPs verified western blotting.Our results showed that 329 had significantly altered expression untreated diabetic (DM), which restored normal after DP treatment 72 cases. In total, 207 DRMs identified DM group, 32 post-DP treatment. These involved metabolic pathways (purine metabolism, arginine proline histidine metabolism), actin cytoskeleton regulation, oxidative phosphorylation, Rap1-mediated signaling.Our study may help better understand suggest target.

Language: Английский

Citations

0