Saikogenin A improves ethanol-induced liver injury by targeting SIRT1 to modulate lipid metabolism DOI Creative Commons
Jiang Ming-zhu, Ying Feng, Jingxian Wang

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Nov. 21, 2024

Chronic alcohol consumption can lead to live disease (ALD). Steatosis is a critical hallmark of ALD, making it an important stage for therapeutic intervention. Saikosaponin A (SSa), compound found in Radix Bupleuri, has previously shown promising hepatoprotective, anti-inflammatory, and antioxidant properties. However, its role ALD remains understudied. We employ cell-based screening models chronic-plus-binge ethanol-fed mouse model investigate the protective mechanisms SSa metabolite Saikogenin (SGA), against ethanol-induced hepatocyte injury. Our RNA-seq analysis mice unveils that primarily acts through mTOR PPAR-α signaling pathways liver. Biophysical assays loss function experiments confirm SGA directly binds modulates activity SIRT1 protein, mitigating cell injury via SIRT1-mTOR-PPAR-α axis. Furthermore, displays survival prolonging advantage compared resveratrol treating ALD. This suggests holds promise as potential agent ameliorates cellular damage likely by binding activating suppressing activity.

Language: Английский

Unraveling the Antioxidant Capacity of Spatholobi caulis in Nonalcoholic Fatty Liver Disease: A Multiscale Network Approach Integrated with Experimental Validation DOI Creative Commons
Su-Jin Bae, Won-Yung Lee,

Seon-Been Bak

et al.

Antioxidants, Journal Year: 2023, Volume and Issue: 12(5), P. 1097 - 1097

Published: May 13, 2023

Nonalcoholic fatty liver disease (NAFLD) is a global health problem that closely associated with obesity and metabolic syndrome. Spatholobi caulis (SC) herbal medicine potential hepatoprotective effects; however, its active compounds underlying mechanisms have not been fully explored. In this study, we combined multiscale network-level approach experimental validation to investigate SC's antioxidant properties their impact on NAFLD. Data collection network construction were performed, key identified through multi-scale analysis. Validation was conducted using in vitro steatotic hepatocyte models vivo high-fat diet-induced NAFLD models. Our findings revealed SC treatment improved by modulating multiple proteins signaling pathways, including AMPK pathways. Subsequent experiments showed reduced lipid accumulation oxidative stress. We also validated effects crosstalk emphasizing role hepatoprotection. predicted procyanidin B2 be an compound of it lipogenesis model. Histological biochemical analyses confirmed ameliorated steatosis inflammation mice. This study presents use introduces novel for identifying validating medicine.

Language: Английский

Citations

3
Saikogenin A improves ethanol-induced liver injury by targeting SIRT1 to modulate lipid metabolism DOI Creative Commons
Yufeng Wang, Jiang Ming-zhu, Ying Feng

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 7, 2024

Abstract Chronic alcohol consumption leads to a spectrum of liver disorders, including steatosis, inflammation, and fibrosis, collectively known as disease (ALD). Steatosis is critical hallmark ALD, making it an important target for therapeutic intervention. Saikosaponin A (SSa), compound found in Radix Bupleuri, has previously shown promising hepatoprotective, anti-inflammatory, antioxidant properties. However, its role ALD remains understudied, with direct regulatory targets mechanisms yet be fully elucidated vivo. Moreover, the instability SSa gastric juice raises questions about efficacy oral solutions. In this study, we employed cell-based screening models chronic-plus-binge ethanol-fed mouse model investigate protective metabolite Saikogenin (SGA), against ethanol-induced hepatocyte injury. Our RNA-seq analysis mice unveiled that primarily acts through mTOR PPAR-α signaling pathways liver. Biophysical assays loss-of-function experiments confirmed SGA directly binds modulates activity SIRT1 protein, mitigating cell injury via SIRT1-mTOR-PPAR-α axis. Furthermore, our liver-specific knockdown demonstrated action proteins affected by solutions mediated SIRT1. Significantly, displayed superior safety profile hepatocytes compared SSa. findings highlight binding protein enhancing activity, thereby regulating hepatic fatty acid oxidation adipogenesis ALD. This suggests holds promise potential agent

Language: Английский

Citations

0
Identifying Herbal Candidates and Active Compounds for Psoriasis Through Multiscale Network Analysis DOI Creative Commons

G.‐W. Kim,

Su-Yeon Lee,

Sae‐Ron Shin

et al.

Current Issues in Molecular Biology, Journal Year: 2024, Volume and Issue: 46(11), P. 11993 - 12011

Published: Oct. 25, 2024

Psoriasis is a chronic inflammatory skin disorder characterized by the hyperproliferation of keratinocytes and immune system dysregulation, with significant needs due to limitations adverse effects current treatments. In this study, we sought discover novel herbal candidates their active compounds for psoriasis leveraging multiscale network analysis. We conducted comprehensive analysis data from 348 medicinal herbs compounds, identifying

Language: Английский

Citations

0
Saikogenin A improves ethanol-induced liver injury by targeting SIRT1 to modulate lipid metabolism DOI Creative Commons
Jiang Ming-zhu, Ying Feng, Jingxian Wang

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Nov. 21, 2024

Chronic alcohol consumption can lead to live disease (ALD). Steatosis is a critical hallmark of ALD, making it an important stage for therapeutic intervention. Saikosaponin A (SSa), compound found in Radix Bupleuri, has previously shown promising hepatoprotective, anti-inflammatory, and antioxidant properties. However, its role ALD remains understudied. We employ cell-based screening models chronic-plus-binge ethanol-fed mouse model investigate the protective mechanisms SSa metabolite Saikogenin (SGA), against ethanol-induced hepatocyte injury. Our RNA-seq analysis mice unveils that primarily acts through mTOR PPAR-α signaling pathways liver. Biophysical assays loss function experiments confirm SGA directly binds modulates activity SIRT1 protein, mitigating cell injury via SIRT1-mTOR-PPAR-α axis. Furthermore, displays survival prolonging advantage compared resveratrol treating ALD. This suggests holds promise as potential agent ameliorates cellular damage likely by binding activating suppressing activity.

Language: Английский

Citations

0