Recent Advances and Challenges in the Production of Hydroxylated Natural Products Using Microorganisms DOI Creative Commons
Chang Sun,

Rumei Zeng,

Tianpeng Chen

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 604 - 604

Published: Nov. 26, 2024

Hydroxylation reaction is a significant source of structural diversity in natural products (NPs), playing crucial role improving the bioactivity, solubility, and stability product molecules. This review summarizes latest research progress field hydroxylation, focusing on several key hydroxylases involved biosynthesis NPs, including cytochrome P450 monooxygenases, α-ketoglutarate-dependent hydroxylases, flavin-dependent monooxygenases. These enzymes achieve selective hydroxylation modification various such as terpenoids, flavonoids, steroids, through different catalytic mechanisms. systematically recent advances amino acids, lipids, phenylpropanoids, demonstrating potential synthetic biology strategies constructing artificial biosynthetic pathways producing hydroxylated derivatives. Through metabolic engineering, enzyme genetic combined with intelligence-assisted technologies, series engineered strains have been successfully constructed for efficient production NPs their derivatives, achieving synthesis NPs. has provided new avenues drug development, functional food, biomaterial also offered ideas industrial these compounds. In future, integrating pathway design, directed evolution, dynamic regulation, intelligence technology expected to further expand application enzyme-catalyzed reactions green complex promoting heights.

Language: Английский

Tea Catechins in Green Tea Inhibit the Activity of SARS-CoV-2 Main Protease via Covalent Adduction DOI
Yoji Kato,

Sakiko Suzuki,

Akari Higashiyama

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

We herein examined the inhibitory effects of tea catechins on SARS-CoV-2 main protease (Mpro). Among analyzed, epigallocatechin 3-(3″-O-methyl)gallate, gallate (EGCG), gallocatechin, gallocatechin gallate, and inhibited recombinant Mpro in a dose-dependent manner. Peptide mapping revealed that preferentially formed covalent bonds with five sequences strongest activity at C145 active site. Fragmentation analysis indicated 184 cleavages from peptides containing C145, corresponding to D ring, suggesting B ring was attached C145. When 10 bottled teas were incubated Mpro, four green enzyme by over 80%, whereas blended barley showed no effect. EGCG reacted covalently within cells when cultured expressing Mpro. This is first study report direct binding between cells. suggests can inhibit infected

Language: Английский

Citations

0
Recent Advances and Challenges in the Production of Hydroxylated Natural Products Using Microorganisms DOI Creative Commons
Chang Sun,

Rumei Zeng,

Tianpeng Chen

et al.

Fermentation, Journal Year: 2024, Volume and Issue: 10(12), P. 604 - 604

Published: Nov. 26, 2024

Hydroxylation reaction is a significant source of structural diversity in natural products (NPs), playing crucial role improving the bioactivity, solubility, and stability product molecules. This review summarizes latest research progress field hydroxylation, focusing on several key hydroxylases involved biosynthesis NPs, including cytochrome P450 monooxygenases, α-ketoglutarate-dependent hydroxylases, flavin-dependent monooxygenases. These enzymes achieve selective hydroxylation modification various such as terpenoids, flavonoids, steroids, through different catalytic mechanisms. systematically recent advances amino acids, lipids, phenylpropanoids, demonstrating potential synthetic biology strategies constructing artificial biosynthetic pathways producing hydroxylated derivatives. Through metabolic engineering, enzyme genetic combined with intelligence-assisted technologies, series engineered strains have been successfully constructed for efficient production NPs their derivatives, achieving synthesis NPs. has provided new avenues drug development, functional food, biomaterial also offered ideas industrial these compounds. In future, integrating pathway design, directed evolution, dynamic regulation, intelligence technology expected to further expand application enzyme-catalyzed reactions green complex promoting heights.

Language: Английский

Citations

1