Hydrogen gas therapy: A promising approach for sepsis management post-burn injury by modulating inflammation, oxidative stress, and wound healing DOI Open Access
Yu Pan, Nan Hong,

Genwang Wang

et al.

CytoJournal, Journal Year: 2025, Volume and Issue: 22, P. 46 - 46

Published: April 25, 2025

Objective Burns refers to a severe form of trauma that often leads localized and systemic inflammatory responses, oxidative stress, immune dysfunction. Patients with burns are highly susceptible the development postburn sepsis, condition influenced by multiple factors, such as bacterial infection burn wound, alterations in status, excessive release mediators. This study aimed investigate mechanisms which hydrogen gas treatment exerts its effects on focus influence wound healing. Material Methods work employed vitro assays Sprague–Dawley (SD) rat skin fibroblasts (RSFs) assess serum cell proliferation through methylthiazolyldiphenyltetrazolium bromide apoptosis flow cytometry Annexin V-fluorescein isothiocyanate/propidium iodide staining. In addition, an enzyme-linked immunosorbent assay was performed quantify cytokines stress markers treated serum. Western blotting (WB) analysis conducted signaling pathway modulation. The sepsis models SD rats were segregated into three experimental groups: healthy normal control group, + (2%) group. Wound healing monitored, contraction rates recorded histological assessments using hematoxylin eosin Masson’s trichrome staining evaluate tissue repair collagen deposition. Results showed reduced fibroblast increased ( P < 0.01), mitigated rescuing viability reducing 0.01). Enzyme-linked revealed serum-induced increases levels markers, decreases antioxidant enzymes reversed 0.05). WB suggested gas’s anti-inflammatory proliferative modulating pathways vivo , considerably improved healing, accelerated enhanced Plasma analyses indicated local from gas. Conclusion Hydrogen demonstrates potential therapeutic efficacy management promoting These findings provide scientific evidence supporting adjunctive strategy for sepsis.

Language: Английский

Bifidobacterium longum subspecies infantis CCFM111 mitigates allergic rhinitis in mice through adjusting intestinal microecology and elevating indole-3-carboxaldehyde levels DOI
Yu Wang, Zhangming Pei, Xiaomeng Li

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106188 - 106188

Published: Feb. 1, 2025

Language: Английский

Citations

0
Exacerbation of Asthma Airway Inflammation by HOCl Exposure: Insights from Fecal Microbiota and Metabolomics DOI
Yingjun Chen, S. Stephen Yi,

Qian Wang

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 490, P. 137796 - 137796

Published: Feb. 28, 2025

Language: Английский

Citations

0
The tryptophan metabolite 3-hydroxyanthranilic acid alleviates hyperoxia-induced bronchopulmonary dysplasia via inhibiting ferroptosis DOI Creative Commons

Qiqi Ruan,

Y. Peng, X. Yi

et al.

Redox Biology, Journal Year: 2025, Volume and Issue: 82, P. 103579 - 103579

Published: March 9, 2025

Bronchopulmonary dysplasia (BPD) is a prevalent chronic respiratory condition in preterm infants with an increasing incidence, severely affecting their survival rate and quality of life. Exploring the underlying mechanisms BPD helps to develop novel effective therapeutic strategies. In this study, integrated metabolomic analyses tracheal aspirates (TAs) from non-BPD infants, along lung tissues hyperoxia-induced experimental neonatal rats control rats, demonstrated that was associated significant reduction 3-hydroxyanthranilic acid (3-HAA), which confirmed be partly caused by tryptophan-metabolizing enzyme disorders. vivo vitro models were subsequently established assess efficacy 3-HAA relation BPD. Compared group, nebulization improved development suppressed inflammation rats. Limited proteolysis-small molecule mapping (LiP-SMap) proteomic analysis revealed involvement ferroptosis pathway mechanism alleviated injury. Ferroptosis identified detecting Fe2+ levels, malondialdehyde (MDA), 4-HNE, total aldehydes, mitochondrial morphology, ferroptosis-associated protein mRNA expression, dysregulation indeed ameliorated vivo. Furthermore, combination LiP-SMap, molecular docking, SPR Co-IP can bind directly FTH1 disrupt nuclear receptor coactivator 4 (NCOA4)-FTH1 interaction. conclusion, our study first reveal linked 3-HAA, could inhibit targeting FTH1, thereby alleviating injury alveolar type II epithelial cells, highlighting potential for clinical applications

Language: Английский

Citations

0
The Effect of Hydrogen-Rich Water on Retinal Degeneration in the Outer Nuclear Layer of Simulated Weightlessness Rats DOI

Yuxue Mu,

Wenhao Li,

Dongyu Wei

et al.

Life Sciences in Space Research, Journal Year: 2025, Volume and Issue: 45, P. 158 - 169

Published: March 16, 2025

Language: Английский

Citations

0
Advances in gut-lung axis research: clinical perspectives on pneumonia prevention and treatment DOI Creative Commons
Fang Ye, Linnan Li, Jiao Wang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 22, 2025

In recent years, the study of interaction between gut microbiota and distant organs such as heart, lungs, brain, liver has become a hot topic in field microbiology. With deeper understanding its immune regulation mechanisms action, these findings have increasingly highlighted their guiding value clinical practice. The is not only largest digestive organ human body but also habitat for most microorganisms. Imbalances microbial communities been associated with various lung diseases, allergic asthma cystic fibrosis. Furthermore, significant impacts on metabolic function responses. Their metabolites regulate gastrointestinal systems may affect lungs brain. As one common types respiratory system diseases worldwide, pulmonary infections high morbidity mortality rates. Pulmonary caused by dysfunction can lead to problems like diarrhea, further resulting imbalances within complex interactions that are abnormal manifestations under disequilibrium conditions. Meanwhile, interventions significantly modulate composition microbiota, alteration subsequently indicate susceptibility even contribute prevention or progression. This review delves into infections, elucidating latest advancements gut-lung axis research providing fresh perspective treatment pneumonia.

Language: Английский

Citations

0
Hydrogen gas therapy: A promising approach for sepsis management post-burn injury by modulating inflammation, oxidative stress, and wound healing DOI Open Access
Yu Pan, Nan Hong,

Genwang Wang

et al.

CytoJournal, Journal Year: 2025, Volume and Issue: 22, P. 46 - 46

Published: April 25, 2025

Objective Burns refers to a severe form of trauma that often leads localized and systemic inflammatory responses, oxidative stress, immune dysfunction. Patients with burns are highly susceptible the development postburn sepsis, condition influenced by multiple factors, such as bacterial infection burn wound, alterations in status, excessive release mediators. This study aimed investigate mechanisms which hydrogen gas treatment exerts its effects on focus influence wound healing. Material Methods work employed vitro assays Sprague–Dawley (SD) rat skin fibroblasts (RSFs) assess serum cell proliferation through methylthiazolyldiphenyltetrazolium bromide apoptosis flow cytometry Annexin V-fluorescein isothiocyanate/propidium iodide staining. In addition, an enzyme-linked immunosorbent assay was performed quantify cytokines stress markers treated serum. Western blotting (WB) analysis conducted signaling pathway modulation. The sepsis models SD rats were segregated into three experimental groups: healthy normal control group, + (2%) group. Wound healing monitored, contraction rates recorded histological assessments using hematoxylin eosin Masson’s trichrome staining evaluate tissue repair collagen deposition. Results showed reduced fibroblast increased ( P < 0.01), mitigated rescuing viability reducing 0.01). Enzyme-linked revealed serum-induced increases levels markers, decreases antioxidant enzymes reversed 0.05). WB suggested gas’s anti-inflammatory proliferative modulating pathways vivo , considerably improved healing, accelerated enhanced Plasma analyses indicated local from gas. Conclusion Hydrogen demonstrates potential therapeutic efficacy management promoting These findings provide scientific evidence supporting adjunctive strategy for sepsis.

Language: Английский

Citations

0