Edgeworthia gardneri (Wall.) Meisn Mitigates CCL4-induced liver injury in mice by modulating gut microbiota, boosting antioxidant defense, and reducing inflammation

Ecotoxicology and Environmental Safety, Journal Year: 2025, Volume and Issue: 293, P. 118042 - 118042

Published: March 1, 2025

Herbal medicine has become an area of growing global scientific interest. The prime objective this study was to investigate the protective role Edgeworthia gardneri (Wall.) Meisn (EGM polysaccharide) against carbon tetrachloride (CCl4)-induced liver injury in mice. Forty-five ICR mice were randomly divided into three groups (n = 15): IC, IM, and IT. IT group received EGM polysaccharide solution (50 mg/kg) daily, while IC IM administered equivalent volume normal saline. intraperitoneally injected with a mixture CCl4 olive oil at 1:1 (v/v) (2 mL/kg) every 3 days. Our results showed that significantly (p < 0.05) reduced pathological hepatic alterations increased index caused by CCl4. Moreover, therapy 0.001) levels antioxidant enzymes, such as glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), total capacity (T-AOC) malondialdehyde (MDA) content dose-dependent manner. Notably, alleviated inflammatory cascades evidenced decreased serum interleukin-6 (IL-6), interleukin-1β (IL-1β), tumor necrosis factor- α (TNF-α) under administration. Furthermore, 16 s rRNA gene sequencing exhibited abundance probiotics bacteria, Unclassified_Lachnospiraceae, pathogenic bacterial texas like Brevundimonas Candidatus_Nitrocosmicu. Conclusively, protects CCl4-induced oxidative stress inflammation alleviates through beneficial gut microbiota modulations. current suggests is effective agent counteracting damage.

Language: Английский

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Caffeic acid activates Nrf2 enzymes, providing protection against oxidative damage induced by ionizing radiation

Brain Research Bulletin, Journal Year: 2025, Volume and Issue: unknown, P. 111325 - 111325

Published: March 1, 2025

Caffeic acid (CA) is a prevalent polyphenolic compound commonly found in various plant-derived foods. Due to its diverse pharmacological properties, including antioxidant activity, cardiovascular protection, and immune regulation, CA has garnered significant attention. Ionizing radiation (IR) extensively utilized across industrial sectors, agriculture, defense applications, scientific research, clinical medicine; however, the detrimental effects of on human health cannot be ignored. IR can directly damage DNA, proteins, lipids within macromolecules or ionize water molecules generate substantial quantities free radicals that indirectly harm cells, especially those brain which are highly susceptible exposure. Consequently, effective strategies for preventing treating IR-induced neurological represent an urgent medical challenge necessitates resolution. Our study aims investigate protective against neuronal cell along with elucidating potential mechanisms action. The results indicate covalently modify active cysteine residues Keap1 protein altering conformation; this modification disrupts interaction between Nrf2 while facilitating Nrf2's translocation into nucleus where it activates downstream expression cellular factors such as heme oxygenase-1 (HO-1), NAD(P)H: quinone oxidoreductase 1 (NQO1), Thioredoxin Reductase-1 (TrxR1) other play role countering radiation-induced damage. In conclusion, emerges radioprotective agent capable exerting antiradiation effects. findings provide valuable insights developing novel therapeutic agents aimed at impairment.

Language: Английский

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Effect of basic fibroblast growth factor on hypoxia-inducible factor (HIF)-1α expression (Exp) and HIF-1 transcription in breast Cancer cell through PI-3 K Akt signaling

Cytokine, Journal Year: 2025, Volume and Issue: 187, P. 156859 - 156859

Published: Jan. 17, 2025

Language: Английский

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Pandanus Amaryllifolius Roxb. Polyphenol Extract Alleviates NAFLD via Regulating Gut Microbiota and AMPK/AKT/mTOR Signaling Pathway

Foods, Journal Year: 2025, Volume and Issue: 14(6), P. 1000 - 1000

Published: March 15, 2025

With the drastic changes in lifestyle, nonalcoholic fatty liver disease (NAFLD) has become a widespread health problem. Natural actives such as polyphenols have multi-target, multi-mechanism characteristics, and offer new opportunities for NAFLD treatment. This study established high-fat diet (HFD)-induced model mice to investigate molecular mechanism of Pandanus amaryllifolius Roxb. polyphenol extract (PAE) alleviating NAFLD. The results showed that PAE significantly inhibited HFD-induced obesity, maintained glucose homeostasis, mitigated oxidative damage tissue, reduced steatosis. Moreover, treatment remarkably reversed 16 endogenous DMs, affected glycerophospholipid metabolism, which increased levels phosphatidylcholine phosphatidylethanolamine, down-regulated choline sn-glyceropl-3P. Further validation revealed was able prevent progression by regulating AMPK/AKT/mTOR signaling pathway enhance autophagy levels. Meanwhile, restored balance gut microbiota mainly increasing relative abundance Bacteroidetes, decreasing Firmicutes ratio Firmicutes/Bacteroidetes. Overall, findings highlight alleviates may be related regulation microbes pathway, enriching health-promoting effects on

Language: Английский

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Multi-omics joint analysis: Pachymic acid ameliorated non-alcoholic fatty liver disease by regulating gut microbiota

Food Research International, Journal Year: 2025, Volume and Issue: unknown, P. 116178 - 116178

Published: March 1, 2025

Language: Английский

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Totum-448, a polyphenol-rich plant extract, decreases hepatic steatosis and inflammation in diet-induced MASLD mice

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

The increasing prevalence of obesity-driven metabolic dysfunction-associated steatotic liver disease (MASLD) urges the development new therapeutic strategies. Totum-448 is a unique patented combination polyphenol-rich plant extracts designed to reduce hepatic steatosis, risk factor for steatohepatitis and type 2 diabetes. In this study, we investigated effects on homeostasis in diet-induced MASLD mice. For purpose, male C57Bl6/J mice were fed high-fat diet with sucrose-containing drinking water 12 weeks, followed by supplementation or without 4 weeks. Body weight/composition, caloric intake, plasma parameters whole-body glucose tolerance measured throughout study fecal microbiota composition was determined 16S sequencing. Hepatic transcriptomic/lipidomic profiles immune cell assessed histological/biochemical assays, RNA sequencing, MS-based lipidomics, spectral flow cytometry. We found that significantly lowered hyperinsulinemia systemic intolerance affecting body weight, fat mass, calorie feces production composition. Furthermore, decrease activity score macrovesicular triglycerides cholesterol contents, alanine aminotransferase levels observed. also reduced inflammatory pro-fibrotic transcriptomic signatures decreased both MASLD-induced loss tissue-resident Kupffer cells recruitment monocyte-derived pro-inflammatory macrophages. Altogether, reduces steatosis inflammation insulin-resistant, steatotic, obese mice, dual effect likely contributes improved homeostasis. Supplementation may therefore constitute promising novel nutritional approach patients.

Language: Английский

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A strategy for liver selective NRF2 induction via cytochrome P450 activated prodrugs with low activity in hypoxia

Journal of Biological Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 108487 - 108487

Published: April 1, 2025

Activation of the transcription factor NRF2 has been shown to be a promising therapeutic approach in treatment hepatosteatosis. is believed exert beneficial effects by upregulating cellular oxidative defense mechanisms and inhibiting inflammation. However, major concern associated with long-term activators are drug side effects, including promotion tumorigenesis. Many function forming cysteine adducts KEAP1, which normally mediates ubiquitination degradation NRF2. In this study we identified activator prodrugs 4-methylcatechol tert-butylhydroquinone. These converted into their active metabolites liver selective, cytochrome P450 dependent manner resulting activation. Unexpectedly, also found that number activating compounds, tert-butylhydroquinone, show markedly lower activity under hypoxic conditions compared normoxia. Our findings suggest these inducers consequence less potent adduct formation KEAP1. The inducing compounds hypoxia may limit tumor promoting induction. provides an important proof concept it possible selectively activate for hepatosteatosis while avoiding tumorigenic as well activation other tissues.

Language: Английский

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Rhizoma Atractylodis Macrocephalae reduces HFD-induced NAFLD in mice through activated AMPK mediated inhibition of fatty acid synthesis

Liver Research, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Extracts from Tartary Buckwheat Sprouts Restricts Oxidative Injury Induced by Hydrogen Peroxide in HepG2 by Upregulating the Redox System

Foods, Journal Year: 2024, Volume and Issue: 13(23), P. 3726 - 3726

Published: Nov. 21, 2024

Oxidative stress, which results from an overproduction of reactive oxygen species (ROS), can cause damage that may contribute to a range metabolic disorders. Antioxidants are considered upregulate the activity antioxidant enzymes, crucial for eliminating excess ROS and safeguarding body against oxidative stress-induced damage. In present study, effect polyphenol extracts tartary buckwheat sprouts (TBSE) on redox system HepG2-cell-induced injury by hydrogen peroxide were investigated evaluating protective mechanism (TBS). The revealed TBSE had sprouted period 10 days possessed six predominant phenolic compounds, ranked most abundant least: chlorogenic acid, syringic caffeic rutin, ferulic quercetin. could successfully inhibit H

Language: Английский

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