Identification of Stable Reference miRNAs for miRNA Expression Analysis in Adult Neurogenesis Across Mouse and Human Tissues DOI Creative Commons

Daniella Liana Levitis,

Jiawen Si, K. V. Ravishankar

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2060 - 2060

Published: Dec. 13, 2024

Accurate normalization in miRNA studies requires the use of appropriate endogenous controls, which can vary significantly depending on cell types, treatments, and physiological or pathological conditions. This study aimed to identify suitable controls for neural progenitor cells (NPCs) hippocampal tissues, both play crucial roles neurogenesis. Using small RNA sequencing, we identified most stable miRNAs primary mouse NPCs tissues accessed their stability using NormFinder analysis. Six miRNAs—miR-181d-5p, miR-93-5p, miR-103-3p, let-7d-5p, miR-26a-5p, miR-125a-5p—demonstrated high were evaluated suitability as across multiple experimental All selected exhibited consistent expression NE-4C line but not ReNcells, a human line. For only miR-186-5p, one known reference tested comparison, showed expression. Notably, miR-103-3p let-7d-5p stably expressed from samples absent brain pericytes, microvascular endothelial cells, SVG p12 fetal glial is first optimal adult neurogenesis samples, providing reliable options improving accuracy reproducibility analyses research.

Language: Английский

Intervention Role of APOE in CNS Diseases: APOE Actions and APOE Neurogenesis Capability DOI

Wenhua Li,

Suya Ma, Min Li

et al.

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 22, 2025

Language: Английский

Citations

0
Identification of Stable Reference miRNAs for miRNA Expression Analysis in Adult Neurogenesis Across Mouse and Human Tissues DOI Creative Commons

Daniella Liana Levitis,

Jiawen Si, K. V. Ravishankar

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(24), P. 2060 - 2060

Published: Dec. 13, 2024

Accurate normalization in miRNA studies requires the use of appropriate endogenous controls, which can vary significantly depending on cell types, treatments, and physiological or pathological conditions. This study aimed to identify suitable controls for neural progenitor cells (NPCs) hippocampal tissues, both play crucial roles neurogenesis. Using small RNA sequencing, we identified most stable miRNAs primary mouse NPCs tissues accessed their stability using NormFinder analysis. Six miRNAs—miR-181d-5p, miR-93-5p, miR-103-3p, let-7d-5p, miR-26a-5p, miR-125a-5p—demonstrated high were evaluated suitability as across multiple experimental All selected exhibited consistent expression NE-4C line but not ReNcells, a human line. For only miR-186-5p, one known reference tested comparison, showed expression. Notably, miR-103-3p let-7d-5p stably expressed from samples absent brain pericytes, microvascular endothelial cells, SVG p12 fetal glial is first optimal adult neurogenesis samples, providing reliable options improving accuracy reproducibility analyses research.

Language: Английский

Citations

0