An Electron Paramagnetic Resonance Study of the Superoxide-Scavenging and Redox-Modulating Effects of Lecithinized Superoxide Dismutase in the Bloodstream DOI Creative Commons
Dessislava Lazarova,

Plamen Getsov,

Rumiana Bakalova

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(9), P. 1882 - 1882

Published: April 23, 2025

Lecithinized superoxide dismutase (PC-SOD) was found to have a significantly improved half-life in the bloodstream and better pharmacological effects compared with unmodified SOD. However, there is no direct evidence that parenterally administered PC-SOD decreases levels blood tissues vivo. In present study, we investigated ability of versus SOD as scavenger mice subjected oxidative stress. Experiments were performed on lipopolysaccharide (LPS) mouse model acute inflammation known be accompanied by overproduction blood. The divided into four groups: untreated (healthy; n = 6), LPS-treated (n 7), LPS/SOD-treated LPS/PC-SOD-treated 7) mice. injected intravenously. Blood samples collected at time intervals analyzed electron paramagnetic resonance (EPR) spectroscopy using nitroxide probe, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (CMP). following observed: (i) healthy mice, EPR signal lower control (p < 0.001) 0.01); (ii) identical control; (iii) immediately after enzyme injection, 0.01) 0.05). effect disappeared 30 min 1 h injection. (iv) 0.05), even within data indicate characterized high reducing capacity, while capacity. decreased production short-lived min. effectively restored redox balance level two hours more pronounced long-lasting those possible reason longer bloodstream, its stability, slower clearance from circulation due increased hydrophobicity interaction plasma proteins. are discussed context recent clinical trials showing promising pharmaceutical product for adjuvant therapy variety pathologies inflammation, imbalance,

Language: Английский

An Electron Paramagnetic Resonance Study of the Superoxide-Scavenging and Redox-Modulating Effects of Lecithinized Superoxide Dismutase in the Bloodstream DOI Creative Commons
Dessislava Lazarova,

Plamen Getsov,

Rumiana Bakalova

et al.

Molecules, Journal Year: 2025, Volume and Issue: 30(9), P. 1882 - 1882

Published: April 23, 2025

Lecithinized superoxide dismutase (PC-SOD) was found to have a significantly improved half-life in the bloodstream and better pharmacological effects compared with unmodified SOD. However, there is no direct evidence that parenterally administered PC-SOD decreases levels blood tissues vivo. In present study, we investigated ability of versus SOD as scavenger mice subjected oxidative stress. Experiments were performed on lipopolysaccharide (LPS) mouse model acute inflammation known be accompanied by overproduction blood. The divided into four groups: untreated (healthy; n = 6), LPS-treated (n 7), LPS/SOD-treated LPS/PC-SOD-treated 7) mice. injected intravenously. Blood samples collected at time intervals analyzed electron paramagnetic resonance (EPR) spectroscopy using nitroxide probe, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (CMP). following observed: (i) healthy mice, EPR signal lower control (p < 0.001) 0.01); (ii) identical control; (iii) immediately after enzyme injection, 0.01) 0.05). effect disappeared 30 min 1 h injection. (iv) 0.05), even within data indicate characterized high reducing capacity, while capacity. decreased production short-lived min. effectively restored redox balance level two hours more pronounced long-lasting those possible reason longer bloodstream, its stability, slower clearance from circulation due increased hydrophobicity interaction plasma proteins. are discussed context recent clinical trials showing promising pharmaceutical product for adjuvant therapy variety pathologies inflammation, imbalance,

Language: Английский

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