Diversity and functional specialization of oyster immune cells uncovered by integrative single cell level investigations DOI Open Access

Sébastien de La Forest Divonne,

Juliette Pouzadoux,

Océane Romatif

et al.

Published: Nov. 13, 2024

Mollusks are a major component of animal biodiversity and play critical role in ecosystems global food security. The Pacific oyster, Crassostrea (Magallana) gigas , is the most farmed bivalve mollusk world becoming model species for invertebrate biology. Despite extensive research on hemocytes, immune cells bivalves, their characterization remains elusive. Here we were able to extensively characterize diverse hemocytes identified at least seven functionally distinct cell types three hematopoietic lineages. A combination single-cell RNA sequencing, quantitative cytology, sorting, functional assays pseudo-time analyses was used deliver comprehensive view hemocyte types. This integrative analysis enabled us reconcile molecular cellular data identify performing specialized functions, such as phagocytosis, reactive oxygen production, copper accumulation, expression antimicrobial peptides. study emphasized need more depth studies immunity mollusks non-model invertebrates set ground further comparative immunology level.

Language: Английский

Bivalve Haemocyte Subpopulations: A Review DOI Creative Commons

Nuria R. de la Ballina,

Francesco Maresca, Asunción Cao

et al.

Frontiers in Immunology, Journal Year: 2022, Volume and Issue: 13

Published: April 8, 2022

Bivalve molluscs stand out for their ecological success and key role in the functioning of aquatic ecosystems, while also constituting a very valuable commercial resource. Both production bivalves depend on effective immune defence function, which haemocytes play central acting as both undertaker cellular immunity supplier humoral immunity. Bivalves have different types haemocytes, perform functions. Hence, identification cell subpopulations functional characterisation responses is essential to fully understand system bivalves. Nowadays, there not unified nomenclature that applies all Characterisation bivalve haemocyte often combined with 1) other multiple parameter assays determine differences between immune-related physiological activities, such phagocytosis, oxidative stress apoptosis; 2) response stressors pathogens, temperature, acidification pollution. This review summarises major most recent findings classification main molluscs.

Language: Английский

Citations

90
Hemocytes of bivalve mollusks as cellular models in toxicological studies of metals and metal-based nanomaterials DOI
Nanyan Weng, Jie Meng, Shouliang Huo

et al.

Environmental Pollution, Journal Year: 2022, Volume and Issue: 312, P. 120082 - 120082

Published: Aug. 31, 2022

Language: Английский

Citations

34
Diversity and functional specialization of oyster immune cells uncovered by integrative single cell level investigations DOI Open Access

Sébastien de La Forest Divonne,

Juliette Pouzadoux,

Océane Romatif

et al.

Published: March 25, 2025

Mollusks are a major component of animal biodiversity and play critical role in ecosystems global food security. The Pacific oyster, Crassostrea (Magallana) gigas , is the most farmed bivalve mollusk world becoming model species for invertebrate biology. Despite extensive research on hemocytes, immune cells bivalves, their characterization remains elusive. Here we were able to extensively characterize diverse hemocytes identified at least seven functionally distinct cell types three hematopoietic lineages. A combination single-cell RNA sequencing, quantitative cytology, sorting, functional assays pseudo-time analyses was used deliver comprehensive view hemocyte types. This integrative analysis enabled us reconcile molecular cellular data identify performing specialized functions, such as phagocytosis, reactive oxygen production, copper accumulation, expression antimicrobial peptides. study emphasized need more depth studies immunity mollusks non-model invertebrates set ground further comparative immunology level.

Language: Английский

Citations

0
Diversity and functional specialization of oyster immune cells uncovered by integrative single cell level investigations DOI Open Access

Sébastien de La Forest Divonne,

Juliette Pouzadoux,

Océane Romatif

et al.

Published: April 11, 2025

Mollusks are a major component of animal biodiversity and play critical role in ecosystems global food security. The Pacific oyster, Crassostrea (Magallana) gigas , is the most farmed bivalve mollusk world becoming model species for invertebrate biology. Despite extensive research on hemocytes, immune cells bivalves, their characterization remains elusive. Here we were able to extensively characterize diverse hemocytes identified at least seven functionally distinct cell types three hematopoietic lineages. A combination single-cell RNA sequencing, quantitative cytology, sorting, functional assays pseudo-time analyses was used deliver comprehensive view hemocyte types. This integrative analysis enabled us reconcile molecular cellular data identify performing specialized functions, such as phagocytosis, reactive oxygen production, copper accumulation, expression antimicrobial peptides. study emphasized need more depth studies immunity mollusks non-model invertebrates set ground further comparative immunology level.

Language: Английский

Citations

0
Immune diversity in lophotrochozoans, with a focus on recognition and effector systems DOI Creative Commons
Yongnan Li,

Yu Xue,

Zhangjie Peng

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2023, Volume and Issue: 21, P. 2262 - 2275

Published: Jan. 1, 2023

Lophotrochozoa is one of the most species-rich but immunologically poorly explored phyla. Although lack acquired response in a narrow sense, lophotrochozoans possess various genetic mechanisms that enhance diversity and specificity innate immune system. Here, we review recent advances comparative immunology studies with focus on recognition effector systems. Haemocytes coelomocytes are general important yet understudied player. Comparative genomics suggest expansion functional divergence lophotrochozoan reorganization systems not as "homogeneous simple" thought including large-scale molecular pattern receptors (PRRs) (TLRs, RLRs, lectins, etc.) signaling adapters (MyD88s etc.), significant domain recombination (RLR, NLRs, extensive somatic fibrinogenrelated proteins (FREPs) snails. Furthermore, there repeatedly identified generate diversity, high polymorphism antimicrobial peptides (AMPs), reactive oxygen nitrogen species (RONS) cytokines. Finally, argue next generation omics tools recently emerged genome editing technicism will revolutionize our understanding system perspective.

Language: Английский

Citations

7
Chromosome-level genome assembly of the European flat oyster (Ostrea edulis) provides insights into its evolution and adaptation DOI
Xinchun Li, Yitian Bai, Zhen Dong

et al.

Comparative Biochemistry and Physiology Part D Genomics and Proteomics, Journal Year: 2022, Volume and Issue: 45, P. 101045 - 101045

Published: Nov. 29, 2022

Language: Английский

Citations

9
A common garden experiment supports a genetic component underlying the increased resilience of common cockle (Cerastoderma edule) to the parasite Marteilia cochillia DOI Creative Commons
António Villalba,

Raquel M. Coimbra,

Marina Pampín

et al.

Evolutionary Applications, Journal Year: 2023, Volume and Issue: 16(11), P. 1789 - 1804

Published: Oct. 17, 2023

Abstract The common cockle is a valuable bivalve species inhabiting the Atlantic European coasts. parasite Marteilia cochillia has devastated beds in southern Galician (NW Spain) rias since 2012. Previous data suggested that cockles from Ría de Arousa acquired some resilience to this through natural selection after consecutive annual marteiliosis outbreaks and candidate markers associated with were identified using population genomics transcriptomics approaches. Here, garden experiment was performed naïve stock (from Muros‐Noia) an affected Arousa) test hypothesis. Breeders both stocks used produce seed cohorts at hatchery, which pre‐grown raft (outdoor nursery stage) deployed two shellfish by (growing‐out stage). In beds, showed high prevalence fully depleted short period, while barely evidence of marteiliosis. A set 45 SNPs putatively fitted for MassARRAY genotyping check their role differential detected between stocks. Though no significant differentiation found neutral markers, 28 divergence them, suggesting these involved directional during eight generations (to most) pressure (long‐term selection). Furthermore, signals also along outbreak growing‐out stage (short‐term selection) six SNPs, all shared long‐term evaluation, consistent according infection severity. Some located within immune genes pertaining families such as proteasome, ubiquitin, tumor necrosis factor, glutathione S‐transferase. These resilience‐associated will be useful recover production Galicia.

Language: Английский

Citations

2
Examination of the role of hypoxia-inducible factor-1α (HIF-1α) in preventing hemocyte apoptosis in whiteleg shrimp (Litopenaeus vannamei) DOI
Zhihai Huang, Weiliang Guan,

Xiaobo Wei

et al.

Aquaculture, Journal Year: 2022, Volume and Issue: 563, P. 738905 - 738905

Published: Oct. 7, 2022

Language: Английский

Citations

3
Exploring the Internal Defense System of Cerastoderma glaucum (Bruguière, 1789) Exposed to Pristine Microplastics: The Sentinel Role of Haemocytes as Biomarkers DOI Creative Commons
Alessio Alesci,

Davide Di Paola,

Sebastián Marino

et al.

Fishes, Journal Year: 2024, Volume and Issue: 9(6), P. 241 - 241

Published: June 20, 2024

The ubiquitous presence of microplastics (MPs) in the environment is a major concern for conservation biodiversity. Numerous studies show detrimental effects MPs on marine species, especially because their small size and capacity to absorb organic contaminants from surroundings. bivalve mollusk Cerastoderma glaucum (Bruguière, 1789), its wide geographic distribution immobile feeding habits, can be used as sentinel bioindicator species. By examining presence, localization, co-localization Toll-like receptor 2 (TLR2) inducible nitric oxide synthetase (iNOS), this study aims evaluate response internal defense system C. pristine through employment confocal microscopy bioinformatics techniques. results haemocytes immunoreactive antibodies tested; particular, higher number TLR2-positive observed group exposed MPs. These findings suggest that play key biomarker role sentinels environmental pollutants. Furthermore, analyses tested confirmed an evolutionary these molecules. data highlight critical phagocytosis identifying ecosystem damage are helpful developing biosensors with less negative environments which they applied.

Language: Английский

Citations

0
Diversity and functional specialization of oyster immune cells uncovered by integrative single cell level investigations DOI Creative Commons

Sébastien de La Forest Divonne,

Juliette Pouzadoux,

Océane Romatif

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 23, 2024

Abstract Mollusks are a major component of animal biodiversity and play critical role in ecosystems global food security. The Pacific oyster, Crassostrea (Magallana) gigas , is the most farmed bivalve mollusk world becoming model species for invertebrate biology. Despite extensive research on hemocytes, immune cells bivalves, their characterization remains elusive. Here we were able to extensively characterize diverse hemocytes identified at least seven functionally distinct cell types three hematopoietic lineages. A combination single-cell RNA sequencing, quantitative cytology, sorting, functional assays pseudo-time analyses was used deliver comprehensive view hemocyte types. This integrative analysis enabled us reconcile molecular cellular data identify performing specialized functions, such as phagocytosis, reactive oxygen production, copper accumulation, expression antimicrobial peptides. study emphasized need more depth studies immunity mollusks non-model invertebrates set ground further comparative immunology level.

Language: Английский

Citations

0