Ecotoxicology and Environmental Safety,
Journal Year:
2022,
Volume and Issue:
236, P. 113471 - 113471
Published: April 2, 2022
Ammonia
gas,
a
toxic
environmental
pollutant,
is
vital
component
of
PM2.5
aerosols,
and
can
decrease
human
animal
immunity.
Peripheral
blood
lymphocytes
(PBLs)
are
main
immune
cells.
Nevertheless,
poisoning
mechanism
PBLs
under
ammonia
exposure
remains
unclear.
Here,
we
established
an
model
chicken
to
explore
ammonia-caused
apoptosis
in
PBLs.
Cell
viability
rate
were
detected
using
CCK8
assay
flow
cytometry,
respectively.
Mitochondrial
membrane
potential
(MMP)
was
observed
fluorescent
staining.
In
addition,
qRT-PCR
performed
measure
mRNA
levels
apoptosis-related
genes
(tumor
necrosis
factor-α
(TNF-α),
tumor
factor
receptor
1
(TNFR1),
TNF
receptor-associated
death
domain
(TRADD),
Fas-associated
(FADD),
Caspase-8,
BH3-interacting
agonist
(Bid),
Bcl-2-associated
X
protein
(Bax),
Bcl-2
homologous
antagonist/killer
(Bak),
B-cell
lymphoma-2
(Bcl-2),
Cytochrome-c
(Cytc),
apoptotic
protease
activating
factor-1
(APAF1),
Caspase-9,
Caspase-3),
immune-related
(interferon-γ
(IFN-γ),
interleukin-2
(IL-2),
IL-4,
IL-6,
IL-1β,
IL-10,
transforming
growth
factor-β1
(TGF-β1),
IL-17,
IL-21,
IL-22),
heat
shock
(HSP)
(HSP25,
HSP40,
HSP60,
HSP70,
HSP90,
HSP110),
as
well
miR-27b-3p.
Western
blot
used
determine
factors
(TNF-α,
Bcl-2,
HSPs
(HSP40,
HSP90).
The
results
indicated
that
TRADD,
FADD,
APAF1
target
miR-27b-3p,
miR-27b-3p
participated
molecular
through
targeting
TNF-α/TNFR1/Caspase-8
pathway-triggered
Bid/Cytc/Caspase-9
mitochondrial
pathway
ammonia-treated
our
findings
demonstrated
excess
led
immunosuppression
via
Th1/Th2
imbalance
Treg/Th17
imbalance.
Simultaneously,
stress
activated
HSPs.
summary,
for
the
first
time,
data
HSPs-triggered
exposure.
Our
provided
new
insight
into
important
reference
risk
assessment
related
ammonia.
