Abstract
Singapore
grouper
iridovirus
(SGIV)
was
first
isolated
from
diseased
cultured
groupers
in
and
has
been
identified
as
a
novel
species
within
the
genus
Ranavirus
(family
Iridoviridae
;
subfamily
Alphairidovirinae
).
SGIV
infection
causes
considerable
morbidity
mortality
many
economically
important
fish
species,
such
seabass.
In
this
chapter,
we
describe
virus
isolation
cell
culture,
virion
purification,
ultrastructural
analysis,
morphogenesis,
molecular
identification
of
SGIV.
molecularly
characterized
based
on
genome,
transcriptome,
proteome,
viral
miRNAs.
Various
aspects
pathogenesis
resulting
were
investigated,
including
cytopathology,
entry
transport,
paraptosis,
autophagy,
signaling
pathways.
Functions
host
immune
metabolism-related
genes
during
are
evaluated
discussed.
Immuno-biological
control
strategies,
antibody-based
flow
cytometry
microfluidic
chip
detection
technology,
loop-mediated
isothermal
amplification
(LAMP),
nucleic
acid
aptamer
methods,
developed.
Efficient
vaccines
have
also
These
research
approaches
provide
basis
for
better
understanding
other
ranaviruses
offer
technical
support
to
ranaviruses.
Journal of Fish Diseases,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 4, 2025
ABSTRACT
The
largemouth
bass
virus
(LMBV)
significantly
impacts
Chinese
aquaculture.
molecular
mechanisms
regulating
LMBV
virulence
and
the
gene
responses
stimulated
in
host
during
infection
remain
unclear.
This
study
investigates
transcriptional
dynamics
signalling
pathways
activated
immune
response
to
by
analysing
transcriptome
of
head
kidney
tissues
at
1,
4,
7,
28
days
post‐infection
(dpi)
using
RNA
sequencing.
Histopathological
viral
load
analyses
indicated
early
tissue
disruption,
followed
extensive
recovery
dpi.
Analysis
differentially
expressed
genes
(DEGs)
weighted
co‐expression
network
analysis
(WGCNA),
combined
with
Venn
samples
7
dpi,
identified
significant
common
genes.
Early
triggered
robust
innate
immunity
through
activation
RIG‐I
like
receptor
cGAS‐STING
pathways,
which
type
I
interferons
(IFNs)
interferon‐stimulated
(ISGs).
Later
stages
adaptive
responses.
Validation
randomly
selected
via
RT‐qPCR
confirmed
RNA‐seq
results,
showing
consistent
expression
patterns.
comprehensive
offers
new
insights
into
sustained
LMBV.
