Abstract
Singapore
grouper
iridovirus
(SGIV)
was
first
isolated
from
diseased
cultured
groupers
in
and
has
been
identified
as
a
novel
species
within
the
genus
Ranavirus
(family
Iridoviridae
;
subfamily
Alphairidovirinae
).
SGIV
infection
causes
considerable
morbidity
mortality
many
economically
important
fish
species,
such
seabass.
In
this
chapter,
we
describe
virus
isolation
cell
culture,
virion
purification,
ultrastructural
analysis,
morphogenesis,
molecular
identification
of
SGIV.
molecularly
characterized
based
on
genome,
transcriptome,
proteome,
viral
miRNAs.
Various
aspects
pathogenesis
resulting
were
investigated,
including
cytopathology,
entry
transport,
paraptosis,
autophagy,
signaling
pathways.
Functions
host
immune
metabolism-related
genes
during
are
evaluated
discussed.
Immuno-biological
control
strategies,
antibody-based
flow
cytometry
microfluidic
chip
detection
technology,
loop-mediated
isothermal
amplification
(LAMP),
nucleic
acid
aptamer
methods,
developed.
Efficient
vaccines
have
also
These
research
approaches
provide
basis
for
better
understanding
other
ranaviruses
offer
technical
support
to
ranaviruses.
Abstract
This
updated
review
is
presented
in
two
parts.
The
first,
based
primarily
on
work
conducted
since
the
discovery
of
first
ranavirus
mid-1960s
and
extending
through
2014,
summarizes
taxonomy,
viral
life
cycle,
impact
infection
host
cell,
immune
response
to
infection.
second
part,
drawing
research
mainly
edition
this
monograph
2015,
describes
genetic
molecular
approaches
for
determining
gene
function
outlines
role
products
orchestrating
events
leading
production
infectious
virions,
cytopathology,
inhibition
anti-viral
immunity.
Furthermore,
because
our
understanding
certain
replication
remains
be
elucidated,
areas
requiring
further
are
highlighted.
Abstract
Singapore
grouper
iridovirus
(SGIV)
was
first
isolated
from
diseased
cultured
groupers
in
and
has
been
identified
as
a
novel
species
within
the
genus
Ranavirus
(family
Iridoviridae
;
subfamily
Alphairidovirinae
).
SGIV
infection
causes
considerable
morbidity
mortality
many
economically
important
fish
species,
such
seabass.
In
this
chapter,
we
describe
virus
isolation
cell
culture,
virion
purification,
ultrastructural
analysis,
morphogenesis,
molecular
identification
of
SGIV.
molecularly
characterized
based
on
genome,
transcriptome,
proteome,
viral
miRNAs.
Various
aspects
pathogenesis
resulting
were
investigated,
including
cytopathology,
entry
transport,
paraptosis,
autophagy,
signaling
pathways.
Functions
host
immune
metabolism-related
genes
during
are
evaluated
discussed.
Immuno-biological
control
strategies,
antibody-based
flow
cytometry
microfluidic
chip
detection
technology,
loop-mediated
isothermal
amplification
(LAMP),
nucleic
acid
aptamer
methods,
developed.
Efficient
vaccines
have
also
These
research
approaches
provide
basis
for
better
understanding
other
ranaviruses
offer
technical
support
to
ranaviruses.
