Animals,
Journal Year:
2024,
Volume and Issue:
14(9), P. 1298 - 1298
Published: April 25, 2024
AOS
alleviates
inflammatory
responses;
however,
whether
it
exerts
an
effect
on
the
rumen
or
regulates
reaction
remains
unknown.
In
this
study,
firstly,
ovine
ruminal
epithelial
cells
(ORECs)
were
treated
with
0,
200,
400,
600,
and
800
µg/mL
AOS,
hoping
to
explore
hurt
cell
health.
The
results
showed
that
compared
AOS-0
group,
AOS-400
group
could
significantly
increase
(p
<
0.05)
viability,
reduce
reactive
oxygen
species
(ROS)
interleukin
(IL)-6
content,
have
no
adverse
cells.
Secondly,
we
used
LPS
construct
in
vitro
model
of
then
explored
protective
role
study
was
divided
into
three
groups:
control
(CON),
LPS,
+
AOS.
demonstrated
increased
viability
reduced
ROS
level
comparison
0.05).
Pretreatment
also
repressed
secretion
IL-1β,
IL-6,
IL-8,
immunoglobulin
(Ig)A
from
ORECs
culture
medium
following
LPS.
terms
tight
junction
(TJ)
proteins,
treatment
zonula
occludens
1
(ZO-1)
Occludin
expression.
apoptosis
rate,
Caspase3,
Caspase9,
BAD,
BCL-2/BAX
decreased
after
treatment,
expression
BCL-2
addition,
expressions
Toll-like
receptor
4
(TLR4),
myeloid
differentiation
factor
88
(MyD88),
nuclear
factor-κB
(NF-κB)
inhibited
addition
At
protein
level,
pretreatment
MyD88
phosphorylation
inhibitor
κB
α
(IκBα)
challenge.
Taken
together,
our
indicated
alleviate
LPS-induced
response
through
NF-κB
signaling
pathway,
which
may
be
a
promising
strategy
for
treating
inflammation
sheep
breeding.
Journal of Agricultural and Food Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 26, 2025
Alginate
lyases,
enzymes
that
degrade
alginate
into
unsaturated
oligosaccharides,
have
attracted
significant
attention
for
their
potential
applications
across
various
fields,
particularly
in
agriculture.
This
review
focuses
on
the
molecular
engineering
of
lyases
to
enhance
activity,
stability,
and
specificity
as
well
agricultural
resulting
enzymatic
products,
known
oligosaccharides
(AOS).
We
start
by
summarizing
sources
classification
followed
recent
advances
through
directed
evolution,
rational
design,
truncation
noncatalytic
domains,
conserved
domain
reconstruction.
then
explore
diverse
AOS,
including
ability
promote
plant
growth,
increase
content
active
components,
extend
fruit
shelf
life,
resistance
abiotic
stresses.
Furthermore,
value
AOS
feed
additives
preservatives
shrimp-based
products
is
also
assessed.
will
not
only
lay
a
solid
theoretical
foundation
but
serve
catalyst
innovative
development
practical
application
high-value
preparations
utilization
AOS-related
providing
new
solutions
sustainable
agriculture
food
industry.
Animals,
Journal Year:
2025,
Volume and Issue:
15(5), P. 622 - 622
Published: Feb. 20, 2025
This
study
used
largemouth
bass
(initial
average
weight:
33.33
±
1.8
g)
to
explore
the
effects
of
adding
different
brown
algae
extracts
feed
on
fish's
growth,
immunity
and
intestinal
health.
Six
groups
were
set
up:
a
control
(Group
A),
0.1%
sodium
alginate
B),
oligotriosaccharide
I
C),
II
D),
0.2%
powder
E)
enzymatic
product
F),
with
three
replicates
35
fish
each,
56-day
feeding
experiment.
Results:
Compared
Group
A,
Groups
C,
D
F
had
higher
specific
growth
rate
lower
coefficient
(p
<
0.05).
enhanced
serum
SOD
activity;
increased
antioxidant
enzyme
activity
decreased
MDA
content
All
experimental
LZM
levels
0.05),
no
IgM
difference
>
In
intestine,
treatment
α-amylase
0.05)
lipase
trypsin
tallest
villi,
B
thickest
muscular
layer
villus
width
was
similar
among
The
fewer
pathogenic
bacteria,
improved
microorganism
diversity
richness
conclusion,
can
promote
capacity
immunity.
is
better
for
development
flora
improvement.
Journal of Applied Phycology,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 23, 2025
Abstract
The
aquaculture
industry
is
a
rapidly
growing
sector
and
vital
source
of
nutrients,
with
projected
10%
increase
in
production
by
2032.
This
rapid
growth
has
prompted
the
need
to
identify
sustainable
feed
supplements,
as
many
aquatic
species
require
nutritionally
balanced
diets.
To
address
this,
brown
algae
have
emerged
promising
supplement
due
their
rich
content
carbohydrates,
fatty
acids,
minerals,
antioxidants,
other
bioactive
compounds,
which
can
improve
health
performance
farmed
species.
review
describes
algae,
main
well
use
feeds
using
different
inclusion
percentages
impact
on
zootechnical
(growth
rate,
weight,
mortality)
physiological
(digestive
activity,
immune
response,
among
others)
parameters
organisms.
In
addition,
various
processing
techniques
are
discussed,
including
hydrothermal,
enzymatic,
supercritical
fluid,
ultrasonic,
fermentation
methods,
bioavailability
reduce
antinutritional
factors.
challenges,
future
prospects,
economic
implications
integrating
into
also
highlighted.
Graphical
Food Quality and Safety,
Journal Year:
2024,
Volume and Issue:
8
Published: Jan. 1, 2024
Abstract
Objectives
Vibrio
parahaemolyticus
is
the
primary
species
that
causes
vibriosis.
In
this
study,
a
point-of-care
detection
method
was
developed
for
V.
parahaemolyticus.
Materials
and
Methods
The
platform
targeted
thermolabile
haemolysin
(tlh)
gene
of
based
on
recombinant
polymerase
amplification
(RPA)
clustered
regularly
spaced
short
palindromic
repeat
(CRISPR/Cas)
systems.
combined
with
an
immunochromatographic
test
strip
(ICS)
enables
low-cost,
simple,
visual
Results
limit
2.5×102
fg/µL
plasmids
1.4×102
CFU/mL
addition,
in
salmon
sashimi
could
be
detected
at
concentration
154
CFU/g
without
enrichment,
entire
time
around
30
min.
After
enrichment
6
h,
2
detected.
Conclusions
Consequently,
proposed
RPA-CRISPR/Cas12a-ICS
detect
seafood
intuitively,
quickly,
sensitively,
leading
to
high
practical
application
value.
