The Identification of Proteomic Signatures Associated with Alkaline Tolerance in the Skin Mucus of Crucian Carp (Carassius auratus) DOI Open Access
Zhipeng Sun, Jing Huang, Xiao Feng Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11618 - 11618

Published: Oct. 29, 2024

The skin is covered by a protective mucus layer, which essential to the innate defense mechanism of fish. Investigating response various toxic stresses crucial for enhancing its ability tackle environmental challenges and developing strategies mitigate effects. Alkalinity stress assays (50 mmol/L NaHCO3) were conducted on crucian carp (Carassius auratus) from Lake Dali Nur (pH = 9.6) Ping Xiang red freshwater 7) over 7 days. expression mucous proteins was analyzed using liquid chromatography (LC)-spectrometry (MS)/MS Analysis-Data-independent acquisition (DIA) mode. A total 12,537 identified across 20 samples four groups, with 12,025 quantified. In alkaline water population, high alkali resulted in up-regulation 139 down-regulation 500 proteins. contrast, population showed an increase 112 decrease 120; both populations had 23 genes up-regulated 21 down-regulated. protein regulatory network group included 3146 pairwise interactions among 464 nodes, only being differentially expressed Conversely, group’s comprised just 1027 specific 337 6 corresponding common responding extracted visualized populations. Based their relationships levels, these are hypothesized play similar roles under stress. Notably, alpha-globin fragment keratin type I cytoskeletal 13-like markedly expression, increasing nearly thousandfold extremely low level. This suggests it could serve as potential biomarker tolerance, warranting further investigation.

Language: Английский

Modulation of growth, immunity, and immune‐antioxidant gene expression in Nile tilapia, Oreochromis niloticus, culture under biofloc system by dragon fruit, Hylocereus undatus, peel powder DOI Creative Commons
Chinh Le Xuan,

Vu Linh Nguyen,

Supreya Wannavijit

et al.

Journal of the World Aquaculture Society, Journal Year: 2024, Volume and Issue: 55(5)

Published: June 27, 2024

Abstract This study examines the use of dragon fruit peel (DFP) powder as a dietary supplement on growth performance, immune responses, and gene expression Nile tilapia, Oreochromis niloticus , cultured within biofloc systems. A total 300 tilapia fingerlings (14.64 ± 0.09 g) were subjected to five treatments, with DFP added basal diet at doses 0, 20, 40, 80, 160 g kg −1 DFP. Growth immunological responses assessed after 4 8 weeks feeding, transcriptional level antioxidant‐related genes was measured weeks. Fish fed diets containing exhibited significantly greater weight gain, faster growth, enhanced levels key indicators immunity than control fish ( p < 0.05). 40 produced best result in terms response skin mucus blood serum, upregulation IL‐1 IL‐8 LBP GPx GST‐α GSR Based quadratic regression analysis, optimum concentration 80 . These findings collectively suggest that powdered may serve promising feed for raised

Language: Английский

Citations

3
Effects of artichoke (Cynara scolymus) polyphenolic extract on growth, antioxidants and some immune parameters in common carp (Cyprinus carpio) DOI Creative Commons
Eshrat Beigom Kia, Seyed Hossein Hoseinifar, Mohammad Mazandarani

et al.

BMC Veterinary Research, Journal Year: 2025, Volume and Issue: 21(1)

Published: March 17, 2025

In this research, Cyprinus carpio juveniles were fed with experimental diets containing different levels of artichoke polyphenolic leaf extract (ALE) 0 (Control), 0.5, 1, and 2 g/kg over eight weeks. After weeks, haemato-immunological parameters, growth performance, immune parameters antioxidant defense measured. The administration varying ALE had no significant effects on compared to the control group. However, haematological significantly elevated Immune such as serum lysozyme, mucus total immunoglobulin, well alternative complement activity bactericidal ALE-treated carps, showed a notable increase in comparison CTRL Also, agglutination titer carps higher than Feeding (0.5, g/kg) enhanced activities enzymes, including superoxide dismutase all groups, catalase 0.5 glutathione peroxidase 1 results study demonstrate that dietary supplementation at 0.5–2 positively influences factors carpio, most pronounced observed g/kg. These findings suggest extract, particularly dosages, has potential an effective feed additive enhance immunity capacity carp farming.

Language: Английский

Citations

0
Seventy years of coexistence: Parasites and Mediterranean fish aquaculture DOI
Ivona Mladineo, Jerko Hrabar

Fish & Shellfish Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 110355 - 110355

Published: April 1, 2025

Language: Английский

Citations

0
LncRNA transcriptome analysis of rainbow trout ( Oncorhynchus mykiss ) skin infected with IHNV reveals that lncRNA SARL/miR-205-z/ SOCS3 axis negatively regulates antiviral immunity mechanisms DOI Creative Commons
Lu Zhao, Jinqiang Huang, Yongjuan Li

et al.

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 27, 2025

Long non-coding RNAs (lncRNAs) are new gene regulators involved in various biological processes. However, the regulatory effect of lncRNA on rainbow trout (Oncorhynchus mykiss) antiviral immune response has not been reported. Here, we measured profiles at 48 hpi compared to control group, expression levels lncRNA, miRNA, and gene, SARL/miR-205-z/SOCS3 functions after skin infected with infectious haematopoietic necrosis virus (IHNV) by RNA-seq, qRT-PCR, overexpression inhibition assays. Transcriptome analysis identified twelve upregulated four downregulated DElncRNAs. Twelve key immune-related competing endogenous RNA (ceRNA) networks were identified, target genes enriched TLR, RLR, NLR, p53 signalling pathways. Expression patterns suggested that changes SARL, miR-205-z, SOCS3 presented a ceRNA relationship. Further studies demonstrated SARL was sponging miR-205-z vitro, thereby playing negative role trout. We also found positive regulator liver cell proliferation, this could be reversed SOCS3. In vivo, significantly increased antagomiR-205-z injection. Furthermore, promoted replication IHNV. This study provides fundamental data for disease resistance breeding targeted drug therapy

Language: Английский

Citations

0
The Identification of Proteomic Signatures Associated with Alkaline Tolerance in the Skin Mucus of Crucian Carp (Carassius auratus) DOI Open Access
Zhipeng Sun, Jing Huang, Xiao Feng Zhang

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11618 - 11618

Published: Oct. 29, 2024

The skin is covered by a protective mucus layer, which essential to the innate defense mechanism of fish. Investigating response various toxic stresses crucial for enhancing its ability tackle environmental challenges and developing strategies mitigate effects. Alkalinity stress assays (50 mmol/L NaHCO3) were conducted on crucian carp (Carassius auratus) from Lake Dali Nur (pH = 9.6) Ping Xiang red freshwater 7) over 7 days. expression mucous proteins was analyzed using liquid chromatography (LC)-spectrometry (MS)/MS Analysis-Data-independent acquisition (DIA) mode. A total 12,537 identified across 20 samples four groups, with 12,025 quantified. In alkaline water population, high alkali resulted in up-regulation 139 down-regulation 500 proteins. contrast, population showed an increase 112 decrease 120; both populations had 23 genes up-regulated 21 down-regulated. protein regulatory network group included 3146 pairwise interactions among 464 nodes, only being differentially expressed Conversely, group’s comprised just 1027 specific 337 6 corresponding common responding extracted visualized populations. Based their relationships levels, these are hypothesized play similar roles under stress. Notably, alpha-globin fragment keratin type I cytoskeletal 13-like markedly expression, increasing nearly thousandfold extremely low level. This suggests it could serve as potential biomarker tolerance, warranting further investigation.

Language: Английский

Citations

1