Effects of hypoxia and reoxygenation on energy metabolism, immune response, and apoptosis in orange-spotted grouper (Epinephelus coioides) DOI Creative Commons

Yuxin Wu,

Yiran Lin,

Lin Bing

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 13, 2024

Hypoxia is an unfavorable environmental condition that produces diverse negative effects in fish. High-density cultures of Epinephelus coioides are more likely to experience hypoxic conditions than those natural environments. To assess the hypoxia on E. , we examined related enzyme activities and gene expression after 48 h 24 dissolved oxygen (DO) recovery. Under stress (DO: 1.2 ± 0.1 mg/L), energy supply mode fish changed from aerobic metabolism anaerobic metabolism, serum glucose content lactate dehydrogenase activity were significantly upregulated. Total protein, hepatic glycogen, two key regulatory enzymes (i.e., hexokinase pyruvate kinase) differentially expressed liver, mRNA three genes LDHA GLUT1 MCT2 ) also showed a high trend. In serum, immune-related alanine aminotransferase, aspartate alkaline phosphatase) found be involved regulation by different levels changing patterns. Expression inflammatory IL-8 IFNγ MyD88 NF-kB regulated liver. With prolongation stress, apoptotic p53 Bax Bcl-2 Caspase-9 was closely degree apoptosis Our investigation changes immune response, under reoxygenation (DO, 6.0 mg/L) provides theoretical bases for healthy aquaculture selection varieties with tolerance hypoxia.

Language: Английский

Supplementation of Bacillus velezensis S141 in feed as a probiotic enhances growth performance, pathogenic tolerances, and immune system in shrimp DOI

Tuangrak Seabkongseng,

Sirawich Limkul, Cheeranan Sriphuttha

et al.

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742448 - 742448

Published: March 1, 2025

Language: Английский

Citations

0
Commensal microorganisms ameliorate the adverse effects of high wheat starch diet on the growth performance, glucose and lipid metabolisms in juvenile largemouth bass, Micropterus salmoides DOI Creative Commons
Biyun Zhang, Hong‐Ling Yang,

Ze-Hui Yuan

et al.

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 39, P. 102452 - 102452

Published: Oct. 28, 2024

Language: Английский

Citations

0
Functional potential of compound probiotics fermented soybean meal partially replacing fish meal in spotted seabass (Lateolabrax maculatus): Insights through growth, immunity, antioxidant capacity and intestinal health DOI

B Zhang,

Ze-Hui Yuan,

Haixia Yu

et al.

Aquaculture, Journal Year: 2024, Volume and Issue: 598, P. 742021 - 742021

Published: Dec. 10, 2024

Language: Английский

Citations

0
Effects of hypoxia and reoxygenation on energy metabolism, immune response, and apoptosis in orange-spotted grouper (Epinephelus coioides) DOI Creative Commons

Yuxin Wu,

Yiran Lin,

Lin Bing

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 13, 2024

Hypoxia is an unfavorable environmental condition that produces diverse negative effects in fish. High-density cultures of Epinephelus coioides are more likely to experience hypoxic conditions than those natural environments. To assess the hypoxia on E. , we examined related enzyme activities and gene expression after 48 h 24 dissolved oxygen (DO) recovery. Under stress (DO: 1.2 ± 0.1 mg/L), energy supply mode fish changed from aerobic metabolism anaerobic metabolism, serum glucose content lactate dehydrogenase activity were significantly upregulated. Total protein, hepatic glycogen, two key regulatory enzymes (i.e., hexokinase pyruvate kinase) differentially expressed liver, mRNA three genes LDHA GLUT1 MCT2 ) also showed a high trend. In serum, immune-related alanine aminotransferase, aspartate alkaline phosphatase) found be involved regulation by different levels changing patterns. Expression inflammatory IL-8 IFNγ MyD88 NF-kB regulated liver. With prolongation stress, apoptotic p53 Bax Bcl-2 Caspase-9 was closely degree apoptosis Our investigation changes immune response, under reoxygenation (DO, 6.0 mg/L) provides theoretical bases for healthy aquaculture selection varieties with tolerance hypoxia.

Language: Английский

Citations

0