Synbiotic-driven modulation of the gut microbiota and metabolic functions related to obesity: insights from a human gastrointestinal model DOI Creative Commons
Kevin Mok,

Putsawee Tomtong,

Takuma Ogawa

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 27, 2025

Synbiotic interventions have gained increasing attention for modulating gut microbiota and metabolic functions in obesity-related disorders. This study evaluated the effects of Limosilactobacillus reuteri KUB-AC5 (10⁸ CFU) Wolffia globosa powder (6 g/day) using an vitro continuous human gastrointestinal model. Fecal samples from obese donors were used to simulate ascending descending colon, with microbial viability, diversity, metabolite production assessed over 14 days via culture-dependent culture-independent methods. supplementation increased anaerobic bacterial counts by 2.6 log CFU/mL colon 2.2 notable increases lactic acid bacteria reductions Enterobacteriaceae. Metagenomic analysis revealed trend diversity evenness after 7 treatment, though changes not statistically significant. PERMANOVA confirmed significant shift community composition between stabilization, washout periods (p < 0.05). Additionally, butyrate levels significantly 0.05), while p-cresol, a deleterious metabolite, decreased Bile was modulated, tertiary bile 3-oxo-LCA enhanced deconjugation, suggesting improved lipid metabolism potential weight management benefits. These findings highlight synbiotic enhance beneficial populations, improve support health obesity management.

Language: Английский

Synbiotic-driven modulation of the gut microbiota and metabolic functions related to obesity: insights from a human gastrointestinal model DOI Creative Commons
Kevin Mok,

Putsawee Tomtong,

Takuma Ogawa

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 27, 2025

Synbiotic interventions have gained increasing attention for modulating gut microbiota and metabolic functions in obesity-related disorders. This study evaluated the effects of Limosilactobacillus reuteri KUB-AC5 (10⁸ CFU) Wolffia globosa powder (6 g/day) using an vitro continuous human gastrointestinal model. Fecal samples from obese donors were used to simulate ascending descending colon, with microbial viability, diversity, metabolite production assessed over 14 days via culture-dependent culture-independent methods. supplementation increased anaerobic bacterial counts by 2.6 log CFU/mL colon 2.2 notable increases lactic acid bacteria reductions Enterobacteriaceae. Metagenomic analysis revealed trend diversity evenness after 7 treatment, though changes not statistically significant. PERMANOVA confirmed significant shift community composition between stabilization, washout periods (p < 0.05). Additionally, butyrate levels significantly 0.05), while p-cresol, a deleterious metabolite, decreased Bile was modulated, tertiary bile 3-oxo-LCA enhanced deconjugation, suggesting improved lipid metabolism potential weight management benefits. These findings highlight synbiotic enhance beneficial populations, improve support health obesity management.

Language: Английский

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