Assessing the In Vitro Individual and Combined Effect of Arthrobotrys Oligospora and <em>A. musiformis</em> (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode <em>Haemonchus contortus</em> (Trichostrongylidae)
Published: April 17, 2024
The
individual
and
combined
effects
of
extracellular
products
(ECP)
from
Arthrobotrys
oligospora
(Ao)
A.
musiformis
(Am)
growth
in
liquid
media
against
Haemonchus
contortus
L3
(HcL3)
were
assessed.
following
methodological
steps
established:
isolation,
morphological
(MI)
molecular
identification
(Mol-I),
assessment
nematocidal
activity
(NA)
fungal
liq-uid
culture
filtrates
(LCF)
produced
two
alone
combination
finally,
the
myco-compound
profile
(MCP).
MI
both
isolates
suggested
that
fungi
corresponded
to
species
Ao
Am.
This
result
was
confirmed
by
PCR
analysis
followed
sequencing,
alignment
obtaining
coverage
similarity
with
respect
reported
NCBI
database.
Likewise,
highest
Hc
mortalities
91.4%
LCF
Am
86.2%
those
at
concentration
(100
mg/mL)
Czapek-Dox
Broth.
resulted
a
similarly
high
larval
mortality
no
statistical
differ-ences
relation
(P>0.05).
MCP
showed
presence
alkaloids
fungi.
Coumarins,
triterpenes
saponins
found
only
Ao.
Both
ECP
NA
could
be
identified
assessed
future
studies
as
potential
natural
an-thelmintic
compounds.
Language: Английский
In vitro ovicidal and larvicidal activity of a hydroalcoholic extract and its fractions from Cyrtocarpa procera fruits on Haemonchus contortus
Experimental Parasitology,
Journal Year:
2024,
Volume and Issue:
262, P. 108777 - 108777
Published: May 11, 2024
Language: Английский
Arthrobotrys oligospora (Fungi: Orbiliales) and its liquid culture filtrate myco-constituents kill Haemonchus contortus infective larvae (Nematoda: trichostrongylidae)
Biocontrol Science and Technology,
Journal Year:
2024,
Volume and Issue:
34(8), P. 754 - 775
Published: July 15, 2024
Arthrobotrys
oligospora
is
a
nematode-trapping
fungus
belonging
to
the
family
Orbiliaceae
and
one
of
main
antagonists
nematodes
in
soil,
including
plant
animal
parasitic
free-living
nematodes.
This
species
develops
three-dimensional
adhesive
nets
which
are
trapped,
destroyed
consumed
by
fungus.
It
also
possesses
other
strategies
immobilise,
kill
degrade
nematodes,
i.e.
nematode-attractant
substances,
cuticle
adhesion
polymers
number
nematocidal
metabolites.
The
objective
this
study
was
assess
vitro
activity
strain
A.
through
its
predatory
liquid
culture
filtrates
(LCF)
against
ruminant
parasite
Haemonchus
contortus
(HcL3)
investigate
presence
myco-compounds
with
potential
activity.
Predatory
(PA)
assessed
agar
plates;
200
HcL3
were
placed
on
plates
control
group
incubated
for
10
days.
LCF
confrontation
performed
microtiter
plates.
Two
media,
Czapek-Dox
Broth
(Cz-DB)
potato
dextrose
broth,
assessed.
Three
concentrations
(25,
50
100
mg/mL)
at
48
72
h
(n
=
4,
per
concentration).
experiment
triplicate.
results
showed
73.6
±
2.7%
PA
highest
mortality
(83%)
Cz-DB
after
h.
Gas
chromatography–mass
spectrometry
(GC–MS)
analysis
revealed
that
ethyl
acetate
phase
composed
1,2-benzenedicarboxylic
acid,
bis
(2-methylpropyl)
ester,
(M)
1,4-benzenedicarboxylic
ester
decanedioic
acid
(2-ethylexyl)
ester.
These
compounds
could
be
responsible
Language: Английский
Assessing the In Vitro Individual and Combined Effect of Arthrobotrys oligospora and A. musiformis (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode Haemonchus contortus (Trichostrongylidae)
Pathogens,
Journal Year:
2024,
Volume and Issue:
13(6), P. 498 - 498
Published: June 11, 2024
Background:
Arthrobotrys
species
are
nematophagous
fungi
that
secrete
extracellular
nematocidal
products
(ECP).
The
individual
and
combined
effects
of
ECP
from
oligospora
(Ao)
A.
musiformis
(Am)
growth
in
liquid
media
against
Haemonchus
contortus
L3
(HcL3)
were
assessed.
Methods:
isolation,
morphological
(MI)
molecular
identification
(Mol-I),
assessment
activity
(NA)
fungal
culture
filtrates
(LCF)
two
alone
combination
the
myco-compound
profile
(MCP)
performed.
Results:
MI
suggested
corresponded
to
Ao
Am.
This
result
was
confirmed
by
PCR
analysis
followed
sequencing,
alignment
a
phylogenetic
analysis.
Likewise,
highest
Hc
mortalities
91.4%
with
LCF
Am
86.2%
those
at
concentration
(100
mg/mL)
Czapek-Dox
Broth.
both
resulted
similarly
high
larval
mortality
no
statistical
differences
relation
(p
>
0.05).
MCP
showed
presence
alkaloids
fungi.
Coumarins,
sterols
saponins
found
only
Ao.
Main
conclusions:
Both
produced
NA
could
be
identified
assessed
future
studies
as
potential
natural
anthelmintic
compounds.
Language: Английский
Arthrobotrys mendozadegivensis sp. nov. (Fungi: Orbiliales) from Mexico: Predatory Activity and Nematocidal Activity of Its Liquid Culture Filtrates Against Haemonchus contortus (Nematoda: Trichostrongylidae)
Journal of Fungi,
Journal Year:
2024,
Volume and Issue:
10(12), P. 888 - 888
Published: Dec. 22, 2024
During
the
isolation,
identification,
and
assessment
of
nematode-trapping
fungi
(NTF)
against
nematodes,
we
discovered
an
unusual
fungus
in
decaying
wood
from
Morelos
State,
Mexico.
This
isolate
exhibited
some
characteristics
similar
to
those
Arthrobotrys
genus;
however,
found
that
it
did
not
match
any
previously
reported
species
within
this
genus
after
conducting
morphological
phylogenetic
analyses
using
ITS,
TEF,
RPB2
regions.
new
displays
conidiophores
with
two
or
three
stems
emerging
same
initial
site
only
a
single
stem
aerial
thickened
hyphae
which
emerge,
forming
3D
adhesive
nets.
The
conidia,
have
one
septa,
range
obovoid
ellipsoidal,
crowned
by
four
six
conidia.
report
provides
evidence
has
been
described
before,
hereby
introduce
as
species,
naming
mendozadegivensis.
displayed
predatory
activity
76.92%,
its
liquid
culture
filtrates
Sweet
Potato
Dextrose
Broth
Czapek–Dox
were
effective
killing
40.90%
34.91%
Haemonchus
contortus
larvae,
respectively.
study
information
about
unreported
nematophagous
fungus,
is
important
for
systematics
potential
biotechnological
applications
nematodes
affect
livestock
industry.
Language: Английский